Water-insensitive NIR-I-to-NIR-I down-shifting nanoparticles enable stable biomarker detection at low power thresholds in opaque aqueous environments
Abstract Luminescence quenching in aqueous environments poses a challenge for practical applications. Lanthanide-doped up-conversion nanoparticles (UCNPs), representative of near-infrared (NIR)-emitting phosphors, typically utilize Yb3+ ions as sensitizers, requiring 980 nm light. This wavelength co...
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| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Publishing Group
2025-07-01
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| Series: | Light: Science & Applications |
| Online Access: | https://doi.org/10.1038/s41377-025-01882-2 |
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| Summary: | Abstract Luminescence quenching in aqueous environments poses a challenge for practical applications. Lanthanide-doped up-conversion nanoparticles (UCNPs), representative of near-infrared (NIR)-emitting phosphors, typically utilize Yb3+ ions as sensitizers, requiring 980 nm light. This wavelength coincides with the transitions of water molecules, interfering with population dynamics, and continuous irradiation causes unintended heating. Although Nd3+ ions, which absorb at 800 nm, serve as alternative sensitizers, their practical use is limited by low quantum yield (Q.Y.). In this study, we developed water-insensitive down-shifting nanoparticles (WINPs) functioning within the NIR-I range (700–900 nm) to avoid water interference. Characterization through single-particle-level spectroscopy demonstrated water-insensitive properties, with identical powers density and lifetime profiles under both dry and water conditions. The WINPs achieved a high Q.Y. of 22.1 ± 0.9%, allowing operation at a detection limit power 15-fold lower than UCNPs, effectively eliminating background noise and enhancing overall performance. To assess diagnostic potential, we validated WINP-based lateral flow immunoassay (LFA) for detecting avian influenza viruses (AIVs) in 65 opaque clinical samples, achieving 100% sensitivity and an area under the curve (AUC) of 1.000 at only 100 mW cm−2. These findings highlight the potential of WINPs as water-insensitive NIR phosphors that can operate at low power, even in water-rich environments. |
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| ISSN: | 2047-7538 |