Improved Stem Cell MR Detectability in Animal Models by Modification of the Inhalation Gas
In vivo monitoring of cells labeled with paramagnetic iron oxide particles by magnetic resonance imaging (MRI) is complicated by intrinsic contrast of blood vessels. Distinction between T 2 * effects caused by blood vessels from those caused by labeled cells was so far only possible after carefully...
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| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2005-04-01
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| Series: | Molecular Imaging |
| Online Access: | https://doi.org/10.1162/15353500200504196 |
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| _version_ | 1841564605818601472 |
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| author | Uwe Himmelreich Ralph Weber Pedro Ramos-Cabrer Susanne Wegener Korinna Kandal Eric M. Shapiro Alan P. Koretsky Mathias Hoehn |
| author_facet | Uwe Himmelreich Ralph Weber Pedro Ramos-Cabrer Susanne Wegener Korinna Kandal Eric M. Shapiro Alan P. Koretsky Mathias Hoehn |
| author_sort | Uwe Himmelreich |
| collection | DOAJ |
| description | In vivo monitoring of cells labeled with paramagnetic iron oxide particles by magnetic resonance imaging (MRI) is complicated by intrinsic contrast of blood vessels. Distinction between T 2 * effects caused by blood vessels from those caused by labeled cells was so far only possible after carefully following the location of hypointense regions through subsequent slices of T 2 *-weighted 3-D MRI datasets, a procedure that is time consuming and not always reliable in the case of smaller blood vessels. Here, we demonstrate that the modification of the inhalation gas mixture from the routinely used composition 35% O 2 and 65% N 2 O to a mixture containing 95% O 2 and 5% CO 2 results in a contrast suppression of most small blood vessels and reduces the intrinsic T 2 * effect of large vessels dramatically in an animal model. This change in protocol of physiological conditions was well tolerated by all studied animals, even over prolonged experimental times. The changed inhalation gas mixture thus provides a more reliable identification method for small clusters of iron oxide labeled cells in vivo. |
| format | Article |
| id | doaj-art-edbcb94bc1cd435491cf455adbaea474 |
| institution | Kabale University |
| issn | 1536-0121 |
| language | English |
| publishDate | 2005-04-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Molecular Imaging |
| spelling | doaj-art-edbcb94bc1cd435491cf455adbaea4742025-01-02T22:37:56ZengSAGE PublishingMolecular Imaging1536-01212005-04-01410.1162/1535350020050419610.1162_15353500200504196Improved Stem Cell MR Detectability in Animal Models by Modification of the Inhalation GasUwe Himmelreich0Ralph Weber1Pedro Ramos-Cabrer2Susanne Wegener3Korinna Kandal4Eric M. Shapiro5Alan P. Koretsky6Mathias Hoehn7Max-Planck-Institute for Neurological Research, Cologne, GermanyMax-Planck-Institute for Neurological Research, Cologne, GermanyMax-Planck-Institute for Neurological Research, Cologne, GermanyMax-Planck-Institute for Neurological Research, Cologne, GermanyMax-Planck-Institute for Neurological Research, Cologne, GermanyNew York University School of MedicineNational Institutes of Health, Bethesda, MDMax-Planck-Institute for Neurological Research, Cologne, GermanyIn vivo monitoring of cells labeled with paramagnetic iron oxide particles by magnetic resonance imaging (MRI) is complicated by intrinsic contrast of blood vessels. Distinction between T 2 * effects caused by blood vessels from those caused by labeled cells was so far only possible after carefully following the location of hypointense regions through subsequent slices of T 2 *-weighted 3-D MRI datasets, a procedure that is time consuming and not always reliable in the case of smaller blood vessels. Here, we demonstrate that the modification of the inhalation gas mixture from the routinely used composition 35% O 2 and 65% N 2 O to a mixture containing 95% O 2 and 5% CO 2 results in a contrast suppression of most small blood vessels and reduces the intrinsic T 2 * effect of large vessels dramatically in an animal model. This change in protocol of physiological conditions was well tolerated by all studied animals, even over prolonged experimental times. The changed inhalation gas mixture thus provides a more reliable identification method for small clusters of iron oxide labeled cells in vivo.https://doi.org/10.1162/15353500200504196 |
| spellingShingle | Uwe Himmelreich Ralph Weber Pedro Ramos-Cabrer Susanne Wegener Korinna Kandal Eric M. Shapiro Alan P. Koretsky Mathias Hoehn Improved Stem Cell MR Detectability in Animal Models by Modification of the Inhalation Gas Molecular Imaging |
| title | Improved Stem Cell MR Detectability in Animal Models by Modification of the Inhalation Gas |
| title_full | Improved Stem Cell MR Detectability in Animal Models by Modification of the Inhalation Gas |
| title_fullStr | Improved Stem Cell MR Detectability in Animal Models by Modification of the Inhalation Gas |
| title_full_unstemmed | Improved Stem Cell MR Detectability in Animal Models by Modification of the Inhalation Gas |
| title_short | Improved Stem Cell MR Detectability in Animal Models by Modification of the Inhalation Gas |
| title_sort | improved stem cell mr detectability in animal models by modification of the inhalation gas |
| url | https://doi.org/10.1162/15353500200504196 |
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