Matrix vesicle-inspired delivery system based on nanofibrous chitosan microspheres for enhanced bone regeneration
Inspired by the initial mineralization process with bone matrix vesicles (MVs), this study innovatively developed a delivery system to mediate mineralization during bone regeneration. The system comprises nanofibrous chitosan microspheres (NCM) and poly (allylamine hydrochloride)-stabilized amorphou...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-02-01
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| Series: | Materials Today Bio |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590006425000067 |
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| author | Haolin Bian Fangfang Song Shilei Wang Wei Sun Bo Hu Xichao Liang Hongye Yang Cui Huang |
| author_facet | Haolin Bian Fangfang Song Shilei Wang Wei Sun Bo Hu Xichao Liang Hongye Yang Cui Huang |
| author_sort | Haolin Bian |
| collection | DOAJ |
| description | Inspired by the initial mineralization process with bone matrix vesicles (MVs), this study innovatively developed a delivery system to mediate mineralization during bone regeneration. The system comprises nanofibrous chitosan microspheres (NCM) and poly (allylamine hydrochloride)-stabilized amorphous calcium phosphate (PAH-ACP), which is thereafter referred to as NCMP. NCM is synthesized through the thermal induction of chitosan molecular chains, serving as the carrier, while PAH-ACP functions as the mineralization precursor. Additionally, the nanofibrous network of NCMP mimics the architecture of natural extracellular matrix (ECM), creating an optimal niche for the active adhesion of stem cells to its surface, exhibiting good biocompatibility, immunoregulation, and osteogenic performance. In vivo, NCMP effectively recruits cells and mineralizes collagen, modulates cell behavior and differentiation, and promotes in situ biomineralization in rat calvarial defects. These results underscore the dual efficacy of NCMP not only as an effective delivery system for mineralization precursors but also as ECM-mimicking bio-blocks, offering a promising avenue for enhancing the repair and regeneration of bone defects. |
| format | Article |
| id | doaj-art-edba6bc1b5e54d7d8d80a32bba3ffe0a |
| institution | DOAJ |
| issn | 2590-0064 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Materials Today Bio |
| spelling | doaj-art-edba6bc1b5e54d7d8d80a32bba3ffe0a2025-08-20T03:01:03ZengElsevierMaterials Today Bio2590-00642025-02-013010144810.1016/j.mtbio.2025.101448Matrix vesicle-inspired delivery system based on nanofibrous chitosan microspheres for enhanced bone regenerationHaolin Bian0Fangfang Song1Shilei Wang2Wei Sun3Bo Hu4Xichao Liang5Hongye Yang6Cui Huang7State Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology, School & Hospital of Stomatology, Wuhan University, Wuhan, 430079, China; Department of Stomatology, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, 310003, ChinaState Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology, School & Hospital of Stomatology, Wuhan University, Wuhan, 430079, ChinaKey Laboratory of Resources and Compound of Traditional Chinese Medicine, Ministry of Education, Hubei University of Traditional Chinese Medicine, Wuhan, 430065, ChinaState Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology, School & Hospital of Stomatology, Wuhan University, Wuhan, 430079, ChinaResearch and Application of Regenerative Cellulose Fiber Key Laboratory of Sichuan Province, YiBin Grace Group Co., LTD, Yibin, 644000, ChinaResearch and Application of Regenerative Cellulose Fiber Key Laboratory of Sichuan Province, YiBin Grace Group Co., LTD, Yibin, 644000, ChinaState Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology, School & Hospital of Stomatology, Wuhan University, Wuhan, 430079, China; Corresponding author.State Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology, School & Hospital of Stomatology, Wuhan University, Wuhan, 430079, China; Corresponding author.Inspired by the initial mineralization process with bone matrix vesicles (MVs), this study innovatively developed a delivery system to mediate mineralization during bone regeneration. The system comprises nanofibrous chitosan microspheres (NCM) and poly (allylamine hydrochloride)-stabilized amorphous calcium phosphate (PAH-ACP), which is thereafter referred to as NCMP. NCM is synthesized through the thermal induction of chitosan molecular chains, serving as the carrier, while PAH-ACP functions as the mineralization precursor. Additionally, the nanofibrous network of NCMP mimics the architecture of natural extracellular matrix (ECM), creating an optimal niche for the active adhesion of stem cells to its surface, exhibiting good biocompatibility, immunoregulation, and osteogenic performance. In vivo, NCMP effectively recruits cells and mineralizes collagen, modulates cell behavior and differentiation, and promotes in situ biomineralization in rat calvarial defects. These results underscore the dual efficacy of NCMP not only as an effective delivery system for mineralization precursors but also as ECM-mimicking bio-blocks, offering a promising avenue for enhancing the repair and regeneration of bone defects.http://www.sciencedirect.com/science/article/pii/S2590006425000067Nanofibrous chitosan microsphereMatrix vesiclesAmorphous calcium phosphateCollagen mineralizationOsteogenesisTissue engineering |
| spellingShingle | Haolin Bian Fangfang Song Shilei Wang Wei Sun Bo Hu Xichao Liang Hongye Yang Cui Huang Matrix vesicle-inspired delivery system based on nanofibrous chitosan microspheres for enhanced bone regeneration Materials Today Bio Nanofibrous chitosan microsphere Matrix vesicles Amorphous calcium phosphate Collagen mineralization Osteogenesis Tissue engineering |
| title | Matrix vesicle-inspired delivery system based on nanofibrous chitosan microspheres for enhanced bone regeneration |
| title_full | Matrix vesicle-inspired delivery system based on nanofibrous chitosan microspheres for enhanced bone regeneration |
| title_fullStr | Matrix vesicle-inspired delivery system based on nanofibrous chitosan microspheres for enhanced bone regeneration |
| title_full_unstemmed | Matrix vesicle-inspired delivery system based on nanofibrous chitosan microspheres for enhanced bone regeneration |
| title_short | Matrix vesicle-inspired delivery system based on nanofibrous chitosan microspheres for enhanced bone regeneration |
| title_sort | matrix vesicle inspired delivery system based on nanofibrous chitosan microspheres for enhanced bone regeneration |
| topic | Nanofibrous chitosan microsphere Matrix vesicles Amorphous calcium phosphate Collagen mineralization Osteogenesis Tissue engineering |
| url | http://www.sciencedirect.com/science/article/pii/S2590006425000067 |
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