Assessment of Genetic Variation in Apis nigrocincta (Hymenoptera: Apidae) in Sulawesi Revealed by Partial Mitochondrial Cytochrome Oxidase I Gene Sequences
Asian cavity-nesting honey bee Apis nigrocincta, a native bee species from Sulawesi and the Philippines, plays a vital role in pollinating flowering plants in local ecosystem and agriculture. In this study, we assessed the intraspecific genetic variation of A. nigrocincta using the sequence of cytoc...
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Wiley
2020-01-01
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| Series: | Scientifica |
| Online Access: | http://dx.doi.org/10.1155/2020/1609473 |
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| author | Christian A. Lombogia Jimmy Posangi Hard N. Pollo Max Tulung Trina E. Tallei |
| author_facet | Christian A. Lombogia Jimmy Posangi Hard N. Pollo Max Tulung Trina E. Tallei |
| author_sort | Christian A. Lombogia |
| collection | DOAJ |
| description | Asian cavity-nesting honey bee Apis nigrocincta, a native bee species from Sulawesi and the Philippines, plays a vital role in pollinating flowering plants in local ecosystem and agriculture. In this study, we assessed the intraspecific genetic variation of A. nigrocincta using the sequence of cytochrome c oxidase subunit I (COI). Molecular phylogenetic analysis showed that there were three main clades in A. nigrocincta specimens from Sulawesi based on their respective locations (North, Central, and South Sulawesi). Genetic distance analysis using the Kimura 2-parameter (K2P) model showed that the intraspecific genetic distance in Sulawesi specimens ranged from 0.000 to 0.055. There are 26 nucleotide polymorphic sites within Sulawesi A. nigrocincta. The variation was dominated by transition T ↔ C. The molecular identification result was supported by morphological identification. The results of the two methods agree that the specimen under study was A. nigrocincta. The result of genetic distance calculation indicated that although the tested specimens were derived from remote locations, the genetic variation was still within the range of intraspecific variation. |
| format | Article |
| id | doaj-art-edb9391baff2410a9014d61ea5e024d5 |
| institution | DOAJ |
| issn | 2090-908X |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Scientifica |
| spelling | doaj-art-edb9391baff2410a9014d61ea5e024d52025-08-20T03:20:43ZengWileyScientifica2090-908X2020-01-01202010.1155/2020/16094731609473Assessment of Genetic Variation in Apis nigrocincta (Hymenoptera: Apidae) in Sulawesi Revealed by Partial Mitochondrial Cytochrome Oxidase I Gene SequencesChristian A. Lombogia0Jimmy Posangi1Hard N. Pollo2Max Tulung3Trina E. Tallei4Entomology Study Program, Postgraduate Program, Universitas Sam Ratulangi, Manado, North Sulawesi, IndonesiaPublic Health Study Program, Faculty of Public Health, Universitas Sam Ratulangi, Manado, North Sulawesi, IndonesiaForestry Science Study Program, Faculty of Agriculture, Universitas Sam Ratulangi, Manado, North Sulawesi, IndonesiaEntomology Study Program, Postgraduate Program, Universitas Sam Ratulangi, Manado, North Sulawesi, IndonesiaDepartment of Biology, Faculty of Mathematics and Natural Sciences, Universitas Sam Ratulangi, Manado, North Sulawesi, IndonesiaAsian cavity-nesting honey bee Apis nigrocincta, a native bee species from Sulawesi and the Philippines, plays a vital role in pollinating flowering plants in local ecosystem and agriculture. In this study, we assessed the intraspecific genetic variation of A. nigrocincta using the sequence of cytochrome c oxidase subunit I (COI). Molecular phylogenetic analysis showed that there were three main clades in A. nigrocincta specimens from Sulawesi based on their respective locations (North, Central, and South Sulawesi). Genetic distance analysis using the Kimura 2-parameter (K2P) model showed that the intraspecific genetic distance in Sulawesi specimens ranged from 0.000 to 0.055. There are 26 nucleotide polymorphic sites within Sulawesi A. nigrocincta. The variation was dominated by transition T ↔ C. The molecular identification result was supported by morphological identification. The results of the two methods agree that the specimen under study was A. nigrocincta. The result of genetic distance calculation indicated that although the tested specimens were derived from remote locations, the genetic variation was still within the range of intraspecific variation.http://dx.doi.org/10.1155/2020/1609473 |
| spellingShingle | Christian A. Lombogia Jimmy Posangi Hard N. Pollo Max Tulung Trina E. Tallei Assessment of Genetic Variation in Apis nigrocincta (Hymenoptera: Apidae) in Sulawesi Revealed by Partial Mitochondrial Cytochrome Oxidase I Gene Sequences Scientifica |
| title | Assessment of Genetic Variation in Apis nigrocincta (Hymenoptera: Apidae) in Sulawesi Revealed by Partial Mitochondrial Cytochrome Oxidase I Gene Sequences |
| title_full | Assessment of Genetic Variation in Apis nigrocincta (Hymenoptera: Apidae) in Sulawesi Revealed by Partial Mitochondrial Cytochrome Oxidase I Gene Sequences |
| title_fullStr | Assessment of Genetic Variation in Apis nigrocincta (Hymenoptera: Apidae) in Sulawesi Revealed by Partial Mitochondrial Cytochrome Oxidase I Gene Sequences |
| title_full_unstemmed | Assessment of Genetic Variation in Apis nigrocincta (Hymenoptera: Apidae) in Sulawesi Revealed by Partial Mitochondrial Cytochrome Oxidase I Gene Sequences |
| title_short | Assessment of Genetic Variation in Apis nigrocincta (Hymenoptera: Apidae) in Sulawesi Revealed by Partial Mitochondrial Cytochrome Oxidase I Gene Sequences |
| title_sort | assessment of genetic variation in apis nigrocincta hymenoptera apidae in sulawesi revealed by partial mitochondrial cytochrome oxidase i gene sequences |
| url | http://dx.doi.org/10.1155/2020/1609473 |
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