Reconstructing Three‐Dimensional Optical Anisotropy with Tomographic Müller‐Polarimetric Microscopy
Abstract Most visible light imaging methods using polarization to obtain ultrastructure information are limited to 2D analysis or require demanding phase measurements to be extended to 3D. A novel 3D polarized light imaging technique based on Müller‐matrix formulations is introduced which numericall...
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| Format: | Article |
| Language: | English |
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Wiley
2025-07-01
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| Series: | Advanced Science |
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| Online Access: | https://doi.org/10.1002/advs.202502075 |
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| author | Yang Chen Arthur Baroni Torne Tänzer Leonard Nielsen Marianne Liebi |
| author_facet | Yang Chen Arthur Baroni Torne Tänzer Leonard Nielsen Marianne Liebi |
| author_sort | Yang Chen |
| collection | DOAJ |
| description | Abstract Most visible light imaging methods using polarization to obtain ultrastructure information are limited to 2D analysis or require demanding phase measurements to be extended to 3D. A novel 3D polarized light imaging technique based on Müller‐matrix formulations is introduced which numerically reconstructs 3D optical birefringence, that is anisotropic refractive indices and optical axis orientation, in each volumetric unit of sample. The new method is demonstrated, tomographic Müller‐polarimetric microscopy, in simulation and using experimental data of 3D macroscopic sample of human trabecular bone sample, where the local main orientation of nanoscale collagen fibers is extracted with a resolution of ≈ 20 µm. Tomographic Müller‐polarimetric microscopy offers a low‐cost and experimentally simple imaging approach to access the ultrastructure which is not directly resolvable, in a wide range of biological and composite materials. |
| format | Article |
| id | doaj-art-edb2bafbe39c4b8b81ab16e01694131c |
| institution | Kabale University |
| issn | 2198-3844 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advanced Science |
| spelling | doaj-art-edb2bafbe39c4b8b81ab16e01694131c2025-08-20T03:50:58ZengWileyAdvanced Science2198-38442025-07-011227n/an/a10.1002/advs.202502075Reconstructing Three‐Dimensional Optical Anisotropy with Tomographic Müller‐Polarimetric MicroscopyYang Chen0Arthur Baroni1Torne Tänzer2Leonard Nielsen3Marianne Liebi4Center for Photon SciencePaul Scherrer InstitutVilligen PSI 5232 SwitzerlandCenter for Photon SciencePaul Scherrer InstitutVilligen PSI 5232 SwitzerlandCenter for Photon SciencePaul Scherrer InstitutVilligen PSI 5232 SwitzerlandDepartment of PhysicsChalmers University of TechnologyGothenburg41296SwedenCenter for Photon SciencePaul Scherrer InstitutVilligen PSI 5232 SwitzerlandAbstract Most visible light imaging methods using polarization to obtain ultrastructure information are limited to 2D analysis or require demanding phase measurements to be extended to 3D. A novel 3D polarized light imaging technique based on Müller‐matrix formulations is introduced which numerically reconstructs 3D optical birefringence, that is anisotropic refractive indices and optical axis orientation, in each volumetric unit of sample. The new method is demonstrated, tomographic Müller‐polarimetric microscopy, in simulation and using experimental data of 3D macroscopic sample of human trabecular bone sample, where the local main orientation of nanoscale collagen fibers is extracted with a resolution of ≈ 20 µm. Tomographic Müller‐polarimetric microscopy offers a low‐cost and experimentally simple imaging approach to access the ultrastructure which is not directly resolvable, in a wide range of biological and composite materials.https://doi.org/10.1002/advs.2025020753D ultrastructurebio‐imaginghuman trabecular bonereconstructiontomographic polarized light microscopy |
| spellingShingle | Yang Chen Arthur Baroni Torne Tänzer Leonard Nielsen Marianne Liebi Reconstructing Three‐Dimensional Optical Anisotropy with Tomographic Müller‐Polarimetric Microscopy Advanced Science 3D ultrastructure bio‐imaging human trabecular bone reconstruction tomographic polarized light microscopy |
| title | Reconstructing Three‐Dimensional Optical Anisotropy with Tomographic Müller‐Polarimetric Microscopy |
| title_full | Reconstructing Three‐Dimensional Optical Anisotropy with Tomographic Müller‐Polarimetric Microscopy |
| title_fullStr | Reconstructing Three‐Dimensional Optical Anisotropy with Tomographic Müller‐Polarimetric Microscopy |
| title_full_unstemmed | Reconstructing Three‐Dimensional Optical Anisotropy with Tomographic Müller‐Polarimetric Microscopy |
| title_short | Reconstructing Three‐Dimensional Optical Anisotropy with Tomographic Müller‐Polarimetric Microscopy |
| title_sort | reconstructing three dimensional optical anisotropy with tomographic muller polarimetric microscopy |
| topic | 3D ultrastructure bio‐imaging human trabecular bone reconstruction tomographic polarized light microscopy |
| url | https://doi.org/10.1002/advs.202502075 |
| work_keys_str_mv | AT yangchen reconstructingthreedimensionalopticalanisotropywithtomographicmullerpolarimetricmicroscopy AT arthurbaroni reconstructingthreedimensionalopticalanisotropywithtomographicmullerpolarimetricmicroscopy AT tornetanzer reconstructingthreedimensionalopticalanisotropywithtomographicmullerpolarimetricmicroscopy AT leonardnielsen reconstructingthreedimensionalopticalanisotropywithtomographicmullerpolarimetricmicroscopy AT marianneliebi reconstructingthreedimensionalopticalanisotropywithtomographicmullerpolarimetricmicroscopy |