Analysis of Fatty Acid Profile, α-Tocopherol, Squalene and Cholesterol Content in Edible Parts and By-Products of South Pacific Wild Fishes

Fish are generally rich sources of n-3 polyunsaturated fatty acids such as EPA and DHA, and although the edible part (fillet) has been analyzed in many species, less is known about the composition of fish by-products. The analysis of these materials allows them to be evaluated as raw sources of EPA...

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Main Authors: Sussi López-Puebla, María Fernanda Arias-Santé, Jaime Romero, Adriano Costa de Camargo, Miguel Ángel Rincón-Cervera
Format: Article
Language:English
Published: MDPI AG 2025-02-01
Series:Marine Drugs
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Online Access:https://www.mdpi.com/1660-3397/23/3/104
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author Sussi López-Puebla
María Fernanda Arias-Santé
Jaime Romero
Adriano Costa de Camargo
Miguel Ángel Rincón-Cervera
author_facet Sussi López-Puebla
María Fernanda Arias-Santé
Jaime Romero
Adriano Costa de Camargo
Miguel Ángel Rincón-Cervera
author_sort Sussi López-Puebla
collection DOAJ
description Fish are generally rich sources of n-3 polyunsaturated fatty acids such as EPA and DHA, and although the edible part (fillet) has been analyzed in many species, less is known about the composition of fish by-products. The analysis of these materials allows them to be evaluated as raw sources of EPA and DHA, thus contributing to sustainable practices to produce healthy oils for human consumption. This work provides information on the fatty acid profiles, lipid quality indices and α-tocopherol, squalene and cholesterol contents in fillets, heads, bones and viscera of three fish species (anchovy, chub mackerel and Chilean jack mackerel). Samples were lyophilized and lipids were extracted using either the Folch or Hara and Radin methods. FA profiles were obtained by gas chromatography coupled with flame ionization detection, and tocopherol, squalene and cholesterol analyses were performed by high performance liquid chromatography with UV-visible detection. The highest levels of EPA were found in anchovy fillet (18.9–20.6%) and bone (14.7%), while DHA was more abundant in anchovy fillet (16.9–22.0%) and Chilean jack mackerel fillet (15.4–16.6%) and bone (13.1–13.8%). α-Tocopherol, squalene and cholesterol contents ranged from 0.18 to 1.35 mg/100 g, 0.07 to 0.80 mg/100 g and 30.46 to 246.17 mg/100 g, respectively, in the different tissues analyzed from the three fish species.
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spelling doaj-art-edb268ae6ba649e09357e1711bd2aaee2025-08-20T01:48:52ZengMDPI AGMarine Drugs1660-33972025-02-0123310410.3390/md23030104Analysis of Fatty Acid Profile, α-Tocopherol, Squalene and Cholesterol Content in Edible Parts and By-Products of South Pacific Wild FishesSussi López-Puebla0María Fernanda Arias-Santé1Jaime Romero2Adriano Costa de Camargo3Miguel Ángel Rincón-Cervera4Institute of Nutrition and Food Technology, University of Chile, Macul, Santiago 7830490, ChileInstitute of Nutrition and Food Technology, University of Chile, Macul, Santiago 7830490, ChileInstitute of Nutrition and Food Technology, University of Chile, Macul, Santiago 7830490, ChileInstitute of Nutrition and Food Technology, University of Chile, Macul, Santiago 7830490, ChileInstitute of Nutrition and Food Technology, University of Chile, Macul, Santiago 7830490, ChileFish are generally rich sources of n-3 polyunsaturated fatty acids such as EPA and DHA, and although the edible part (fillet) has been analyzed in many species, less is known about the composition of fish by-products. The analysis of these materials allows them to be evaluated as raw sources of EPA and DHA, thus contributing to sustainable practices to produce healthy oils for human consumption. This work provides information on the fatty acid profiles, lipid quality indices and α-tocopherol, squalene and cholesterol contents in fillets, heads, bones and viscera of three fish species (anchovy, chub mackerel and Chilean jack mackerel). Samples were lyophilized and lipids were extracted using either the Folch or Hara and Radin methods. FA profiles were obtained by gas chromatography coupled with flame ionization detection, and tocopherol, squalene and cholesterol analyses were performed by high performance liquid chromatography with UV-visible detection. The highest levels of EPA were found in anchovy fillet (18.9–20.6%) and bone (14.7%), while DHA was more abundant in anchovy fillet (16.9–22.0%) and Chilean jack mackerel fillet (15.4–16.6%) and bone (13.1–13.8%). α-Tocopherol, squalene and cholesterol contents ranged from 0.18 to 1.35 mg/100 g, 0.07 to 0.80 mg/100 g and 30.46 to 246.17 mg/100 g, respectively, in the different tissues analyzed from the three fish species.https://www.mdpi.com/1660-3397/23/3/104fishby-productsomega-3 fatty acidstocopherolsqualenecholesterol
spellingShingle Sussi López-Puebla
María Fernanda Arias-Santé
Jaime Romero
Adriano Costa de Camargo
Miguel Ángel Rincón-Cervera
Analysis of Fatty Acid Profile, α-Tocopherol, Squalene and Cholesterol Content in Edible Parts and By-Products of South Pacific Wild Fishes
Marine Drugs
fish
by-products
omega-3 fatty acids
tocopherol
squalene
cholesterol
title Analysis of Fatty Acid Profile, α-Tocopherol, Squalene and Cholesterol Content in Edible Parts and By-Products of South Pacific Wild Fishes
title_full Analysis of Fatty Acid Profile, α-Tocopherol, Squalene and Cholesterol Content in Edible Parts and By-Products of South Pacific Wild Fishes
title_fullStr Analysis of Fatty Acid Profile, α-Tocopherol, Squalene and Cholesterol Content in Edible Parts and By-Products of South Pacific Wild Fishes
title_full_unstemmed Analysis of Fatty Acid Profile, α-Tocopherol, Squalene and Cholesterol Content in Edible Parts and By-Products of South Pacific Wild Fishes
title_short Analysis of Fatty Acid Profile, α-Tocopherol, Squalene and Cholesterol Content in Edible Parts and By-Products of South Pacific Wild Fishes
title_sort analysis of fatty acid profile α tocopherol squalene and cholesterol content in edible parts and by products of south pacific wild fishes
topic fish
by-products
omega-3 fatty acids
tocopherol
squalene
cholesterol
url https://www.mdpi.com/1660-3397/23/3/104
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