Advantages, applications, and future directions of in vivo aptamer SELEX: A review
In vivo systematic evolution of ligands by exponential enrichment (SELEX) emerged as a transformative technique for directly identifying aptamers within living organisms. Unlike traditional in vitro methods that often fail to capture the complexity of biological interactions in physiological environ...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-09-01
|
| Series: | Molecular Therapy: Nucleic Acids |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2162253125001295 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849726078277386240 |
|---|---|
| author | Minchuan Lyu Chi Ho Chan Ziqi Chen Yumeng Liu Yuanyuan Yu |
| author_facet | Minchuan Lyu Chi Ho Chan Ziqi Chen Yumeng Liu Yuanyuan Yu |
| author_sort | Minchuan Lyu |
| collection | DOAJ |
| description | In vivo systematic evolution of ligands by exponential enrichment (SELEX) emerged as a transformative technique for directly identifying aptamers within living organisms. Unlike traditional in vitro methods that often fail to capture the complexity of biological interactions in physiological environments, in vivo SELEX leverages whole living organisms as selection targets. This strategy significantly enhances the specificity, functionality, and physiological relevance of the selected aptamers, making them more viable for therapeutic, diagnostic, and imaging applications. The aim of this review is to elucidate the unique advantages of in vivo SELEX compared to conventional in vitro techniques. It focuses on how in vivo SELEX improves aptamer specificity, physiological relevance, and pharmacokinetic properties, thereby enhancing their potential for use in diagnostics and therapeutics. This review highlights the principles, advancements, and challenges of in vivo SELEX, while discussing its potential to bridge the gap between aptamer discovery and clinical translation. By enabling the selection of aptamers under real physiological conditions, in vivo SELEX addresses critical limitations of in vitro methods, such as off-target effects and poor clinical translatability. The review also explores the applications of in vivo SELEX in various fields, including neurology, oncology, and cardiovascular diseases, and provides insights into future directions for optimizing this technology. |
| format | Article |
| id | doaj-art-edacebbedc684ef88d6ee2c0d6107e88 |
| institution | DOAJ |
| issn | 2162-2531 |
| language | English |
| publishDate | 2025-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Molecular Therapy: Nucleic Acids |
| spelling | doaj-art-edacebbedc684ef88d6ee2c0d6107e882025-08-20T03:10:18ZengElsevierMolecular Therapy: Nucleic Acids2162-25312025-09-0136310257510.1016/j.omtn.2025.102575Advantages, applications, and future directions of in vivo aptamer SELEX: A reviewMinchuan Lyu0Chi Ho Chan1Ziqi Chen2Yumeng Liu3Yuanyuan Yu4Institute of Integrated Bioinformedicine and Translational Science (IBTS), School of Chinese Medicine, Hong Kong Baptist University, Hong Kong SAR 000000, China; Institute of Systems Medicine and Health Sciences (SMHS), School of Chinese Medicine, Hong Kong Baptist University, Hong Kong SAR 000000, China; Guangdong-Hong Kong-Macao Greater Bay Area International Research Platform for Aptamer-based Translational Medicine and Drug Discovery, Hong Kong SAR 000000, ChinaInstitute of Integrated Bioinformedicine and Translational Science (IBTS), School of Chinese Medicine, Hong Kong Baptist University, Hong Kong SAR 000000, China; Institute of Systems Medicine and Health Sciences (SMHS), School of Chinese Medicine, Hong Kong Baptist University, Hong Kong SAR 000000, China; Guangdong-Hong Kong-Macao Greater Bay Area International Research Platform for Aptamer-based Translational Medicine and Drug Discovery, Hong Kong SAR 000000, ChinaInstitute of Integrated Bioinformedicine and Translational Science (IBTS), School of Chinese Medicine, Hong Kong Baptist University, Hong Kong SAR 000000, China; Institute of Systems Medicine and Health Sciences (SMHS), School of Chinese Medicine, Hong Kong Baptist University, Hong Kong SAR 000000, China; Guangdong-Hong Kong-Macao Greater Bay Area International Research Platform for Aptamer-based Translational Medicine and Drug Discovery, Hong Kong SAR 000000, ChinaInstitute of Integrated Bioinformedicine and Translational Science (IBTS), School of Chinese Medicine, Hong Kong Baptist University, Hong Kong SAR 000000, China; Institute of Systems Medicine and Health Sciences (SMHS), School of Chinese Medicine, Hong Kong Baptist University, Hong Kong SAR 000000, China; Guangdong-Hong Kong-Macao Greater Bay Area International Research Platform for Aptamer-based Translational Medicine and Drug Discovery, Hong Kong SAR 000000, ChinaInstitute of Integrated Bioinformedicine and Translational Science (IBTS), School of Chinese Medicine, Hong Kong Baptist University, Hong Kong SAR 000000, China; Institute of Systems Medicine and Health Sciences (SMHS), School of Chinese Medicine, Hong Kong Baptist University, Hong Kong SAR 000000, China; Guangdong-Hong Kong-Macao Greater Bay Area International Research Platform for Aptamer-based Translational Medicine and Drug Discovery, Hong Kong SAR 000000, China; Corresponding author: Yuanyuan Yu, Institute of Integrated Bioinformedicine and Translational Science (IBTS), School of Chinese Medicine, Hong Kong Baptist University, Hong Kong SAR 000000, China.In vivo systematic evolution of ligands by exponential enrichment (SELEX) emerged as a transformative technique for directly identifying aptamers within living organisms. Unlike traditional in vitro methods that often fail to capture the complexity of biological interactions in physiological environments, in vivo SELEX leverages whole living organisms as selection targets. This strategy significantly enhances the specificity, functionality, and physiological relevance of the selected aptamers, making them more viable for therapeutic, diagnostic, and imaging applications. The aim of this review is to elucidate the unique advantages of in vivo SELEX compared to conventional in vitro techniques. It focuses on how in vivo SELEX improves aptamer specificity, physiological relevance, and pharmacokinetic properties, thereby enhancing their potential for use in diagnostics and therapeutics. This review highlights the principles, advancements, and challenges of in vivo SELEX, while discussing its potential to bridge the gap between aptamer discovery and clinical translation. By enabling the selection of aptamers under real physiological conditions, in vivo SELEX addresses critical limitations of in vitro methods, such as off-target effects and poor clinical translatability. The review also explores the applications of in vivo SELEX in various fields, including neurology, oncology, and cardiovascular diseases, and provides insights into future directions for optimizing this technology.http://www.sciencedirect.com/science/article/pii/S2162253125001295MT: Oligonucleotides: Therapies and Applicationsaptamersin vivo SELEXin vitro SELEX |
| spellingShingle | Minchuan Lyu Chi Ho Chan Ziqi Chen Yumeng Liu Yuanyuan Yu Advantages, applications, and future directions of in vivo aptamer SELEX: A review Molecular Therapy: Nucleic Acids MT: Oligonucleotides: Therapies and Applications aptamers in vivo SELEX in vitro SELEX |
| title | Advantages, applications, and future directions of in vivo aptamer SELEX: A review |
| title_full | Advantages, applications, and future directions of in vivo aptamer SELEX: A review |
| title_fullStr | Advantages, applications, and future directions of in vivo aptamer SELEX: A review |
| title_full_unstemmed | Advantages, applications, and future directions of in vivo aptamer SELEX: A review |
| title_short | Advantages, applications, and future directions of in vivo aptamer SELEX: A review |
| title_sort | advantages applications and future directions of in vivo aptamer selex a review |
| topic | MT: Oligonucleotides: Therapies and Applications aptamers in vivo SELEX in vitro SELEX |
| url | http://www.sciencedirect.com/science/article/pii/S2162253125001295 |
| work_keys_str_mv | AT minchuanlyu advantagesapplicationsandfuturedirectionsofinvivoaptamerselexareview AT chihochan advantagesapplicationsandfuturedirectionsofinvivoaptamerselexareview AT ziqichen advantagesapplicationsandfuturedirectionsofinvivoaptamerselexareview AT yumengliu advantagesapplicationsandfuturedirectionsofinvivoaptamerselexareview AT yuanyuanyu advantagesapplicationsandfuturedirectionsofinvivoaptamerselexareview |