Histopathological characteristics of PRRS and expression profiles of viral receptors in the piglet immune system
Porcine reproductive and respiratory syndrome (PRRS) is a highly contagious viral disease that causes significant economic losses to the swine industry worldwide. PRRS virus (PRRSV) infection is a receptor-mediated endocytosis and replication process. The purpose of this study was to determine the l...
Saved in:
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2024-11-01
|
| Series: | Frontiers in Veterinary Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2024.1428273/full |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850199941041881088 |
|---|---|
| author | Hong Chen Na Chen Hongbo Chen Zefang Zhao Jiayao Yang Jianbo Sun Hanmei Li Rihua Cong Hailong Liu Tengfei Liu Shulin Chen |
| author_facet | Hong Chen Na Chen Hongbo Chen Zefang Zhao Jiayao Yang Jianbo Sun Hanmei Li Rihua Cong Hailong Liu Tengfei Liu Shulin Chen |
| author_sort | Hong Chen |
| collection | DOAJ |
| description | Porcine reproductive and respiratory syndrome (PRRS) is a highly contagious viral disease that causes significant economic losses to the swine industry worldwide. PRRS virus (PRRSV) infection is a receptor-mediated endocytosis and replication process. The purpose of this study was to determine the localization and expression of four important PRRSV receptors in immunological organs of piglets. After piglets were infected with PRRSV, Hematoxylin and Eosin staining, immunofluorescence, and Western blot were used to perform histopathological examination and receptors distribution analysis. The results showed that PRRSV caused severe damage to the piglets’ immune organs, including atrophy of the thymus and swelling of lymph node. Histopathological lesions were mainly observed in the lung and lymph node and were characterized by interstitial pneumonia, collapsed follicles, exhaustion of germinal centers, and extensive hemorrhage. Immunofluorescence staining and Western blot results showed that the receptors of CD163 and NMHCII-A were mainly distributed in the thymus, hilar lymph nodes, and mesenteric lymph nodes. However, Sn and vimentin receptors were expressed at low levels in the immune organs of piglets. The distribution of the four receptors in the immune organs was more concentrated in the cortex but was more scattered in the medulla. Compared to the control group, the relative expression of the four receptors increased significantly in most immune organs after viral infection. In conclusion, our study examined the distribution and expression of four PRRSV receptors in immunological organs. We observed a significant increase in the expression of Sn, CD163, and vimentin following viral infection. These findings may provide potential targets for future antiviral reagent design or vaccine development. |
| format | Article |
| id | doaj-art-edaad74273b64687a9b7b8eeb0ce0a69 |
| institution | OA Journals |
| issn | 2297-1769 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Veterinary Science |
| spelling | doaj-art-edaad74273b64687a9b7b8eeb0ce0a692025-08-20T02:12:29ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692024-11-011110.3389/fvets.2024.14282731428273Histopathological characteristics of PRRS and expression profiles of viral receptors in the piglet immune systemHong Chen0Na Chen1Hongbo Chen2Zefang Zhao3Jiayao Yang4Jianbo Sun5Hanmei Li6Rihua Cong7Hailong Liu8Tengfei Liu9Shulin Chen10Department of Basic Veterinary Medicine, College of Veterinary Medicine, Northwest A&F University, Yangling, ChinaDepartment of Basic Veterinary Medicine, College of Veterinary Medicine, Northwest A&F University, Yangling, ChinaDepartment of Basic Veterinary Medicine, College of Veterinary Medicine, Northwest A&F University, Yangling, ChinaDepartment of Basic Veterinary Medicine, College of Veterinary Medicine, Northwest A&F University, Yangling, ChinaDepartment of Basic Veterinary Medicine, College of Veterinary Medicine, Northwest A&F University, Yangling, ChinaDepartment of Basic Veterinary Medicine, College of Veterinary Medicine, Northwest A&F University, Yangling, ChinaDepartment of Basic Veterinary Medicine, College of Veterinary Medicine, Northwest A&F University, Yangling, ChinaDepartment of Basic Veterinary Medicine, College of Veterinary Medicine, Northwest A&F University, Yangling, ChinaInstitute of Animal Science and Veterinary Medicine, Hainan Academy of Agricultural Sciences, Haikou, ChinaDepartment of Basic Veterinary Medicine, College of Veterinary Medicine, Northwest A&F University, Yangling, ChinaDepartment of Basic Veterinary Medicine, College of Veterinary Medicine, Northwest A&F University, Yangling, ChinaPorcine reproductive and respiratory syndrome (PRRS) is a highly contagious viral disease that causes significant economic losses to the swine industry worldwide. PRRS virus (PRRSV) infection is a receptor-mediated endocytosis and replication process. The purpose of this study was to determine the localization and expression of four important PRRSV receptors in immunological organs of piglets. After piglets were infected with PRRSV, Hematoxylin and Eosin staining, immunofluorescence, and Western blot were used to perform histopathological examination and receptors distribution analysis. The results showed that PRRSV caused severe damage to the piglets’ immune organs, including atrophy of the thymus and swelling of lymph node. Histopathological lesions were mainly observed in the lung and lymph node and were characterized by interstitial pneumonia, collapsed follicles, exhaustion of germinal centers, and extensive hemorrhage. Immunofluorescence staining and Western blot results showed that the receptors of CD163 and NMHCII-A were mainly distributed in the thymus, hilar lymph nodes, and mesenteric lymph nodes. However, Sn and vimentin receptors were expressed at low levels in the immune organs of piglets. The distribution of the four receptors in the immune organs was more concentrated in the cortex but was more scattered in the medulla. Compared to the control group, the relative expression of the four receptors increased significantly in most immune organs after viral infection. In conclusion, our study examined the distribution and expression of four PRRSV receptors in immunological organs. We observed a significant increase in the expression of Sn, CD163, and vimentin following viral infection. These findings may provide potential targets for future antiviral reagent design or vaccine development.https://www.frontiersin.org/articles/10.3389/fvets.2024.1428273/fullPRRSVimmune organsviral receptorshistopathologypiglets |
| spellingShingle | Hong Chen Na Chen Hongbo Chen Zefang Zhao Jiayao Yang Jianbo Sun Hanmei Li Rihua Cong Hailong Liu Tengfei Liu Shulin Chen Histopathological characteristics of PRRS and expression profiles of viral receptors in the piglet immune system Frontiers in Veterinary Science PRRSV immune organs viral receptors histopathology piglets |
| title | Histopathological characteristics of PRRS and expression profiles of viral receptors in the piglet immune system |
| title_full | Histopathological characteristics of PRRS and expression profiles of viral receptors in the piglet immune system |
| title_fullStr | Histopathological characteristics of PRRS and expression profiles of viral receptors in the piglet immune system |
| title_full_unstemmed | Histopathological characteristics of PRRS and expression profiles of viral receptors in the piglet immune system |
| title_short | Histopathological characteristics of PRRS and expression profiles of viral receptors in the piglet immune system |
| title_sort | histopathological characteristics of prrs and expression profiles of viral receptors in the piglet immune system |
| topic | PRRSV immune organs viral receptors histopathology piglets |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2024.1428273/full |
| work_keys_str_mv | AT hongchen histopathologicalcharacteristicsofprrsandexpressionprofilesofviralreceptorsinthepigletimmunesystem AT nachen histopathologicalcharacteristicsofprrsandexpressionprofilesofviralreceptorsinthepigletimmunesystem AT hongbochen histopathologicalcharacteristicsofprrsandexpressionprofilesofviralreceptorsinthepigletimmunesystem AT zefangzhao histopathologicalcharacteristicsofprrsandexpressionprofilesofviralreceptorsinthepigletimmunesystem AT jiayaoyang histopathologicalcharacteristicsofprrsandexpressionprofilesofviralreceptorsinthepigletimmunesystem AT jianbosun histopathologicalcharacteristicsofprrsandexpressionprofilesofviralreceptorsinthepigletimmunesystem AT hanmeili histopathologicalcharacteristicsofprrsandexpressionprofilesofviralreceptorsinthepigletimmunesystem AT rihuacong histopathologicalcharacteristicsofprrsandexpressionprofilesofviralreceptorsinthepigletimmunesystem AT hailongliu histopathologicalcharacteristicsofprrsandexpressionprofilesofviralreceptorsinthepigletimmunesystem AT tengfeiliu histopathologicalcharacteristicsofprrsandexpressionprofilesofviralreceptorsinthepigletimmunesystem AT shulinchen histopathologicalcharacteristicsofprrsandexpressionprofilesofviralreceptorsinthepigletimmunesystem |