Particulate multivalent presentation of the receptor binding domain induces protective immune responses against MERS-CoV
Middle East respiratory syndrome coronavirus (MERS-CoV) is a WHO priority pathogen for which vaccines are urgently needed. Using an immune-focusing approach, we created self-assembling particles multivalently displaying critical regions of the MERS-CoV spike protein ─fusion peptide, heptad repeat 2,...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2020-01-01
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| Series: | Emerging Microbes and Infections |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/22221751.2020.1760735 |
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| author | Nisreen M. A. Okba Ivy Widjaja Brenda van Dieren Andrea Aebischer Geert van Amerongen Leon de Waal Koert J. Stittelaar Debby Schipper Byron Martina Judith M. A. van den Brand Martin Beer Berend-Jan Bosch Bart L. Haagmans |
| author_facet | Nisreen M. A. Okba Ivy Widjaja Brenda van Dieren Andrea Aebischer Geert van Amerongen Leon de Waal Koert J. Stittelaar Debby Schipper Byron Martina Judith M. A. van den Brand Martin Beer Berend-Jan Bosch Bart L. Haagmans |
| author_sort | Nisreen M. A. Okba |
| collection | DOAJ |
| description | Middle East respiratory syndrome coronavirus (MERS-CoV) is a WHO priority pathogen for which vaccines are urgently needed. Using an immune-focusing approach, we created self-assembling particles multivalently displaying critical regions of the MERS-CoV spike protein ─fusion peptide, heptad repeat 2, and receptor binding domain (RBD) ─ and tested their immunogenicity and protective capacity in rabbits. Using a “plug-and-display” SpyTag/SpyCatcher system, we coupled RBD to lumazine synthase (LS) particles producing multimeric RBD-presenting particles (RBD-LS). RBD-LS vaccination induced antibody responses of high magnitude and quality (avidity, MERS-CoV neutralizing capacity, and mucosal immunity) with cross-clade neutralization. The antibody responses were associated with blocking viral replication and upper and lower respiratory tract protection against MERS-CoV infection in rabbits. This arrayed multivalent presentation of the viral RBD using the antigen-SpyTag/LS-SpyCatcher is a promising MERS-CoV vaccine candidate and this platform may be applied for the rapid development of vaccines against other emerging viruses such as SARS-CoV-2. |
| format | Article |
| id | doaj-art-eda04ba2d36a4ed8b9a203977286ddba |
| institution | DOAJ |
| issn | 2222-1751 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Emerging Microbes and Infections |
| spelling | doaj-art-eda04ba2d36a4ed8b9a203977286ddba2025-08-20T03:08:31ZengTaylor & Francis GroupEmerging Microbes and Infections2222-17512020-01-01911080109110.1080/22221751.2020.1760735Particulate multivalent presentation of the receptor binding domain induces protective immune responses against MERS-CoVNisreen M. A. Okba0Ivy Widjaja1Brenda van Dieren2Andrea Aebischer3Geert van Amerongen4Leon de Waal5Koert J. Stittelaar6Debby Schipper7Byron Martina8Judith M. A. van den Brand9Martin Beer10Berend-Jan Bosch11Bart L. Haagmans12Department of Viroscience, Erasmus Medical Center, Rotterdam, The NetherlandsVirology Division, Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The NetherlandsVirology Division, Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The NetherlandsInstitute of Diagnostic Virology, Friedrich-Loeffler-Institut, Insel Riems, GermanyViroclinics Biosciences BV, Rotterdam, The NetherlandsViroclinics Biosciences BV, Rotterdam, The NetherlandsViroclinics Biosciences BV, Rotterdam, The NetherlandsDepartment of Viroscience, Erasmus Medical Center, Rotterdam, The NetherlandsDepartment of Viroscience, Erasmus Medical Center, Rotterdam, The NetherlandsDepartment of Viroscience, Erasmus Medical Center, Rotterdam, The NetherlandsInstitute of Diagnostic Virology, Friedrich-Loeffler-Institut, Insel Riems, GermanyVirology Division, Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The NetherlandsDepartment of Viroscience, Erasmus Medical Center, Rotterdam, The NetherlandsMiddle East respiratory syndrome coronavirus (MERS-CoV) is a WHO priority pathogen for which vaccines are urgently needed. Using an immune-focusing approach, we created self-assembling particles multivalently displaying critical regions of the MERS-CoV spike protein ─fusion peptide, heptad repeat 2, and receptor binding domain (RBD) ─ and tested their immunogenicity and protective capacity in rabbits. Using a “plug-and-display” SpyTag/SpyCatcher system, we coupled RBD to lumazine synthase (LS) particles producing multimeric RBD-presenting particles (RBD-LS). RBD-LS vaccination induced antibody responses of high magnitude and quality (avidity, MERS-CoV neutralizing capacity, and mucosal immunity) with cross-clade neutralization. The antibody responses were associated with blocking viral replication and upper and lower respiratory tract protection against MERS-CoV infection in rabbits. This arrayed multivalent presentation of the viral RBD using the antigen-SpyTag/LS-SpyCatcher is a promising MERS-CoV vaccine candidate and this platform may be applied for the rapid development of vaccines against other emerging viruses such as SARS-CoV-2.https://www.tandfonline.com/doi/10.1080/22221751.2020.1760735VaccineMERS-coronavirusspikei301lumazine synthasespytag-spycatcher |
| spellingShingle | Nisreen M. A. Okba Ivy Widjaja Brenda van Dieren Andrea Aebischer Geert van Amerongen Leon de Waal Koert J. Stittelaar Debby Schipper Byron Martina Judith M. A. van den Brand Martin Beer Berend-Jan Bosch Bart L. Haagmans Particulate multivalent presentation of the receptor binding domain induces protective immune responses against MERS-CoV Emerging Microbes and Infections Vaccine MERS-coronavirus spike i301 lumazine synthase spytag-spycatcher |
| title | Particulate multivalent presentation of the receptor binding domain induces protective immune responses against MERS-CoV |
| title_full | Particulate multivalent presentation of the receptor binding domain induces protective immune responses against MERS-CoV |
| title_fullStr | Particulate multivalent presentation of the receptor binding domain induces protective immune responses against MERS-CoV |
| title_full_unstemmed | Particulate multivalent presentation of the receptor binding domain induces protective immune responses against MERS-CoV |
| title_short | Particulate multivalent presentation of the receptor binding domain induces protective immune responses against MERS-CoV |
| title_sort | particulate multivalent presentation of the receptor binding domain induces protective immune responses against mers cov |
| topic | Vaccine MERS-coronavirus spike i301 lumazine synthase spytag-spycatcher |
| url | https://www.tandfonline.com/doi/10.1080/22221751.2020.1760735 |
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