DNA vaccination induced protective immunity against SARS CoV-2 infection in hamsterss.
The development of efficient vaccines against COVID-19 is an emergent need for global public health. The spike protein of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a major target for the COVID-19 vaccine. To quickly respond to the outbreak of the SARS-CoV-2 pandemic, a nucleic...
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Public Library of Science (PLoS)
2021-05-01
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| Series: | PLoS Neglected Tropical Diseases |
| Online Access: | https://journals.plos.org/plosntds/article/file?id=10.1371/journal.pntd.0009374&type=printable |
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| author | Kit Man Chai Tsai-Teng Tzeng Kuan-Yin Shen Hung-Chun Liao Jhe-Jhih Lin Mei-Yu Chen Guann-Yi Yu Horng-Yunn Dou Ching-Len Liao Hsin-Wei Chen Shih-Jen Liu |
| author_facet | Kit Man Chai Tsai-Teng Tzeng Kuan-Yin Shen Hung-Chun Liao Jhe-Jhih Lin Mei-Yu Chen Guann-Yi Yu Horng-Yunn Dou Ching-Len Liao Hsin-Wei Chen Shih-Jen Liu |
| author_sort | Kit Man Chai |
| collection | DOAJ |
| description | The development of efficient vaccines against COVID-19 is an emergent need for global public health. The spike protein of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a major target for the COVID-19 vaccine. To quickly respond to the outbreak of the SARS-CoV-2 pandemic, a nucleic acid-based vaccine is a novel option, beyond the traditional inactivated virus vaccine or recombinant protein vaccine. Here, we report a DNA vaccine containing the spike gene for delivery via electroporation. The spike genes of SARS-CoV and SARS-CoV-2 were codon optimized for mammalian cell expression and then cloned into mammalian cell expression vectors, called pSARS-S and pSARS2-S, respectively. Spike protein expression was confirmed by immunoblotting after transient expression in HEK293T cells. After immunization, sera were collected for antigen-specific antibody and neutralizing antibody titer analyses. We found that both pSARS-S and pSARS2-S immunization induced similar levels of antibodies against S2 of SARS-CoV-2. In contrast, only pSARS2-S immunization induced antibodies against the receptor-binding domain of SARS-CoV-2. We further found that pSARS2-S immunization, but not pSARS-S immunization, could induce very high titers of neutralizing antibodies against SARS-CoV-2. We further analyzed SARS-CoV-2 S protein-specific T cell responses and found that the immune responses were biased toward Th1. Importantly, pSARS2-S immunization in hamsters could induce protective immunity against SARS-CoV-2 challenge in vivo. These data suggest that DNA vaccination could be a promising approach for protecting against COVID-19. |
| format | Article |
| id | doaj-art-ed966694d2fb48dfa78c64314db836a2 |
| institution | DOAJ |
| issn | 1935-2727 1935-2735 |
| language | English |
| publishDate | 2021-05-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Neglected Tropical Diseases |
| spelling | doaj-art-ed966694d2fb48dfa78c64314db836a22025-08-20T02:54:29ZengPublic Library of Science (PLoS)PLoS Neglected Tropical Diseases1935-27271935-27352021-05-01155e000937410.1371/journal.pntd.0009374DNA vaccination induced protective immunity against SARS CoV-2 infection in hamsterss.Kit Man ChaiTsai-Teng TzengKuan-Yin ShenHung-Chun LiaoJhe-Jhih LinMei-Yu ChenGuann-Yi YuHorng-Yunn DouChing-Len LiaoHsin-Wei ChenShih-Jen LiuThe development of efficient vaccines against COVID-19 is an emergent need for global public health. The spike protein of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a major target for the COVID-19 vaccine. To quickly respond to the outbreak of the SARS-CoV-2 pandemic, a nucleic acid-based vaccine is a novel option, beyond the traditional inactivated virus vaccine or recombinant protein vaccine. Here, we report a DNA vaccine containing the spike gene for delivery via electroporation. The spike genes of SARS-CoV and SARS-CoV-2 were codon optimized for mammalian cell expression and then cloned into mammalian cell expression vectors, called pSARS-S and pSARS2-S, respectively. Spike protein expression was confirmed by immunoblotting after transient expression in HEK293T cells. After immunization, sera were collected for antigen-specific antibody and neutralizing antibody titer analyses. We found that both pSARS-S and pSARS2-S immunization induced similar levels of antibodies against S2 of SARS-CoV-2. In contrast, only pSARS2-S immunization induced antibodies against the receptor-binding domain of SARS-CoV-2. We further found that pSARS2-S immunization, but not pSARS-S immunization, could induce very high titers of neutralizing antibodies against SARS-CoV-2. We further analyzed SARS-CoV-2 S protein-specific T cell responses and found that the immune responses were biased toward Th1. Importantly, pSARS2-S immunization in hamsters could induce protective immunity against SARS-CoV-2 challenge in vivo. These data suggest that DNA vaccination could be a promising approach for protecting against COVID-19.https://journals.plos.org/plosntds/article/file?id=10.1371/journal.pntd.0009374&type=printable |
| spellingShingle | Kit Man Chai Tsai-Teng Tzeng Kuan-Yin Shen Hung-Chun Liao Jhe-Jhih Lin Mei-Yu Chen Guann-Yi Yu Horng-Yunn Dou Ching-Len Liao Hsin-Wei Chen Shih-Jen Liu DNA vaccination induced protective immunity against SARS CoV-2 infection in hamsterss. PLoS Neglected Tropical Diseases |
| title | DNA vaccination induced protective immunity against SARS CoV-2 infection in hamsterss. |
| title_full | DNA vaccination induced protective immunity against SARS CoV-2 infection in hamsterss. |
| title_fullStr | DNA vaccination induced protective immunity against SARS CoV-2 infection in hamsterss. |
| title_full_unstemmed | DNA vaccination induced protective immunity against SARS CoV-2 infection in hamsterss. |
| title_short | DNA vaccination induced protective immunity against SARS CoV-2 infection in hamsterss. |
| title_sort | dna vaccination induced protective immunity against sars cov 2 infection in hamsterss |
| url | https://journals.plos.org/plosntds/article/file?id=10.1371/journal.pntd.0009374&type=printable |
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