Curcumin's protective role against fluoride-induced bone damage: Implications for pullet pathology and skeletal biomechanics

This study aimed to investigate the effects of dietary fluoride (F) and curcumin (Cur) supplementation on the tibial biomechanical performance, histopathology, and behavior of pullets. Four dietary F levels (0, 400, 800, 1200 mg/kg) supplemented with CUR (0, 200 mg/kg) were used to create 8 experime...

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Main Authors: Qi-yong Zuo, Hai-qiang Meng, Mohammad Mehdi Ommati, Guo-dong Yang, Wen-peng Zhao, Jing Zhao, Bian-hua Zhou, Hong-wei Wang
Format: Article
Language:English
Published: Elsevier 2025-03-01
Series:Poultry Science
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Online Access:http://www.sciencedirect.com/science/article/pii/S0032579125001282
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author Qi-yong Zuo
Hai-qiang Meng
Mohammad Mehdi Ommati
Guo-dong Yang
Wen-peng Zhao
Jing Zhao
Bian-hua Zhou
Hong-wei Wang
author_facet Qi-yong Zuo
Hai-qiang Meng
Mohammad Mehdi Ommati
Guo-dong Yang
Wen-peng Zhao
Jing Zhao
Bian-hua Zhou
Hong-wei Wang
author_sort Qi-yong Zuo
collection DOAJ
description This study aimed to investigate the effects of dietary fluoride (F) and curcumin (Cur) supplementation on the tibial biomechanical performance, histopathology, and behavior of pullets. Four dietary F levels (0, 400, 800, 1200 mg/kg) supplemented with CUR (0, 200 mg/kg) were used to create 8 experimental groups in Hyline Brown pullets. Behavioral study results showed that supplements of 1200 mg/kg F reduced the percentages of feeding, walking, standing, and preening behaviors while increasing the percentage of lying behavior (P < 0.05). This is associated with F-induced tibial elastic modulus, maximum stress, and stiffness coefficient were reduced and toughness coefficient was increased (P < 0.05). F-induced tibial cortical bone thickened, trabecular bone widened, and excessive accumulation of bone collagen fibers (P < 0.05) in the tibia explained the biomechanical properties reduction in Hyline Brown pullets. Additionally, the loss of antioxidant capacity was mediated by excessive F-accelerated pathological damage to the bone (P < 0.05). Supplementation with 200 mg/kg CUR alleviated abnormal behavior, expansion of the trabecular bone, accumulation of collagen fibers, and loss of antioxidant capacity (P < 0.05). In conclusion, F reduced the antioxidant level of the body, caused tibia histopathological damage, destroyed the tibia biomechanical properties, and caused abnormal behavior of pullets. Supplementation with 200 mg/kg CUR attenuates F-induced oxidative and tibia damage and rectifies abnormal behavioral traits.
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spelling doaj-art-ed8fbb3b898b4104862823a876ac2f0e2025-08-20T02:52:20ZengElsevierPoultry Science0032-57912025-03-01104310489110.1016/j.psj.2025.104891Curcumin's protective role against fluoride-induced bone damage: Implications for pullet pathology and skeletal biomechanicsQi-yong Zuo0Hai-qiang Meng1Mohammad Mehdi Ommati2Guo-dong Yang3Wen-peng Zhao4Jing Zhao5Bian-hua Zhou6Hong-wei Wang7Henan Key Laboratory of Environmental and Animal Product Safety, Henan University of Science and Technology, Kaiyuan Avenue 263, Luoyang, Henan 471000, PR ChinaHenan Key Laboratory of Environmental and Animal Product Safety, Henan University of Science and Technology, Kaiyuan Avenue 263, Luoyang, Henan 471000, PR ChinaHenan Key Laboratory of Environmental and Animal Product Safety, Henan University of Science and Technology, Kaiyuan Avenue 263, Luoyang, Henan 471000, PR ChinaHenan Key Laboratory of Environmental and Animal Product Safety, Henan University of Science and Technology, Kaiyuan Avenue 263, Luoyang, Henan 471000, PR ChinaHenan Key Laboratory of Environmental and Animal Product Safety, Henan University of Science and Technology, Kaiyuan Avenue 263, Luoyang, Henan 471000, PR ChinaHenan Key Laboratory of Environmental and Animal Product Safety, Henan University of Science and Technology, Kaiyuan Avenue 263, Luoyang, Henan 471000, PR ChinaHenan Key Laboratory of Environmental and Animal Product Safety, Henan University of Science and Technology, Kaiyuan Avenue 263, Luoyang, Henan 471000, PR ChinaCorresponding author.; Henan Key Laboratory of Environmental and Animal Product Safety, Henan University of Science and Technology, Kaiyuan Avenue 263, Luoyang, Henan 471000, PR ChinaThis study aimed to investigate the effects of dietary fluoride (F) and curcumin (Cur) supplementation on the tibial biomechanical performance, histopathology, and behavior of pullets. Four dietary F levels (0, 400, 800, 1200 mg/kg) supplemented with CUR (0, 200 mg/kg) were used to create 8 experimental groups in Hyline Brown pullets. Behavioral study results showed that supplements of 1200 mg/kg F reduced the percentages of feeding, walking, standing, and preening behaviors while increasing the percentage of lying behavior (P < 0.05). This is associated with F-induced tibial elastic modulus, maximum stress, and stiffness coefficient were reduced and toughness coefficient was increased (P < 0.05). F-induced tibial cortical bone thickened, trabecular bone widened, and excessive accumulation of bone collagen fibers (P < 0.05) in the tibia explained the biomechanical properties reduction in Hyline Brown pullets. Additionally, the loss of antioxidant capacity was mediated by excessive F-accelerated pathological damage to the bone (P < 0.05). Supplementation with 200 mg/kg CUR alleviated abnormal behavior, expansion of the trabecular bone, accumulation of collagen fibers, and loss of antioxidant capacity (P < 0.05). In conclusion, F reduced the antioxidant level of the body, caused tibia histopathological damage, destroyed the tibia biomechanical properties, and caused abnormal behavior of pullets. Supplementation with 200 mg/kg CUR attenuates F-induced oxidative and tibia damage and rectifies abnormal behavioral traits.http://www.sciencedirect.com/science/article/pii/S0032579125001282AntioxidantsHistopathologySkeletal biomechanicsBehaviorSkeletal fluorosis
spellingShingle Qi-yong Zuo
Hai-qiang Meng
Mohammad Mehdi Ommati
Guo-dong Yang
Wen-peng Zhao
Jing Zhao
Bian-hua Zhou
Hong-wei Wang
Curcumin's protective role against fluoride-induced bone damage: Implications for pullet pathology and skeletal biomechanics
Poultry Science
Antioxidants
Histopathology
Skeletal biomechanics
Behavior
Skeletal fluorosis
title Curcumin's protective role against fluoride-induced bone damage: Implications for pullet pathology and skeletal biomechanics
title_full Curcumin's protective role against fluoride-induced bone damage: Implications for pullet pathology and skeletal biomechanics
title_fullStr Curcumin's protective role against fluoride-induced bone damage: Implications for pullet pathology and skeletal biomechanics
title_full_unstemmed Curcumin's protective role against fluoride-induced bone damage: Implications for pullet pathology and skeletal biomechanics
title_short Curcumin's protective role against fluoride-induced bone damage: Implications for pullet pathology and skeletal biomechanics
title_sort curcumin s protective role against fluoride induced bone damage implications for pullet pathology and skeletal biomechanics
topic Antioxidants
Histopathology
Skeletal biomechanics
Behavior
Skeletal fluorosis
url http://www.sciencedirect.com/science/article/pii/S0032579125001282
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