Laser Capture Microdissection in Dentistry
Laser capture microdissection (LCM) allows for the microscopic procurement of specific cell types from tissue sections that can then be used for gene expression analysis. According to the recent development of the LCM technologies and methodologies, the LCM has been used in various kinds of tissue s...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2010-01-01
|
| Series: | International Journal of Dentistry |
| Online Access: | http://dx.doi.org/10.1155/2010/592694 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832556925520183296 |
|---|---|
| author | Uraiwan Chokechanachaisakul Tomoatsu Kaneko Takashi Okiji Reika Kaneko Hideaki Suda Jacques E. Nör |
| author_facet | Uraiwan Chokechanachaisakul Tomoatsu Kaneko Takashi Okiji Reika Kaneko Hideaki Suda Jacques E. Nör |
| author_sort | Uraiwan Chokechanachaisakul |
| collection | DOAJ |
| description | Laser capture microdissection (LCM) allows for the microscopic procurement of specific cell types from tissue sections that can then be used for gene expression analysis. According to the recent development of the LCM technologies and methodologies, the LCM has been used in various kinds of tissue specimens in dental research. For example, the real-time polymerase-chain reaction (PCR) can be performed from the formaldehyde-fixed, paraffin-embedded, and immunostained sections. Thus, the advance of immuno-LCM method allows us to improve the validity of molecular biological analysis and to get more accurate diagnosis in pathological field in contrast to conventional LCM. This paper is focused on the presentation and discussion of the existing literature that covers the fields of RNA analysis following LCM in dentistry. |
| format | Article |
| id | doaj-art-ed8ee5fe6c314ad0a9dcc62280e5e346 |
| institution | Kabale University |
| issn | 1687-8728 1687-8736 |
| language | English |
| publishDate | 2010-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Dentistry |
| spelling | doaj-art-ed8ee5fe6c314ad0a9dcc62280e5e3462025-02-03T05:44:10ZengWileyInternational Journal of Dentistry1687-87281687-87362010-01-01201010.1155/2010/592694592694Laser Capture Microdissection in DentistryUraiwan Chokechanachaisakul0Tomoatsu Kaneko1Takashi Okiji2Reika Kaneko3Hideaki Suda4Jacques E. Nör5Pulp Biology and Endodontics, Department of Restorative Sciences, Graduate School, Tokyo Medical and Dental University, 5-45, Yushima 1-chome, Bunkyo-ku, Tokyo 113-8549, JapanDivision of Cariology, Operative Dentistry and Endodontics, Department of Oral Health Science, Graduate School of Medical and Dental Sciences, Niigata University, 2-5274, Gakkocho-dori, Chuo-ku, Niigata 951-8514, JapanDivision of Cariology, Operative Dentistry and Endodontics, Department of Oral Health Science, Graduate School of Medical and Dental Sciences, Niigata University, 2-5274, Gakkocho-dori, Chuo-ku, Niigata 951-8514, JapanDepartment of Applied Molecular Medicine, Niigata University Graduate School of Medical and Dental Sciences, Niigata 951-8510, JapanPulp Biology and Endodontics, Department of Restorative Sciences, Graduate School, Tokyo Medical and Dental University, 5-45, Yushima 1-chome, Bunkyo-ku, Tokyo 113-8549, JapanDepartment of Cariology, Restorative Sciences, and Endodontics, Dental School, University of Michigan, 1011 N. University, Ann Arbor, MI 48109-1078, USALaser capture microdissection (LCM) allows for the microscopic procurement of specific cell types from tissue sections that can then be used for gene expression analysis. According to the recent development of the LCM technologies and methodologies, the LCM has been used in various kinds of tissue specimens in dental research. For example, the real-time polymerase-chain reaction (PCR) can be performed from the formaldehyde-fixed, paraffin-embedded, and immunostained sections. Thus, the advance of immuno-LCM method allows us to improve the validity of molecular biological analysis and to get more accurate diagnosis in pathological field in contrast to conventional LCM. This paper is focused on the presentation and discussion of the existing literature that covers the fields of RNA analysis following LCM in dentistry.http://dx.doi.org/10.1155/2010/592694 |
| spellingShingle | Uraiwan Chokechanachaisakul Tomoatsu Kaneko Takashi Okiji Reika Kaneko Hideaki Suda Jacques E. Nör Laser Capture Microdissection in Dentistry International Journal of Dentistry |
| title | Laser Capture Microdissection in Dentistry |
| title_full | Laser Capture Microdissection in Dentistry |
| title_fullStr | Laser Capture Microdissection in Dentistry |
| title_full_unstemmed | Laser Capture Microdissection in Dentistry |
| title_short | Laser Capture Microdissection in Dentistry |
| title_sort | laser capture microdissection in dentistry |
| url | http://dx.doi.org/10.1155/2010/592694 |
| work_keys_str_mv | AT uraiwanchokechanachaisakul lasercapturemicrodissectionindentistry AT tomoatsukaneko lasercapturemicrodissectionindentistry AT takashiokiji lasercapturemicrodissectionindentistry AT reikakaneko lasercapturemicrodissectionindentistry AT hideakisuda lasercapturemicrodissectionindentistry AT jacquesenor lasercapturemicrodissectionindentistry |