Biochar and Bacillus subtilis co-drive dryland soil microbial community and enzyme responses
IntroductionTo investigate the impact of soil amendments on the structure of the soil microbial community.MethodsThis study focuses on dryland soil and employs indoor static cultivation as the experimental approach. It analyzes the impact and mechanism of adding rice straw biochar (S), rapeseed stra...
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Frontiers Media S.A.
2025-05-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1603488/full |
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| author | Tao Zheng Tao Zheng Xianhuai Huang Xianhuai Huang Xiaoyu Zhou Jizi Wu Muhammad Aqeel Kamran Xiongsheng Yu Jing Qian Jing Qian |
| author_facet | Tao Zheng Tao Zheng Xianhuai Huang Xianhuai Huang Xiaoyu Zhou Jizi Wu Muhammad Aqeel Kamran Xiongsheng Yu Jing Qian Jing Qian |
| author_sort | Tao Zheng |
| collection | DOAJ |
| description | IntroductionTo investigate the impact of soil amendments on the structure of the soil microbial community.MethodsThis study focuses on dryland soil and employs indoor static cultivation as the experimental approach. It analyzes the impact and mechanism of adding rice straw biochar (S), rapeseed straw biochar (Y), and Bacillus subtilis agent (J) separately and in combination on the soil microbial community structure.ResultsThe experimental results indicated that, compared to the blank control (CK), the Y treatment increased the relative abundance of Proteobacteria by approximately 3.03% and significantly reduced the abundance of Acidobacteria (from 70.56% to 82.81%). The application of biochar and microbial inoculants significantly increased the relative abundance of Ascomycota (2.85% to 33.53%) and Rozellomycota (0.58% to 27.73%). Furthermore, the addition of soil amendments enhanced the richness (3.02% to 7.07%) and diversity (3.22% to 3.77%) of soil bacteria, as well as the microbial nitrogen content (3.7 to 9.3 times). Meanwhile, except for the YJ treatment, the richness of the fungal community decreased, while the diversity index increased. The experimental results showed that the application of rapeseed straw biochar or the compound microbial inoculant alone significantly increased soil urease activity, reaching 40.34 µg of NH+4-N g−1 of soil h−1 and 40.29 µg of NH+4-N g−1 of soil h−1 at the end of the incubation period, respectively.DiscussionIn conclusion, rapeseed straw biochar not only enhances the soil microbial community but also significantly influences soil enzyme activity. This study offers a scientific foundation for utilizing biochar and Bacillus subtilis to improve dryland soil, providing valuable insights for sustainable soil management. |
| format | Article |
| id | doaj-art-ed8ec6f300c449f78caaebda546ba9f6 |
| institution | Kabale University |
| issn | 1664-302X |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj-art-ed8ec6f300c449f78caaebda546ba9f62025-08-20T03:48:27ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2025-05-011610.3389/fmicb.2025.16034881603488Biochar and Bacillus subtilis co-drive dryland soil microbial community and enzyme responsesTao Zheng0Tao Zheng1Xianhuai Huang2Xianhuai Huang3Xiaoyu Zhou4Jizi Wu5Muhammad Aqeel Kamran6Xiongsheng Yu7Jing Qian8Jing Qian9School of Environment and Energy Engineering, Anhui JianZhu University, Hefei, ChinaAnhui Provincial Key Laboratory of Environmental Pollution Control and Resource Reuse, Hefei, ChinaSchool of Environment and Energy Engineering, Anhui JianZhu University, Hefei, ChinaAnhui Provincial Key Laboratory of Environmental Pollution Control and Resource Reuse, Hefei, ChinaHu Zhou Shi Zhi Bao Jian Yi Yu Geng Fei Guan Li Zhan, Huzhou, ChinaCollege of Environmental and Resource Sciences, Zhejiang A&F University, Lin'an, ChinaXishuangbanna Tropical Botanical Garden Yunnan Province, Mengla, Yunnan, ChinaDepartment of Chemistry, Xinzhou Normal University, Xinzhou, Shanxi, ChinaSchool of Environment and Energy Engineering, Anhui JianZhu University, Hefei, ChinaAnhui Provincial Key Laboratory of Environmental Pollution Control and Resource Reuse, Hefei, ChinaIntroductionTo investigate the impact of soil amendments on the structure of the soil microbial community.MethodsThis study focuses on dryland soil and employs indoor static cultivation as the experimental approach. It analyzes the impact and mechanism of adding rice straw biochar (S), rapeseed straw biochar (Y), and Bacillus subtilis agent (J) separately and in combination on the soil microbial community structure.ResultsThe experimental results indicated that, compared to the blank control (CK), the Y treatment increased the relative abundance of Proteobacteria by approximately 3.03% and significantly reduced the abundance of Acidobacteria (from 70.56% to 82.81%). The application of biochar and microbial inoculants significantly increased the relative abundance of Ascomycota (2.85% to 33.53%) and Rozellomycota (0.58% to 27.73%). Furthermore, the addition of soil amendments enhanced the richness (3.02% to 7.07%) and diversity (3.22% to 3.77%) of soil bacteria, as well as the microbial nitrogen content (3.7 to 9.3 times). Meanwhile, except for the YJ treatment, the richness of the fungal community decreased, while the diversity index increased. The experimental results showed that the application of rapeseed straw biochar or the compound microbial inoculant alone significantly increased soil urease activity, reaching 40.34 µg of NH+4-N g−1 of soil h−1 and 40.29 µg of NH+4-N g−1 of soil h−1 at the end of the incubation period, respectively.DiscussionIn conclusion, rapeseed straw biochar not only enhances the soil microbial community but also significantly influences soil enzyme activity. This study offers a scientific foundation for utilizing biochar and Bacillus subtilis to improve dryland soil, providing valuable insights for sustainable soil management.https://www.frontiersin.org/articles/10.3389/fmicb.2025.1603488/fullbiocharBacillus subtilisdryland soilbacterial communityfungal communities |
| spellingShingle | Tao Zheng Tao Zheng Xianhuai Huang Xianhuai Huang Xiaoyu Zhou Jizi Wu Muhammad Aqeel Kamran Xiongsheng Yu Jing Qian Jing Qian Biochar and Bacillus subtilis co-drive dryland soil microbial community and enzyme responses Frontiers in Microbiology biochar Bacillus subtilis dryland soil bacterial community fungal communities |
| title | Biochar and Bacillus subtilis co-drive dryland soil microbial community and enzyme responses |
| title_full | Biochar and Bacillus subtilis co-drive dryland soil microbial community and enzyme responses |
| title_fullStr | Biochar and Bacillus subtilis co-drive dryland soil microbial community and enzyme responses |
| title_full_unstemmed | Biochar and Bacillus subtilis co-drive dryland soil microbial community and enzyme responses |
| title_short | Biochar and Bacillus subtilis co-drive dryland soil microbial community and enzyme responses |
| title_sort | biochar and bacillus subtilis co drive dryland soil microbial community and enzyme responses |
| topic | biochar Bacillus subtilis dryland soil bacterial community fungal communities |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1603488/full |
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