Exercise Attenuates Skeletal Muscle Atrophy in Senescent SAMP8 Mice: Metabolic Insights from NMR-Based Metabolomics

Age-related skeletal muscle atrophy is a major health concern in the elderly, contributing to reduced mobility, increased risk of falls, and metabolic dysfunction. The senescence-accelerated prone 8 (SAMP8) mouse model, known for its rapid aging and early cognitive decline, serves as an essential mo...

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Main Authors: Wenfang Wu, Linglin Zhang, Yifen Chen, Caihua Huang, Longhe Yang, Donghai Lin
Format: Article
Language:English
Published: MDPI AG 2025-04-01
Series:Molecules
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Online Access:https://www.mdpi.com/1420-3049/30/9/2003
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author Wenfang Wu
Linglin Zhang
Yifen Chen
Caihua Huang
Longhe Yang
Donghai Lin
author_facet Wenfang Wu
Linglin Zhang
Yifen Chen
Caihua Huang
Longhe Yang
Donghai Lin
author_sort Wenfang Wu
collection DOAJ
description Age-related skeletal muscle atrophy is a major health concern in the elderly, contributing to reduced mobility, increased risk of falls, and metabolic dysfunction. The senescence-accelerated prone 8 (SAMP8) mouse model, known for its rapid aging and early cognitive decline, serves as an essential model for studying age-related muscle degeneration. While previous studies have shown that exercise attenuates muscle atrophy by promoting regeneration and improving strength, the underlying metabolic mechanisms remain poorly understood. This study used the SAMP8 model to evaluate the effects of exercise on muscle atrophy and associated metabolic changes. Our results show that exercise promoted muscle growth by reducing body weight, increasing skeletal muscle mass, and decreasing fat accumulation. Furthermore, exercise improved grip strength, muscle tone, and muscle fiber cross-sectional area, thereby preserving muscle functionality. NMR-based metabolomic analysis identified key metabolic pathways modulated by exercise, including glycine, serine, and threonine metabolism; alanine, aspartate, and glutamate metabolism; pyruvate metabolism; and taurine and hypotaurine metabolism. These findings underscore the therapeutic potential of exercise in combating age-related muscle wasting and elucidate the metabolic pathways underlying its benefits.
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spelling doaj-art-ed825959d7d445ffb0ff71ffdbfa50332025-08-20T02:59:11ZengMDPI AGMolecules1420-30492025-04-01309200310.3390/molecules30092003Exercise Attenuates Skeletal Muscle Atrophy in Senescent SAMP8 Mice: Metabolic Insights from NMR-Based MetabolomicsWenfang Wu0Linglin Zhang1Yifen Chen2Caihua Huang3Longhe Yang4Donghai Lin5Key Laboratory for Chemical Biology of Fujian Province, High-Field NMR Center, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, ChinaKey Laboratory for Chemical Biology of Fujian Province, High-Field NMR Center, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, ChinaKey Laboratory for Chemical Biology of Fujian Province, High-Field NMR Center, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, ChinaResearch and Communication Center of Exercise and Health, Xiamen University of Technology, Xiamen 361021, ChinaTechnical Innovation Center for Utilization of Marine Biological Resources, Third Institute of Oceanography, Ministry of Natural Resources, Xiamen 361021, ChinaKey Laboratory for Chemical Biology of Fujian Province, High-Field NMR Center, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, ChinaAge-related skeletal muscle atrophy is a major health concern in the elderly, contributing to reduced mobility, increased risk of falls, and metabolic dysfunction. The senescence-accelerated prone 8 (SAMP8) mouse model, known for its rapid aging and early cognitive decline, serves as an essential model for studying age-related muscle degeneration. While previous studies have shown that exercise attenuates muscle atrophy by promoting regeneration and improving strength, the underlying metabolic mechanisms remain poorly understood. This study used the SAMP8 model to evaluate the effects of exercise on muscle atrophy and associated metabolic changes. Our results show that exercise promoted muscle growth by reducing body weight, increasing skeletal muscle mass, and decreasing fat accumulation. Furthermore, exercise improved grip strength, muscle tone, and muscle fiber cross-sectional area, thereby preserving muscle functionality. NMR-based metabolomic analysis identified key metabolic pathways modulated by exercise, including glycine, serine, and threonine metabolism; alanine, aspartate, and glutamate metabolism; pyruvate metabolism; and taurine and hypotaurine metabolism. These findings underscore the therapeutic potential of exercise in combating age-related muscle wasting and elucidate the metabolic pathways underlying its benefits.https://www.mdpi.com/1420-3049/30/9/2003exerciseagingskeletal muscleNMR-based metabolomicsSAMP8
spellingShingle Wenfang Wu
Linglin Zhang
Yifen Chen
Caihua Huang
Longhe Yang
Donghai Lin
Exercise Attenuates Skeletal Muscle Atrophy in Senescent SAMP8 Mice: Metabolic Insights from NMR-Based Metabolomics
Molecules
exercise
aging
skeletal muscle
NMR-based metabolomics
SAMP8
title Exercise Attenuates Skeletal Muscle Atrophy in Senescent SAMP8 Mice: Metabolic Insights from NMR-Based Metabolomics
title_full Exercise Attenuates Skeletal Muscle Atrophy in Senescent SAMP8 Mice: Metabolic Insights from NMR-Based Metabolomics
title_fullStr Exercise Attenuates Skeletal Muscle Atrophy in Senescent SAMP8 Mice: Metabolic Insights from NMR-Based Metabolomics
title_full_unstemmed Exercise Attenuates Skeletal Muscle Atrophy in Senescent SAMP8 Mice: Metabolic Insights from NMR-Based Metabolomics
title_short Exercise Attenuates Skeletal Muscle Atrophy in Senescent SAMP8 Mice: Metabolic Insights from NMR-Based Metabolomics
title_sort exercise attenuates skeletal muscle atrophy in senescent samp8 mice metabolic insights from nmr based metabolomics
topic exercise
aging
skeletal muscle
NMR-based metabolomics
SAMP8
url https://www.mdpi.com/1420-3049/30/9/2003
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