Thermosonication-assisted fortification of kiwi juice with bee bread: enhancing nutritional and functional properties through ANFIS-RSM optimization

This study investigated the effects of thermosonication on the preservation and enhancement of bioactive components in kiwi juice fortified with bee bread. Response Surface Methodology (RSM) and Adaptive Neuro-Fuzzy Inference System (ANFIS) were employed to optimize processing parameters by evaluati...

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Main Authors: Seydi Yıkmış, Aylin Duman Altan, Melikenur Türkol, Nazlı Tokatlı, Çiğdem Yıldırım Maviş, Nazan Tokatlı Demirok, Rana Muhammad Aadil, Emad Karrar, Moneera O. Aljobair, Isam A. Mohamed Ahmed
Format: Article
Language:English
Published: Frontiers Media S.A. 2025-08-01
Series:Frontiers in Nutrition
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Online Access:https://www.frontiersin.org/articles/10.3389/fnut.2025.1657136/full
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author Seydi Yıkmış
Aylin Duman Altan
Melikenur Türkol
Nazlı Tokatlı
Çiğdem Yıldırım Maviş
Nazan Tokatlı Demirok
Rana Muhammad Aadil
Emad Karrar
Moneera O. Aljobair
Isam A. Mohamed Ahmed
author_facet Seydi Yıkmış
Aylin Duman Altan
Melikenur Türkol
Nazlı Tokatlı
Çiğdem Yıldırım Maviş
Nazan Tokatlı Demirok
Rana Muhammad Aadil
Emad Karrar
Moneera O. Aljobair
Isam A. Mohamed Ahmed
author_sort Seydi Yıkmış
collection DOAJ
description This study investigated the effects of thermosonication on the preservation and enhancement of bioactive components in kiwi juice fortified with bee bread. Response Surface Methodology (RSM) and Adaptive Neuro-Fuzzy Inference System (ANFIS) were employed to optimize processing parameters by evaluating FRAP, total phenolics, total chlorophyll, and ascorbic acid levels. Thermosonication significantly enhanced the levels of phenolic compounds (127.97 GAE mg/100 mL) and ascorbic acid (14.89 mg/100 mL), while a reduction in chlorophyll content was observed. The ANFIS model provided more accurate predictions compared to RSM, particularly under optimal processing conditions. Additionally, the thermosonication-treated kiwi juice with bee bread (TS-KJB) exhibited the highest antioxidant capacity, total flavonoid, and dietary fiber content. The findings demonstrate that thermosonication is an effective and sustainable technique for improving the functional and nutritional properties of bee bread-fortified kiwi juice. This approach offers a promising alternative for the production of additive- and preservative-free functional fruit juices.
format Article
id doaj-art-ed824e8553154ae1830a015f5bf99378
institution Kabale University
issn 2296-861X
language English
publishDate 2025-08-01
publisher Frontiers Media S.A.
record_format Article
series Frontiers in Nutrition
spelling doaj-art-ed824e8553154ae1830a015f5bf993782025-08-20T03:42:40ZengFrontiers Media S.A.Frontiers in Nutrition2296-861X2025-08-011210.3389/fnut.2025.16571361657136Thermosonication-assisted fortification of kiwi juice with bee bread: enhancing nutritional and functional properties through ANFIS-RSM optimizationSeydi Yıkmış0Aylin Duman Altan1Melikenur Türkol2Nazlı Tokatlı3Çiğdem Yıldırım Maviş4Nazan Tokatlı Demirok5Rana Muhammad Aadil6Emad Karrar7Moneera O. Aljobair8Isam A. Mohamed Ahmed9Department of Food Technology, Tekirdag Namık Kemal University, Tekirdag, TürkiyeDepartment of Industrial Engineering, Tekirdag Namık Kemal University, Tekirdağ, TürkiyeNutrition and Dietetics, Faculty of Health Sciences, Halic University, Istanbul, TürkiyeDepartment of Computer Engineering, Faculty of Engineering and Natural Sciences, Istanbul Health and Technology University, Istanbul, TürkiyeNutrition and Dietetics, Faculty of Health Sciences, Halic University, Istanbul, TürkiyeDepartment of Nutrition and Dietetics, Faculty of Health Sciences, Tekirdag Namık Kemal University, Tekirdag, TürkiyeNational Institute of Food Science and Technology, University of Agriculture, Faisalabad, PakistanDepartment of Plant Sciences, North Dakota State University, Fargo, ND, United StatesDepartment of Sports Health, College of Sports Sciences and Physical Activity, Princess Nourah Bint Abdulrahman University, Riyadh, Saudi ArabiaDepartment of Food Science and Nutrition, College of Food and Agricultural Sciences, King Saud University, Riyadh, Saudi ArabiaThis study investigated the effects of thermosonication on the preservation and enhancement of bioactive components in kiwi juice fortified with bee bread. Response Surface Methodology (RSM) and Adaptive Neuro-Fuzzy Inference System (ANFIS) were employed to optimize processing parameters by evaluating FRAP, total phenolics, total chlorophyll, and ascorbic acid levels. Thermosonication significantly enhanced the levels of phenolic compounds (127.97 GAE mg/100 mL) and ascorbic acid (14.89 mg/100 mL), while a reduction in chlorophyll content was observed. The ANFIS model provided more accurate predictions compared to RSM, particularly under optimal processing conditions. Additionally, the thermosonication-treated kiwi juice with bee bread (TS-KJB) exhibited the highest antioxidant capacity, total flavonoid, and dietary fiber content. The findings demonstrate that thermosonication is an effective and sustainable technique for improving the functional and nutritional properties of bee bread-fortified kiwi juice. This approach offers a promising alternative for the production of additive- and preservative-free functional fruit juices.https://www.frontiersin.org/articles/10.3389/fnut.2025.1657136/fulladaptive neuro-fuzzy inferencebee breadbioactive substanceskiwi juiceresponse surface methodologythermosonication
spellingShingle Seydi Yıkmış
Aylin Duman Altan
Melikenur Türkol
Nazlı Tokatlı
Çiğdem Yıldırım Maviş
Nazan Tokatlı Demirok
Rana Muhammad Aadil
Emad Karrar
Moneera O. Aljobair
Isam A. Mohamed Ahmed
Thermosonication-assisted fortification of kiwi juice with bee bread: enhancing nutritional and functional properties through ANFIS-RSM optimization
Frontiers in Nutrition
adaptive neuro-fuzzy inference
bee bread
bioactive substances
kiwi juice
response surface methodology
thermosonication
title Thermosonication-assisted fortification of kiwi juice with bee bread: enhancing nutritional and functional properties through ANFIS-RSM optimization
title_full Thermosonication-assisted fortification of kiwi juice with bee bread: enhancing nutritional and functional properties through ANFIS-RSM optimization
title_fullStr Thermosonication-assisted fortification of kiwi juice with bee bread: enhancing nutritional and functional properties through ANFIS-RSM optimization
title_full_unstemmed Thermosonication-assisted fortification of kiwi juice with bee bread: enhancing nutritional and functional properties through ANFIS-RSM optimization
title_short Thermosonication-assisted fortification of kiwi juice with bee bread: enhancing nutritional and functional properties through ANFIS-RSM optimization
title_sort thermosonication assisted fortification of kiwi juice with bee bread enhancing nutritional and functional properties through anfis rsm optimization
topic adaptive neuro-fuzzy inference
bee bread
bioactive substances
kiwi juice
response surface methodology
thermosonication
url https://www.frontiersin.org/articles/10.3389/fnut.2025.1657136/full
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