RNAi of Neuropeptide CCHamide-1 and Its Receptor Indicates Role in Feeding Behavior in the Pea Aphid, <i>Acyrthosiphon pisum</i>
Neuropeptide <i>CCHamide-1</i> (abbreviated as <i>CCHa1</i>) is a recently discovered peptide that is present in many arthropods and is the ligand of the <i>CCHa1R</i>, a member of the G protein-coupled receptors (GPCRs) superfamily, which plays a regulatory role...
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2024-11-01
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| author | Sohaib Shahid Muhammad Bilal Amir Tian-Bo Ding Tong-Xian Liu Guy Smagghe Yan Shi |
| author_facet | Sohaib Shahid Muhammad Bilal Amir Tian-Bo Ding Tong-Xian Liu Guy Smagghe Yan Shi |
| author_sort | Sohaib Shahid |
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| description | Neuropeptide <i>CCHamide-1</i> (abbreviated as <i>CCHa1</i>) is a recently discovered peptide that is present in many arthropods and is the ligand of the <i>CCHa1R</i>, a member of the G protein-coupled receptors (GPCRs) superfamily, which plays a regulatory role in diverse physiological processes such as feeding, circadian rhythm, insulin production, lipid metabolism, growth, and reproduction. However, the function of this gene in aphids is still unknown. Here, we characterized and determined the potential role of <i>CCHa1</i>/<i>CCHa1R</i> signaling in the pea aphid, <i>Acyrthosiphon pisum</i>, which is a notorious pest in agriculture. The docking analysis revealed that the <i>CCHa1</i> peptide binds to its receptor <i>CCHa1R</i> through specific amino acid residues, which are critical for maintaining the structural and functional integrity of the peptide–receptor complex. Quantitative real-time reverse transcription-PCR (qRT-PCR) revealed the expression levels of <i>CCHa1</i>/<i>CCHa1R</i> transcripts in different development stages and different tissues, indicating that the <i>CCHa1</i> expression was high in the first nymphal instar compared to the upcoming nymphal instars and adults, and was predominantly high in the brain. The <i>CCHa1</i>/<i>CCHa1R</i> transcript levels were significantly upregulated in starved aphids compared to fed aphids. Moreover, RNAi knockdown by the injection of dsRNA-<i>CCHa1</i> and dsRNA-<i>CCHa1R</i> significantly reduced the corresponding expression of the target gene and reduced their food intake in adult aphids, as revealed by the electrical penetration graph results. <i>CCHa1</i>/<i>CCHa1R</i>-silencing also reduced the reproduction, but not the survival, in <i>A. pisum</i>. Our data demonstrated that <i>CCHa1/CCHa1R</i> play a role in the regulation of feeding in <i>A. pisum</i>, suggesting a role of the <i>CCHa1</i> signaling pathway in the aphids relating to their nutritional status. |
| format | Article |
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| institution | DOAJ |
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| publishDate | 2024-11-01 |
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| spelling | doaj-art-ed7fcb0c710d4b48ad43ecdb278a1c422025-08-20T02:53:30ZengMDPI AGInsects2075-44502024-11-01151293910.3390/insects15120939RNAi of Neuropeptide CCHamide-1 and Its Receptor Indicates Role in Feeding Behavior in the Pea Aphid, <i>Acyrthosiphon pisum</i>Sohaib Shahid0Muhammad Bilal Amir1Tian-Bo Ding2Tong-Xian Liu3Guy Smagghe4Yan Shi5Shandong Engineering Research Center for Environment-Friendly Agricultural Pest Management, College of Plant Health and Medicine, Qingdao Agricultural University, Qingdao 266109, ChinaShandong Engineering Research Center for Environment-Friendly Agricultural Pest Management, College of Plant Health and Medicine, Qingdao Agricultural University, Qingdao 266109, ChinaShandong Engineering Research Center for Environment-Friendly Agricultural Pest Management, College of Plant Health and Medicine, Qingdao Agricultural University, Qingdao 266109, ChinaShandong Engineering Research Center for Environment-Friendly Agricultural Pest Management, College of Plant Health and Medicine, Qingdao Agricultural University, Qingdao 266109, ChinaInstitute of Entomology, Guizhou University, Guiyang 550025, ChinaShandong Engineering Research Center for Environment-Friendly Agricultural Pest Management, College of Plant Health and Medicine, Qingdao Agricultural University, Qingdao 266109, ChinaNeuropeptide <i>CCHamide-1</i> (abbreviated as <i>CCHa1</i>) is a recently discovered peptide that is present in many arthropods and is the ligand of the <i>CCHa1R</i>, a member of the G protein-coupled receptors (GPCRs) superfamily, which plays a regulatory role in diverse physiological processes such as feeding, circadian rhythm, insulin production, lipid metabolism, growth, and reproduction. However, the function of this gene in aphids is still unknown. Here, we characterized and determined the potential role of <i>CCHa1</i>/<i>CCHa1R</i> signaling in the pea aphid, <i>Acyrthosiphon pisum</i>, which is a notorious pest in agriculture. The docking analysis revealed that the <i>CCHa1</i> peptide binds to its receptor <i>CCHa1R</i> through specific amino acid residues, which are critical for maintaining the structural and functional integrity of the peptide–receptor complex. Quantitative real-time reverse transcription-PCR (qRT-PCR) revealed the expression levels of <i>CCHa1</i>/<i>CCHa1R</i> transcripts in different development stages and different tissues, indicating that the <i>CCHa1</i> expression was high in the first nymphal instar compared to the upcoming nymphal instars and adults, and was predominantly high in the brain. The <i>CCHa1</i>/<i>CCHa1R</i> transcript levels were significantly upregulated in starved aphids compared to fed aphids. Moreover, RNAi knockdown by the injection of dsRNA-<i>CCHa1</i> and dsRNA-<i>CCHa1R</i> significantly reduced the corresponding expression of the target gene and reduced their food intake in adult aphids, as revealed by the electrical penetration graph results. <i>CCHa1</i>/<i>CCHa1R</i>-silencing also reduced the reproduction, but not the survival, in <i>A. pisum</i>. Our data demonstrated that <i>CCHa1/CCHa1R</i> play a role in the regulation of feeding in <i>A. pisum</i>, suggesting a role of the <i>CCHa1</i> signaling pathway in the aphids relating to their nutritional status.https://www.mdpi.com/2075-4450/15/12/939<i>Acyrthosiphon pisum</i>neuropeptide<i>CCHamide-1</i>feeding behaviorparthenogenetic reproduction |
| spellingShingle | Sohaib Shahid Muhammad Bilal Amir Tian-Bo Ding Tong-Xian Liu Guy Smagghe Yan Shi RNAi of Neuropeptide CCHamide-1 and Its Receptor Indicates Role in Feeding Behavior in the Pea Aphid, <i>Acyrthosiphon pisum</i> Insects <i>Acyrthosiphon pisum</i> neuropeptide <i>CCHamide-1</i> feeding behavior parthenogenetic reproduction |
| title | RNAi of Neuropeptide CCHamide-1 and Its Receptor Indicates Role in Feeding Behavior in the Pea Aphid, <i>Acyrthosiphon pisum</i> |
| title_full | RNAi of Neuropeptide CCHamide-1 and Its Receptor Indicates Role in Feeding Behavior in the Pea Aphid, <i>Acyrthosiphon pisum</i> |
| title_fullStr | RNAi of Neuropeptide CCHamide-1 and Its Receptor Indicates Role in Feeding Behavior in the Pea Aphid, <i>Acyrthosiphon pisum</i> |
| title_full_unstemmed | RNAi of Neuropeptide CCHamide-1 and Its Receptor Indicates Role in Feeding Behavior in the Pea Aphid, <i>Acyrthosiphon pisum</i> |
| title_short | RNAi of Neuropeptide CCHamide-1 and Its Receptor Indicates Role in Feeding Behavior in the Pea Aphid, <i>Acyrthosiphon pisum</i> |
| title_sort | rnai of neuropeptide cchamide 1 and its receptor indicates role in feeding behavior in the pea aphid i acyrthosiphon pisum i |
| topic | <i>Acyrthosiphon pisum</i> neuropeptide <i>CCHamide-1</i> feeding behavior parthenogenetic reproduction |
| url | https://www.mdpi.com/2075-4450/15/12/939 |
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