Controllable intein splicing and N-terminal cleavage at mesophilic temperatures

Controllable intein splicing and N-terminal cleavage at mesophilic temperatures

Inteins (intervening proteins) interrupt host proteins and are removed through a protein splicing reaction that ligates adjacent N- and C-exteins. The ability of inteins to specifically rearrange peptide bonds has proven exceptionally useful in protein engineering, thus, methods to control intein ac...

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Bibliographic Details
Main Authors: Taylor A. McNeal, Joel Weinberger, Geraldy L. S. Liman, Tia M. Ariagno, David W. Wood, Thomas J. Santangelo, Christopher W. Lennon
Format: Article
Language:English
Published: Frontiers Media S.A. 2025-02-01
Series:Frontiers in Bioengineering and Biotechnology
Subjects:
intein
protein splicing
N-terminal cleavage
bioseparations
biosensor
Online Access:https://www.frontiersin.org/articles/10.3389/fbioe.2025.1543573/full
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