Study of hemostasis gene polymorphisms in patients with gout

Abstract Background Gout is a chronic metabolic disorder frequently complicated by thrombotic events. Genetic variants in hemostasis-related genes may contribute to individual susceptibility to thrombosis in gout patients. Objective To assess the association between polymorphisms in hemostasis genes...

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Main Authors: Akramova Nigora Turdiqulovna, Nabiyeva Dildora Abdumalikovna, Bobomurodov Turdikul Akramovich, Bobomurodova Dilnoza Turdikulovna
Format: Article
Language:English
Published: SpringerOpen 2025-07-01
Series:Egyptian Journal of Medical Human Genetics
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Online Access:https://doi.org/10.1186/s43042-025-00753-0
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author Akramova Nigora Turdiqulovna
Nabiyeva Dildora Abdumalikovna
Bobomurodov Turdikul Akramovich
Bobomurodova Dilnoza Turdikulovna
author_facet Akramova Nigora Turdiqulovna
Nabiyeva Dildora Abdumalikovna
Bobomurodov Turdikul Akramovich
Bobomurodova Dilnoza Turdikulovna
author_sort Akramova Nigora Turdiqulovna
collection DOAJ
description Abstract Background Gout is a chronic metabolic disorder frequently complicated by thrombotic events. Genetic variants in hemostasis-related genes may contribute to individual susceptibility to thrombosis in gout patients. Objective To assess the association between polymorphisms in hemostasis genes and thrombotic risk in patients with gout. Methods A case–control study was conducted involving 83 patients with gout and 41 healthy controls. Genomic DNA was extracted from peripheral blood leukocytes. Polymorphisms in MTHFR (C677T), F2 (G20210A), F5 (G1691A), and PAI-1 (675 4G/5G) genes were analyzed using multiplex TaqMan real-time PCR. Results Significant differences were observed for MTHFR C677T and PAI-1 4G/5G polymorphisms. The MTHFR CC and PAI-1 4G/4G genotypes were more frequent in the gout group, potentially indicating increased thrombotic risk. In contrast, the MTHFR CT and PAI-1 5G/5G genotypes were more common in controls. No statistically significant associations were found for F2 and F5 variants. Conclusion Our findings suggest that MTHFR C677T and PAI-1 4G/5G polymorphisms may contribute to thrombotic predisposition in patients with gout. These results underscore the need for further research to clarify the clinical relevance of these variants in thrombosis risk stratification.
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spelling doaj-art-ed73725e7a204ec6a164cfc4d9d740df2025-08-20T04:03:01ZengSpringerOpenEgyptian Journal of Medical Human Genetics2090-24412025-07-012611510.1186/s43042-025-00753-0Study of hemostasis gene polymorphisms in patients with goutAkramova Nigora Turdiqulovna0Nabiyeva Dildora Abdumalikovna1Bobomurodov Turdikul Akramovich2Bobomurodova Dilnoza Turdikulovna3Department of Faculty and Hospital Therapy, Occupational Diseases No.1, Tashkent Medical AcademyDepartment of Faculty and Hospital Therapy, Occupational Diseases No.1, Tashkent Medical AcademyDepartment of Propaedeutics of Children’s Diseases No. 2, Tashkent Medical AcademyDepartment of Stomatology, Tashkent Medical AcademyAbstract Background Gout is a chronic metabolic disorder frequently complicated by thrombotic events. Genetic variants in hemostasis-related genes may contribute to individual susceptibility to thrombosis in gout patients. Objective To assess the association between polymorphisms in hemostasis genes and thrombotic risk in patients with gout. Methods A case–control study was conducted involving 83 patients with gout and 41 healthy controls. Genomic DNA was extracted from peripheral blood leukocytes. Polymorphisms in MTHFR (C677T), F2 (G20210A), F5 (G1691A), and PAI-1 (675 4G/5G) genes were analyzed using multiplex TaqMan real-time PCR. Results Significant differences were observed for MTHFR C677T and PAI-1 4G/5G polymorphisms. The MTHFR CC and PAI-1 4G/4G genotypes were more frequent in the gout group, potentially indicating increased thrombotic risk. In contrast, the MTHFR CT and PAI-1 5G/5G genotypes were more common in controls. No statistically significant associations were found for F2 and F5 variants. Conclusion Our findings suggest that MTHFR C677T and PAI-1 4G/5G polymorphisms may contribute to thrombotic predisposition in patients with gout. These results underscore the need for further research to clarify the clinical relevance of these variants in thrombosis risk stratification.https://doi.org/10.1186/s43042-025-00753-0GoutThrombosisGene polymorphismMTHFRPAI-1Hemostasis
spellingShingle Akramova Nigora Turdiqulovna
Nabiyeva Dildora Abdumalikovna
Bobomurodov Turdikul Akramovich
Bobomurodova Dilnoza Turdikulovna
Study of hemostasis gene polymorphisms in patients with gout
Egyptian Journal of Medical Human Genetics
Gout
Thrombosis
Gene polymorphism
MTHFR
PAI-1
Hemostasis
title Study of hemostasis gene polymorphisms in patients with gout
title_full Study of hemostasis gene polymorphisms in patients with gout
title_fullStr Study of hemostasis gene polymorphisms in patients with gout
title_full_unstemmed Study of hemostasis gene polymorphisms in patients with gout
title_short Study of hemostasis gene polymorphisms in patients with gout
title_sort study of hemostasis gene polymorphisms in patients with gout
topic Gout
Thrombosis
Gene polymorphism
MTHFR
PAI-1
Hemostasis
url https://doi.org/10.1186/s43042-025-00753-0
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