<i>Massilia paldalensis</i> sp. nov., Isolated from Stream Bank Soil
A novel rod-shaped, Gram-negative, motile, aerobic and heavy metal-resistant bacterial strain, designated TN1-12<sup>T</sup>, was isolated from stream bank soil in Paldal district, Suwon City, Republic of Korea. Growth occurred at 10–40 °C (opt 30 °C), NaCl concentrations up to 2% (<i...
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2025-05-01
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| author | Nhi Thi Ai Nguyen Jaisoo Kim |
| author_facet | Nhi Thi Ai Nguyen Jaisoo Kim |
| author_sort | Nhi Thi Ai Nguyen |
| collection | DOAJ |
| description | A novel rod-shaped, Gram-negative, motile, aerobic and heavy metal-resistant bacterial strain, designated TN1-12<sup>T</sup>, was isolated from stream bank soil in Paldal district, Suwon City, Republic of Korea. Growth occurred at 10–40 °C (opt 30 °C), NaCl concentrations up to 2% (<i>w</i>/<i>v</i>) and pH 5.0–8.0 (opt pH 7.0). Based on the 16S rRNA gene sequence, the closest relatives of strain, TN1-12<sup>T</sup>, are <i>Massilia putida</i> 6NM-7<sup>T</sup> (98.21% similarity), <i>Massilia forsythiae</i> GN2-R2<sup>T</sup> (98.00%), <i>Massilia rhizosphaerae</i> NEAU-GH312<sup>T</sup> (97.79%), <i>Massilia aurea</i> AP13<sup>T</sup> (97.78%) and <i>Massilia niabensis</i> 5420S-26<sup>T</sup> (97.71%). The predominant ubiquinone is Q-8. The G+C content of the DNA is 66.7 mol%. The major polar lipids are diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine. The major fatty acids are C16:0, summed feature 3 (C16:1ω7c and/or iso-C15:0 2-OH), 17:0 cyclo and summed feature 8 (C18:1 ω7c and/or C18:1 ω6c). DNA–DNA hybridization and Average Nucleotide Identity data showed values below 26% and 85%, respectively, confirming that TN1-12<sup>T</sup> represents a novel species. Based on the genotypic, chemotaxonomic and physiological data presented in this study, we propose that strain TN1-12<sup>T</sup> represents a novel species within the genus <i>Massilia</i> with the name <i>Massilia paldalensis</i> sp. nov. (=KACC 23946<sup>T</sup> = CGMCC 1.65296<sup>T</sup>). |
| format | Article |
| id | doaj-art-ed5633c226d64f429a625d303c8ef534 |
| institution | Kabale University |
| issn | 1424-2818 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Diversity |
| spelling | doaj-art-ed5633c226d64f429a625d303c8ef5342025-08-20T03:47:52ZengMDPI AGDiversity1424-28182025-05-0117532710.3390/d17050327<i>Massilia paldalensis</i> sp. nov., Isolated from Stream Bank SoilNhi Thi Ai Nguyen0Jaisoo Kim1Department of Life Science, College of Natural Sciences, Kyonggi University, Suwon 16227, Gyeonggi-do, Republic of KoreaDepartment of Life Science, College of Natural Sciences, Kyonggi University, Suwon 16227, Gyeonggi-do, Republic of KoreaA novel rod-shaped, Gram-negative, motile, aerobic and heavy metal-resistant bacterial strain, designated TN1-12<sup>T</sup>, was isolated from stream bank soil in Paldal district, Suwon City, Republic of Korea. Growth occurred at 10–40 °C (opt 30 °C), NaCl concentrations up to 2% (<i>w</i>/<i>v</i>) and pH 5.0–8.0 (opt pH 7.0). Based on the 16S rRNA gene sequence, the closest relatives of strain, TN1-12<sup>T</sup>, are <i>Massilia putida</i> 6NM-7<sup>T</sup> (98.21% similarity), <i>Massilia forsythiae</i> GN2-R2<sup>T</sup> (98.00%), <i>Massilia rhizosphaerae</i> NEAU-GH312<sup>T</sup> (97.79%), <i>Massilia aurea</i> AP13<sup>T</sup> (97.78%) and <i>Massilia niabensis</i> 5420S-26<sup>T</sup> (97.71%). The predominant ubiquinone is Q-8. The G+C content of the DNA is 66.7 mol%. The major polar lipids are diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine. The major fatty acids are C16:0, summed feature 3 (C16:1ω7c and/or iso-C15:0 2-OH), 17:0 cyclo and summed feature 8 (C18:1 ω7c and/or C18:1 ω6c). DNA–DNA hybridization and Average Nucleotide Identity data showed values below 26% and 85%, respectively, confirming that TN1-12<sup>T</sup> represents a novel species. Based on the genotypic, chemotaxonomic and physiological data presented in this study, we propose that strain TN1-12<sup>T</sup> represents a novel species within the genus <i>Massilia</i> with the name <i>Massilia paldalensis</i> sp. nov. (=KACC 23946<sup>T</sup> = CGMCC 1.65296<sup>T</sup>).https://www.mdpi.com/1424-2818/17/5/327<i>Massilia paldalensis</i>stream bank soilnew speciesheavy metal resistance |
| spellingShingle | Nhi Thi Ai Nguyen Jaisoo Kim <i>Massilia paldalensis</i> sp. nov., Isolated from Stream Bank Soil Diversity <i>Massilia paldalensis</i> stream bank soil new species heavy metal resistance |
| title | <i>Massilia paldalensis</i> sp. nov., Isolated from Stream Bank Soil |
| title_full | <i>Massilia paldalensis</i> sp. nov., Isolated from Stream Bank Soil |
| title_fullStr | <i>Massilia paldalensis</i> sp. nov., Isolated from Stream Bank Soil |
| title_full_unstemmed | <i>Massilia paldalensis</i> sp. nov., Isolated from Stream Bank Soil |
| title_short | <i>Massilia paldalensis</i> sp. nov., Isolated from Stream Bank Soil |
| title_sort | i massilia paldalensis i sp nov isolated from stream bank soil |
| topic | <i>Massilia paldalensis</i> stream bank soil new species heavy metal resistance |
| url | https://www.mdpi.com/1424-2818/17/5/327 |
| work_keys_str_mv | AT nhithiainguyen imassiliapaldalensisispnovisolatedfromstreambanksoil AT jaisookim imassiliapaldalensisispnovisolatedfromstreambanksoil |