<i>Massilia paldalensis</i> sp. nov., Isolated from Stream Bank Soil
A novel rod-shaped, Gram-negative, motile, aerobic and heavy metal-resistant bacterial strain, designated TN1-12<sup>T</sup>, was isolated from stream bank soil in Paldal district, Suwon City, Republic of Korea. Growth occurred at 10–40 °C (opt 30 °C), NaCl concentrations up to 2% (<i...
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| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-05-01
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| Series: | Diversity |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1424-2818/17/5/327 |
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| Summary: | A novel rod-shaped, Gram-negative, motile, aerobic and heavy metal-resistant bacterial strain, designated TN1-12<sup>T</sup>, was isolated from stream bank soil in Paldal district, Suwon City, Republic of Korea. Growth occurred at 10–40 °C (opt 30 °C), NaCl concentrations up to 2% (<i>w</i>/<i>v</i>) and pH 5.0–8.0 (opt pH 7.0). Based on the 16S rRNA gene sequence, the closest relatives of strain, TN1-12<sup>T</sup>, are <i>Massilia putida</i> 6NM-7<sup>T</sup> (98.21% similarity), <i>Massilia forsythiae</i> GN2-R2<sup>T</sup> (98.00%), <i>Massilia rhizosphaerae</i> NEAU-GH312<sup>T</sup> (97.79%), <i>Massilia aurea</i> AP13<sup>T</sup> (97.78%) and <i>Massilia niabensis</i> 5420S-26<sup>T</sup> (97.71%). The predominant ubiquinone is Q-8. The G+C content of the DNA is 66.7 mol%. The major polar lipids are diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine. The major fatty acids are C16:0, summed feature 3 (C16:1ω7c and/or iso-C15:0 2-OH), 17:0 cyclo and summed feature 8 (C18:1 ω7c and/or C18:1 ω6c). DNA–DNA hybridization and Average Nucleotide Identity data showed values below 26% and 85%, respectively, confirming that TN1-12<sup>T</sup> represents a novel species. Based on the genotypic, chemotaxonomic and physiological data presented in this study, we propose that strain TN1-12<sup>T</sup> represents a novel species within the genus <i>Massilia</i> with the name <i>Massilia paldalensis</i> sp. nov. (=KACC 23946<sup>T</sup> = CGMCC 1.65296<sup>T</sup>). |
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| ISSN: | 1424-2818 |