An Mpox Multi-Antigen-Tandem Bivalent mRNA Candidate Vaccine Effectively Protects Mice Against the Vaccinia Virus
Background: Since the outbreak of mpox in 2022, the disease has spread rapidly worldwide and garnered significant public attention. Vaccination is regarded as an effective measure to prevent the spread of mpox. The success of the COVID-19 mRNA vaccine demonstrates that mRNA-based vaccines represent...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-03-01
|
| Series: | Vaccines |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-393X/13/4/374 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849713497573687296 |
|---|---|
| author | Jun Zuo Jiayu Wu Zhen Zhang Jinrong Long Changxiao Yu Yuqin Liao Hongsheng Zhang Jing Yang |
| author_facet | Jun Zuo Jiayu Wu Zhen Zhang Jinrong Long Changxiao Yu Yuqin Liao Hongsheng Zhang Jing Yang |
| author_sort | Jun Zuo |
| collection | DOAJ |
| description | Background: Since the outbreak of mpox in 2022, the disease has spread rapidly worldwide and garnered significant public attention. Vaccination is regarded as an effective measure to prevent the spread of mpox. The success of the COVID-19 mRNA vaccine demonstrates that mRNA-based vaccines represent a rapid and multifunctional platform with considerable potential, and are expected to be a strategy to address mpox spread. Methods: In this study, we screened an mpox multi-antigen-tandem bivalent mRNA vaccine candidate: a lipid nanoparticle-encapsulated mRNA-1017 and mRNA-1995 (mRNA-3012-LNP). We then evaluated the immunogenicity of the mpox virus (MPXV) bivalent mRNA vaccine candidate and its protective efficacy against the vaccinia virus (VACV) in a mouse model. Results: Mice vaccinated with two doses of the mRNA-3012-LNP vaccine exhibited robust binding antibody responses and MPXV-specific Th-1-biased cellular immune responses in vivo. Notably, the boosted immunized mice generated potent neutralizing antibodies against the VACV, effectively protecting them from viral challenge. Additionally, serum transfer protection experiments indicated that serum from mice inoculated with mRNA-3012-LNP was effective in protecting nude mice from VACV challenge. Conclusions: Our results suggest that the mpox bivalent mRNA candidate vaccine mRNA-3012-LNP induces strong immunogenicity and has the potential to serve as a safe and effective vaccine candidate against mpox epidemics. |
| format | Article |
| id | doaj-art-ed51fb60882a40c68079e368164a691d |
| institution | DOAJ |
| issn | 2076-393X |
| language | English |
| publishDate | 2025-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Vaccines |
| spelling | doaj-art-ed51fb60882a40c68079e368164a691d2025-08-20T03:13:57ZengMDPI AGVaccines2076-393X2025-03-0113437410.3390/vaccines13040374An Mpox Multi-Antigen-Tandem Bivalent mRNA Candidate Vaccine Effectively Protects Mice Against the Vaccinia VirusJun Zuo0Jiayu Wu1Zhen Zhang2Jinrong Long3Changxiao Yu4Yuqin Liao5Hongsheng Zhang6Jing Yang7College of Chemistry and Life Science, Beijing University of Technology, Pingleyuan 100#, District of Chaoyang, Beijing 100124, ChinaBioinformatics Center of AMMS, Beijing 100850, ChinaBioinformatics Center of AMMS, Beijing 100850, ChinaBioinformatics Center of AMMS, Beijing 100850, ChinaBioinformatics Center of AMMS, Beijing 100850, ChinaBioinformatics Center of AMMS, Beijing 100850, ChinaCollege of Chemistry and Life Science, Beijing University of Technology, Pingleyuan 100#, District of Chaoyang, Beijing 100124, ChinaBioinformatics Center of AMMS, Beijing 100850, ChinaBackground: Since the outbreak of mpox in 2022, the disease has spread rapidly worldwide and garnered significant public attention. Vaccination is regarded as an effective measure to prevent the spread of mpox. The success of the COVID-19 mRNA vaccine demonstrates that mRNA-based vaccines represent a rapid and multifunctional platform with considerable potential, and are expected to be a strategy to address mpox spread. Methods: In this study, we screened an mpox multi-antigen-tandem bivalent mRNA vaccine candidate: a lipid nanoparticle-encapsulated mRNA-1017 and mRNA-1995 (mRNA-3012-LNP). We then evaluated the immunogenicity of the mpox virus (MPXV) bivalent mRNA vaccine candidate and its protective efficacy against the vaccinia virus (VACV) in a mouse model. Results: Mice vaccinated with two doses of the mRNA-3012-LNP vaccine exhibited robust binding antibody responses and MPXV-specific Th-1-biased cellular immune responses in vivo. Notably, the boosted immunized mice generated potent neutralizing antibodies against the VACV, effectively protecting them from viral challenge. Additionally, serum transfer protection experiments indicated that serum from mice inoculated with mRNA-3012-LNP was effective in protecting nude mice from VACV challenge. Conclusions: Our results suggest that the mpox bivalent mRNA candidate vaccine mRNA-3012-LNP induces strong immunogenicity and has the potential to serve as a safe and effective vaccine candidate against mpox epidemics.https://www.mdpi.com/2076-393X/13/4/374mpoxtandembivalentmRNA vaccineVACV |
| spellingShingle | Jun Zuo Jiayu Wu Zhen Zhang Jinrong Long Changxiao Yu Yuqin Liao Hongsheng Zhang Jing Yang An Mpox Multi-Antigen-Tandem Bivalent mRNA Candidate Vaccine Effectively Protects Mice Against the Vaccinia Virus Vaccines mpox tandem bivalent mRNA vaccine VACV |
| title | An Mpox Multi-Antigen-Tandem Bivalent mRNA Candidate Vaccine Effectively Protects Mice Against the Vaccinia Virus |
| title_full | An Mpox Multi-Antigen-Tandem Bivalent mRNA Candidate Vaccine Effectively Protects Mice Against the Vaccinia Virus |
| title_fullStr | An Mpox Multi-Antigen-Tandem Bivalent mRNA Candidate Vaccine Effectively Protects Mice Against the Vaccinia Virus |
| title_full_unstemmed | An Mpox Multi-Antigen-Tandem Bivalent mRNA Candidate Vaccine Effectively Protects Mice Against the Vaccinia Virus |
| title_short | An Mpox Multi-Antigen-Tandem Bivalent mRNA Candidate Vaccine Effectively Protects Mice Against the Vaccinia Virus |
| title_sort | mpox multi antigen tandem bivalent mrna candidate vaccine effectively protects mice against the vaccinia virus |
| topic | mpox tandem bivalent mRNA vaccine VACV |
| url | https://www.mdpi.com/2076-393X/13/4/374 |
| work_keys_str_mv | AT junzuo anmpoxmultiantigentandembivalentmrnacandidatevaccineeffectivelyprotectsmiceagainstthevacciniavirus AT jiayuwu anmpoxmultiantigentandembivalentmrnacandidatevaccineeffectivelyprotectsmiceagainstthevacciniavirus AT zhenzhang anmpoxmultiantigentandembivalentmrnacandidatevaccineeffectivelyprotectsmiceagainstthevacciniavirus AT jinronglong anmpoxmultiantigentandembivalentmrnacandidatevaccineeffectivelyprotectsmiceagainstthevacciniavirus AT changxiaoyu anmpoxmultiantigentandembivalentmrnacandidatevaccineeffectivelyprotectsmiceagainstthevacciniavirus AT yuqinliao anmpoxmultiantigentandembivalentmrnacandidatevaccineeffectivelyprotectsmiceagainstthevacciniavirus AT hongshengzhang anmpoxmultiantigentandembivalentmrnacandidatevaccineeffectivelyprotectsmiceagainstthevacciniavirus AT jingyang anmpoxmultiantigentandembivalentmrnacandidatevaccineeffectivelyprotectsmiceagainstthevacciniavirus AT junzuo mpoxmultiantigentandembivalentmrnacandidatevaccineeffectivelyprotectsmiceagainstthevacciniavirus AT jiayuwu mpoxmultiantigentandembivalentmrnacandidatevaccineeffectivelyprotectsmiceagainstthevacciniavirus AT zhenzhang mpoxmultiantigentandembivalentmrnacandidatevaccineeffectivelyprotectsmiceagainstthevacciniavirus AT jinronglong mpoxmultiantigentandembivalentmrnacandidatevaccineeffectivelyprotectsmiceagainstthevacciniavirus AT changxiaoyu mpoxmultiantigentandembivalentmrnacandidatevaccineeffectivelyprotectsmiceagainstthevacciniavirus AT yuqinliao mpoxmultiantigentandembivalentmrnacandidatevaccineeffectivelyprotectsmiceagainstthevacciniavirus AT hongshengzhang mpoxmultiantigentandembivalentmrnacandidatevaccineeffectivelyprotectsmiceagainstthevacciniavirus AT jingyang mpoxmultiantigentandembivalentmrnacandidatevaccineeffectivelyprotectsmiceagainstthevacciniavirus |