Alternative Expression Pattern of MALT1-A20-NF-κB in Patients with Rheumatoid Arthritis
Rheumatoid arthritis (RA) is an inflammatory autoimmune disorder; abnormal T cell immunity plays a critical role in the development of RA. Recently, A20 was identified as a key negative regulator for T cell activation and inflammatory signaling and may be involved in RA pathogenesis. In this study,...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2014/492872 |
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| author | Xu Wang Lihua Zhu Ziwei Liao Fan Zhang Ling Xu Yan Xu Shaohua Chen Lijian Yang Yi Zhou Yangqiu Li |
| author_facet | Xu Wang Lihua Zhu Ziwei Liao Fan Zhang Ling Xu Yan Xu Shaohua Chen Lijian Yang Yi Zhou Yangqiu Li |
| author_sort | Xu Wang |
| collection | DOAJ |
| description | Rheumatoid arthritis (RA) is an inflammatory autoimmune disorder; abnormal T cell immunity plays a critical role in the development of RA. Recently, A20 was identified as a key negative regulator for T cell activation and inflammatory signaling and may be involved in RA pathogenesis. In this study, we analyzed the expression level of A20, NF-κB, and A20 regulatory factor mucosa-associated lymphoid tissue lymphoma translocation gene 1 (MALT1) in patients with RA. Real-time PCR was used to determine the expression level of MALT1, MALT-V1, A20, and NF-κB genes in RA and healthy individuals (HI). Significantly lower A20 expression was found in RA patients compared with those in the healthy group, while NF-κB overexpression could be detected in patients with RA. Moreover, the MALT1 and MALT1-V1 expression level was downregulated in RA patients. A positive correlation between MALT1 and A20 and MALT1-V1 and A20 was found in patients with RA, and a tendency towards a negative correlation was found between MALT1 and NF-κB, MALT1-V1 and NF-κB, and A20 and NF-κB. In conclusion, we first characterized the alternative expression pattern of MALT1, A20, and NF-κB in RA, which may be related to abnormal T cell activation. |
| format | Article |
| id | doaj-art-ed347cf34feb45fb887194459c46b534 |
| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-ed347cf34feb45fb887194459c46b5342025-02-03T06:13:18ZengWileyJournal of Immunology Research2314-88612314-71562014-01-01201410.1155/2014/492872492872Alternative Expression Pattern of MALT1-A20-NF-κB in Patients with Rheumatoid ArthritisXu Wang0Lihua Zhu1Ziwei Liao2Fan Zhang3Ling Xu4Yan Xu5Shaohua Chen6Lijian Yang7Yi Zhou8Yangqiu Li9Institute of Hematology, Jinan University, Guangzhou 510632, ChinaInstitute of Hematology, Jinan University, Guangzhou 510632, ChinaInstitute of Hematology, Jinan University, Guangzhou 510632, ChinaInstitute of Hematology, Jinan University, Guangzhou 510632, ChinaInstitute of Hematology, Jinan University, Guangzhou 510632, ChinaInstitute of Hematology, Jinan University, Guangzhou 510632, ChinaInstitute of Hematology, Jinan University, Guangzhou 510632, ChinaInstitute of Hematology, Jinan University, Guangzhou 510632, ChinaDepartment of Rheumatism and Immunology, First Hospital Affiliated, Jinan University, Guangzhou 510632, ChinaInstitute of Hematology, Jinan University, Guangzhou 510632, ChinaRheumatoid arthritis (RA) is an inflammatory autoimmune disorder; abnormal T cell immunity plays a critical role in the development of RA. Recently, A20 was identified as a key negative regulator for T cell activation and inflammatory signaling and may be involved in RA pathogenesis. In this study, we analyzed the expression level of A20, NF-κB, and A20 regulatory factor mucosa-associated lymphoid tissue lymphoma translocation gene 1 (MALT1) in patients with RA. Real-time PCR was used to determine the expression level of MALT1, MALT-V1, A20, and NF-κB genes in RA and healthy individuals (HI). Significantly lower A20 expression was found in RA patients compared with those in the healthy group, while NF-κB overexpression could be detected in patients with RA. Moreover, the MALT1 and MALT1-V1 expression level was downregulated in RA patients. A positive correlation between MALT1 and A20 and MALT1-V1 and A20 was found in patients with RA, and a tendency towards a negative correlation was found between MALT1 and NF-κB, MALT1-V1 and NF-κB, and A20 and NF-κB. In conclusion, we first characterized the alternative expression pattern of MALT1, A20, and NF-κB in RA, which may be related to abnormal T cell activation.http://dx.doi.org/10.1155/2014/492872 |
| spellingShingle | Xu Wang Lihua Zhu Ziwei Liao Fan Zhang Ling Xu Yan Xu Shaohua Chen Lijian Yang Yi Zhou Yangqiu Li Alternative Expression Pattern of MALT1-A20-NF-κB in Patients with Rheumatoid Arthritis Journal of Immunology Research |
| title | Alternative Expression Pattern of MALT1-A20-NF-κB in Patients with Rheumatoid Arthritis |
| title_full | Alternative Expression Pattern of MALT1-A20-NF-κB in Patients with Rheumatoid Arthritis |
| title_fullStr | Alternative Expression Pattern of MALT1-A20-NF-κB in Patients with Rheumatoid Arthritis |
| title_full_unstemmed | Alternative Expression Pattern of MALT1-A20-NF-κB in Patients with Rheumatoid Arthritis |
| title_short | Alternative Expression Pattern of MALT1-A20-NF-κB in Patients with Rheumatoid Arthritis |
| title_sort | alternative expression pattern of malt1 a20 nf κb in patients with rheumatoid arthritis |
| url | http://dx.doi.org/10.1155/2014/492872 |
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