Effects on Proliferation and Differentiation of Human Umbilical Cord-Derived Mesenchymal Stem Cells Engineered to Express Neurotrophic Factors
Mesenchymal stem cells (MSCs) are multipotential cells with capability to form colonies in vitro and differentiate into distinctive end-stage cell types. Although MSCs secrete many cytokines, the efficacy can be improved through combination with neurotrophic factors (NTFs). Moreover, MSCs are excell...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2016-01-01
|
| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2016/1801340 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850163802494992384 |
|---|---|
| author | Yi Wang Youguo Ying Xiaoyan Cui |
| author_facet | Yi Wang Youguo Ying Xiaoyan Cui |
| author_sort | Yi Wang |
| collection | DOAJ |
| description | Mesenchymal stem cells (MSCs) are multipotential cells with capability to form colonies in vitro and differentiate into distinctive end-stage cell types. Although MSCs secrete many cytokines, the efficacy can be improved through combination with neurotrophic factors (NTFs). Moreover, MSCs are excellent opportunities for local delivery of NTFs into injured tissues. The aim of this present study is to evaluate the effects of overexpressing NTFs on proliferation and differentiation of human umbilical cord-derived mesenchymal stem cells (HUMSCs). Overexpressing NTFs had no effect on cell proliferation. Overexpressing NT-3, BDNF, and NGF also had no significant effect on the differentiation of HUMSCs. Overexpressing NTFs all promoted the neurite outgrowth of embryonic chick E9 dorsal root ganglion (DRG). The gene expression profiles of the control and NT-3- and BDNF-modified HUMSCs were compared using RNA sequencing and biological processes and activities were revealed. This study provides novel information about the effects of overexpressing NTFs on HUMSCs and insight into the choice of optimal NTFs for combined cell and gene therapy. |
| format | Article |
| id | doaj-art-ed1c9012ebbc4e7ea739953fa564ec42 |
| institution | OA Journals |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-ed1c9012ebbc4e7ea739953fa564ec422025-08-20T02:22:09ZengWileyStem Cells International1687-966X1687-96782016-01-01201610.1155/2016/18013401801340Effects on Proliferation and Differentiation of Human Umbilical Cord-Derived Mesenchymal Stem Cells Engineered to Express Neurotrophic FactorsYi Wang0Youguo Ying1Xiaoyan Cui2Department of Orthopaedics, Tongren Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200336, ChinaDepartment of Intensive Care Unit, Shanghai 9th People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 201999, ChinaStem Cell Research Center, Tongji University School of Medicine, Shanghai 200092, ChinaMesenchymal stem cells (MSCs) are multipotential cells with capability to form colonies in vitro and differentiate into distinctive end-stage cell types. Although MSCs secrete many cytokines, the efficacy can be improved through combination with neurotrophic factors (NTFs). Moreover, MSCs are excellent opportunities for local delivery of NTFs into injured tissues. The aim of this present study is to evaluate the effects of overexpressing NTFs on proliferation and differentiation of human umbilical cord-derived mesenchymal stem cells (HUMSCs). Overexpressing NTFs had no effect on cell proliferation. Overexpressing NT-3, BDNF, and NGF also had no significant effect on the differentiation of HUMSCs. Overexpressing NTFs all promoted the neurite outgrowth of embryonic chick E9 dorsal root ganglion (DRG). The gene expression profiles of the control and NT-3- and BDNF-modified HUMSCs were compared using RNA sequencing and biological processes and activities were revealed. This study provides novel information about the effects of overexpressing NTFs on HUMSCs and insight into the choice of optimal NTFs for combined cell and gene therapy.http://dx.doi.org/10.1155/2016/1801340 |
| spellingShingle | Yi Wang Youguo Ying Xiaoyan Cui Effects on Proliferation and Differentiation of Human Umbilical Cord-Derived Mesenchymal Stem Cells Engineered to Express Neurotrophic Factors Stem Cells International |
| title | Effects on Proliferation and Differentiation of Human Umbilical Cord-Derived Mesenchymal Stem Cells Engineered to Express Neurotrophic Factors |
| title_full | Effects on Proliferation and Differentiation of Human Umbilical Cord-Derived Mesenchymal Stem Cells Engineered to Express Neurotrophic Factors |
| title_fullStr | Effects on Proliferation and Differentiation of Human Umbilical Cord-Derived Mesenchymal Stem Cells Engineered to Express Neurotrophic Factors |
| title_full_unstemmed | Effects on Proliferation and Differentiation of Human Umbilical Cord-Derived Mesenchymal Stem Cells Engineered to Express Neurotrophic Factors |
| title_short | Effects on Proliferation and Differentiation of Human Umbilical Cord-Derived Mesenchymal Stem Cells Engineered to Express Neurotrophic Factors |
| title_sort | effects on proliferation and differentiation of human umbilical cord derived mesenchymal stem cells engineered to express neurotrophic factors |
| url | http://dx.doi.org/10.1155/2016/1801340 |
| work_keys_str_mv | AT yiwang effectsonproliferationanddifferentiationofhumanumbilicalcordderivedmesenchymalstemcellsengineeredtoexpressneurotrophicfactors AT youguoying effectsonproliferationanddifferentiationofhumanumbilicalcordderivedmesenchymalstemcellsengineeredtoexpressneurotrophicfactors AT xiaoyancui effectsonproliferationanddifferentiationofhumanumbilicalcordderivedmesenchymalstemcellsengineeredtoexpressneurotrophicfactors |