Opposing roles for Bmp signalling during the development of electrosensory lateral line organs
The lateral line system enables fishes and aquatic-stage amphibians to detect local water movement via mechanosensory hair cells in neuromasts, and many species to detect weak electric fields via electroreceptors (modified hair cells) in ampullary organs. Both neuromasts and ampullary organs develop...
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eLife Sciences Publications Ltd
2025-01-01
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| Online Access: | https://elifesciences.org/articles/99798 |
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| author | Alexander S Campbell Martin Minařík Roman Franěk Michaela Vazačová Miloš Havelka David Gela Martin Pšenička Clare VH Baker |
| author_facet | Alexander S Campbell Martin Minařík Roman Franěk Michaela Vazačová Miloš Havelka David Gela Martin Pšenička Clare VH Baker |
| author_sort | Alexander S Campbell |
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| description | The lateral line system enables fishes and aquatic-stage amphibians to detect local water movement via mechanosensory hair cells in neuromasts, and many species to detect weak electric fields via electroreceptors (modified hair cells) in ampullary organs. Both neuromasts and ampullary organs develop from lateral line placodes, but the molecular mechanisms underpinning ampullary organ formation are understudied relative to neuromasts. This is because the ancestral lineages of zebrafish (teleosts) and Xenopus (frogs) independently lost electroreception. We identified Bmp5 as a promising candidate via differential RNA-seq in an electroreceptive ray-finned fish, the Mississippi paddlefish (Polyodon spathula; Modrell et al., 2017, eLife 6: e24197). In an experimentally tractable relative, the sterlet sturgeon (Acipenser ruthenus), we found that Bmp5 and four other Bmp pathway genes are expressed in the developing lateral line, and that Bmp signalling is active. Furthermore, CRISPR/Cas9-mediated mutagenesis targeting Bmp5 in G0-injected sterlet embryos resulted in fewer ampullary organs. Conversely, when Bmp signalling was inhibited by DMH1 treatment shortly before the formation of ampullary organ primordia, supernumerary ampullary organs developed. These data suggest that Bmp5 promotes ampullary organ development, whereas Bmp signalling via another ligand(s) prevents their overproduction. Taken together, this demonstrates opposing roles for Bmp signalling during ampullary organ formation. |
| format | Article |
| id | doaj-art-ed135d18d90a4bf4b7ae43862d4b0f0e |
| institution | DOAJ |
| issn | 2050-084X |
| language | English |
| publishDate | 2025-01-01 |
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| spelling | doaj-art-ed135d18d90a4bf4b7ae43862d4b0f0e2025-08-20T02:42:52ZengeLife Sciences Publications LtdeLife2050-084X2025-01-011410.7554/eLife.99798Opposing roles for Bmp signalling during the development of electrosensory lateral line organsAlexander S Campbell0https://orcid.org/0009-0003-1539-214XMartin Minařík1https://orcid.org/0000-0001-6660-0031Roman Franěk2https://orcid.org/0000-0002-3464-1872Michaela Vazačová3Miloš Havelka4David Gela5Martin Pšenička6Clare VH Baker7https://orcid.org/0000-0002-4434-3107Department of Physiology, Development & Neuroscience, University of Cambridge, Cambridge, United KingdomDepartment of Physiology, Development & Neuroscience, University of Cambridge, Cambridge, United KingdomFaculty of Fisheries and Protection of Waters, Research Institute of Fish Culture and Hydrobiology, University of South Bohemia in České Budějovice, Vodňany, Czech RepublicFaculty of Fisheries and Protection of Waters, Research Institute of Fish Culture and Hydrobiology, University of South Bohemia in České Budějovice, Vodňany, Czech RepublicFaculty of Fisheries and Protection of Waters, Research Institute of Fish Culture and Hydrobiology, University of South Bohemia in České Budějovice, Vodňany, Czech RepublicFaculty of Fisheries and Protection of Waters, Research Institute of Fish Culture and Hydrobiology, University of South Bohemia in České Budějovice, Vodňany, Czech RepublicFaculty of Fisheries and Protection of Waters, Research Institute of Fish Culture and Hydrobiology, University of South Bohemia in České Budějovice, Vodňany, Czech RepublicDepartment of Physiology, Development & Neuroscience, University of Cambridge, Cambridge, United KingdomThe lateral line system enables fishes and aquatic-stage amphibians to detect local water movement via mechanosensory hair cells in neuromasts, and many species to detect weak electric fields via electroreceptors (modified hair cells) in ampullary organs. Both neuromasts and ampullary organs develop from lateral line placodes, but the molecular mechanisms underpinning ampullary organ formation are understudied relative to neuromasts. This is because the ancestral lineages of zebrafish (teleosts) and Xenopus (frogs) independently lost electroreception. We identified Bmp5 as a promising candidate via differential RNA-seq in an electroreceptive ray-finned fish, the Mississippi paddlefish (Polyodon spathula; Modrell et al., 2017, eLife 6: e24197). In an experimentally tractable relative, the sterlet sturgeon (Acipenser ruthenus), we found that Bmp5 and four other Bmp pathway genes are expressed in the developing lateral line, and that Bmp signalling is active. Furthermore, CRISPR/Cas9-mediated mutagenesis targeting Bmp5 in G0-injected sterlet embryos resulted in fewer ampullary organs. Conversely, when Bmp signalling was inhibited by DMH1 treatment shortly before the formation of ampullary organ primordia, supernumerary ampullary organs developed. These data suggest that Bmp5 promotes ampullary organ development, whereas Bmp signalling via another ligand(s) prevents their overproduction. Taken together, this demonstrates opposing roles for Bmp signalling during ampullary organ formation.https://elifesciences.org/articles/99798lateral lineampullary organsneuromastsBmpsterlet sturgeonAcipenser ruthenus |
| spellingShingle | Alexander S Campbell Martin Minařík Roman Franěk Michaela Vazačová Miloš Havelka David Gela Martin Pšenička Clare VH Baker Opposing roles for Bmp signalling during the development of electrosensory lateral line organs eLife lateral line ampullary organs neuromasts Bmp sterlet sturgeon Acipenser ruthenus |
| title | Opposing roles for Bmp signalling during the development of electrosensory lateral line organs |
| title_full | Opposing roles for Bmp signalling during the development of electrosensory lateral line organs |
| title_fullStr | Opposing roles for Bmp signalling during the development of electrosensory lateral line organs |
| title_full_unstemmed | Opposing roles for Bmp signalling during the development of electrosensory lateral line organs |
| title_short | Opposing roles for Bmp signalling during the development of electrosensory lateral line organs |
| title_sort | opposing roles for bmp signalling during the development of electrosensory lateral line organs |
| topic | lateral line ampullary organs neuromasts Bmp sterlet sturgeon Acipenser ruthenus |
| url | https://elifesciences.org/articles/99798 |
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