Combination Effect of N-Hexane Extract of Plectranthus amboinicus (Lour.) Spreng. with Doxorubicin Againts HeLa Cell Lines
The study was aimed to investigate the growth inhibiting effect of Plectranthus amboinicus, (Lour.) Spreng n-hexane extract (PAN) in combination with doxorubicin againts HeLa cell Lines, to observe the apoptotic induction and immunocytochemistry of HeLa cell Lines on cyclin D1, Bcl-2, and Cox-2 afte...
Saved in:
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Indonesian Society for Cancer Chemoprevention
2015-10-01
|
| Series: | ISCC (Indonesian Journal of Cancer Chemoprevention) |
| Online Access: | https://ijcc.chemoprev.org/index.php/ijcc/article/view/94 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849323357305044992 |
|---|---|
| author | Poppy Anjelisa Z. Hasibuan Rosidah Rosidah |
| author_facet | Poppy Anjelisa Z. Hasibuan Rosidah Rosidah |
| author_sort | Poppy Anjelisa Z. Hasibuan |
| collection | DOAJ |
| description | The study was aimed to investigate the growth inhibiting effect of Plectranthus amboinicus, (Lour.) Spreng n-hexane extract (PAN) in combination with doxorubicin againts HeLa cell Lines, to observe the apoptotic induction and immunocytochemistry of HeLa cell Lines on cyclin D1, Bcl-2, and Cox-2 after treatment of PAN. The percentage viability of the cell were carried out by using MTT [3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyl tetrazolium bromide] assay. The effect of apoptosis was observed by flowcytometry assay in single dose of PAN. The expression of cyclin D1, Bcl2 and COX-2 proteins were investigated on HeLa cell lines by using immunocytochemistry. The result showed that PAN showed strong synergistic effect with doxorubicin againts HeLa cells based on Combination Index analysis. No significant apoptotic induction of PAN in HeLa cell lines, but the extract caused necrosis. The immunocytochemical study showed suppression of cyclin D1, Bcl2 and COX-2 expression on HeLa cell lines. The results concluded that PAN could be a potential co-chemoterapeutic agent with doxorubicin on cervix cancer cells but need to be explored further by its combination on specific molecular target.
Keywords : Plectranthus amboinicus, (Lour.) Spreng., combination, HeLa cell lines |
| format | Article |
| id | doaj-art-ed0cae9fd59246be82e62caf3088ec24 |
| institution | Kabale University |
| issn | 2088-0197 2355-8989 |
| language | English |
| publishDate | 2015-10-01 |
| publisher | Indonesian Society for Cancer Chemoprevention |
| record_format | Article |
| series | ISCC (Indonesian Journal of Cancer Chemoprevention) |
| spelling | doaj-art-ed0cae9fd59246be82e62caf3088ec242025-08-20T03:49:03ZengIndonesian Society for Cancer ChemopreventionISCC (Indonesian Journal of Cancer Chemoprevention)2088-01972355-89892015-10-016311111510.14499/indonesianjcanchemoprev6iss3pp111-11595Combination Effect of N-Hexane Extract of Plectranthus amboinicus (Lour.) Spreng. with Doxorubicin Againts HeLa Cell LinesPoppy Anjelisa Z. Hasibuan0Rosidah Rosidah1Faculty of Pharmacy, Universitas Sumatera Utara, MedanFaculty of Pharmacy, Universitas Sumatera Utara, MedanThe study was aimed to investigate the growth inhibiting effect of Plectranthus amboinicus, (Lour.) Spreng n-hexane extract (PAN) in combination with doxorubicin againts HeLa cell Lines, to observe the apoptotic induction and immunocytochemistry of HeLa cell Lines on cyclin D1, Bcl-2, and Cox-2 after treatment of PAN. The percentage viability of the cell were carried out by using MTT [3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyl tetrazolium bromide] assay. The effect of apoptosis was observed by flowcytometry assay in single dose of PAN. The expression of cyclin D1, Bcl2 and COX-2 proteins were investigated on HeLa cell lines by using immunocytochemistry. The result showed that PAN showed strong synergistic effect with doxorubicin againts HeLa cells based on Combination Index analysis. No significant apoptotic induction of PAN in HeLa cell lines, but the extract caused necrosis. The immunocytochemical study showed suppression of cyclin D1, Bcl2 and COX-2 expression on HeLa cell lines. The results concluded that PAN could be a potential co-chemoterapeutic agent with doxorubicin on cervix cancer cells but need to be explored further by its combination on specific molecular target. Keywords : Plectranthus amboinicus, (Lour.) Spreng., combination, HeLa cell lineshttps://ijcc.chemoprev.org/index.php/ijcc/article/view/94 |
| spellingShingle | Poppy Anjelisa Z. Hasibuan Rosidah Rosidah Combination Effect of N-Hexane Extract of Plectranthus amboinicus (Lour.) Spreng. with Doxorubicin Againts HeLa Cell Lines ISCC (Indonesian Journal of Cancer Chemoprevention) |
| title | Combination Effect of N-Hexane Extract of Plectranthus amboinicus (Lour.) Spreng. with Doxorubicin Againts HeLa Cell Lines |
| title_full | Combination Effect of N-Hexane Extract of Plectranthus amboinicus (Lour.) Spreng. with Doxorubicin Againts HeLa Cell Lines |
| title_fullStr | Combination Effect of N-Hexane Extract of Plectranthus amboinicus (Lour.) Spreng. with Doxorubicin Againts HeLa Cell Lines |
| title_full_unstemmed | Combination Effect of N-Hexane Extract of Plectranthus amboinicus (Lour.) Spreng. with Doxorubicin Againts HeLa Cell Lines |
| title_short | Combination Effect of N-Hexane Extract of Plectranthus amboinicus (Lour.) Spreng. with Doxorubicin Againts HeLa Cell Lines |
| title_sort | combination effect of n hexane extract of plectranthus amboinicus lour spreng with doxorubicin againts hela cell lines |
| url | https://ijcc.chemoprev.org/index.php/ijcc/article/view/94 |
| work_keys_str_mv | AT poppyanjelisazhasibuan combinationeffectofnhexaneextractofplectranthusamboinicusloursprengwithdoxorubicinagaintshelacelllines AT rosidahrosidah combinationeffectofnhexaneextractofplectranthusamboinicusloursprengwithdoxorubicinagaintshelacelllines |