Bioinformatics and Functional Validation of <i>CqPRX9L1</i> in <i>Chenopodium quinoa</i>
As a plant-specific peroxidase family, class III peroxidase (PRX) plays an important role in plant growth, development, and stress response. In this study, a preliminary functional analysis of <i>CqPRX9L1</i> was conducted. Bioinformatics analysis revealed that <i>CqPRX9L1</i>...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-07-01
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| Series: | Plants |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2223-7747/14/14/2246 |
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| Summary: | As a plant-specific peroxidase family, class III peroxidase (PRX) plays an important role in plant growth, development, and stress response. In this study, a preliminary functional analysis of <i>CqPRX9L1</i> was conducted. Bioinformatics analysis revealed that <i>CqPRX9L1</i> encodes a 349-amino acid protein belonging to the plant-peroxidase-like superfamily, featuring a transmembrane domain and cytoplasmic localization. The promoter region of <i>CqPRX9L1</i> harbors various cis-acting elements associated with stress responses, hormone signaling, light regulation, and meristem-specific expression. The tissue-specific expression pattern of the <i>CqPRX9L1</i> gene and its characteristics in response to different stresses were explored using subcellular localization, quantitative real-time PCR (qRT-PCR), and heterologous transformation into <i>Arabidopsis thaliana.</i> The results showed that CqPRX9L1, with a transmembrane structure, was localized in the cytoplasm, which encodes 349 amino acids and belongs to the plant-peroxisome-like superfamily. The promoter region contains stress-response elements, hormone-response elements, light-response elements, and meristem expression-related elements. The expression of <i>CqPRX9L1</i> was relatively higher in ears and roots at the panicle stage than in stems and leaves. <i>CqPRX9L1</i> showed a dynamic expression pattern of first decreasing and then increasing under abiotic stresses such as 15% PEG 6000, low temperature, and salt damage, with differences in response time and degree. <i>CqPRX9L1</i> plays an important role in response to abiotic stress by affecting the activity of antioxidant enzymes such as superoxide dismutase (SOD) and peroxidase (POD), as well as the synthesis and decomposition of proline (Pro). <i>CqPRX9L1</i> also affects plant bolting and flowering by regulating key flowering genes (such as FT and AP1) and gibberellin (GA)-related pathways. The results establish a foundation for revealing the functions and molecular mechanisms of the <i>CqPRX9L1</i> gene. |
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| ISSN: | 2223-7747 |