CDCA genes as prognostic and therapeutic targets in Colon adenocarcinoma
Abstract Objectives The study investigates the role of Cell Division Cycle Associated (CDCA) genes in colorectal cancer (COAD) by analyzing their differential expression, epigenetic alterations, prognostic significance, and functional associations. Methodology This study employed a detailed in silic...
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| Format: | Article |
| Language: | English |
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BMC
2025-02-01
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| Series: | Hereditas |
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| Online Access: | https://doi.org/10.1186/s41065-025-00368-w |
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| author | Zongquan Zhao Xinwei Feng Bo Chen Yihong Wu Xiaohong Wang Zhenyuan Tang Min Huang Xiaohua Guo |
| author_facet | Zongquan Zhao Xinwei Feng Bo Chen Yihong Wu Xiaohong Wang Zhenyuan Tang Min Huang Xiaohua Guo |
| author_sort | Zongquan Zhao |
| collection | DOAJ |
| description | Abstract Objectives The study investigates the role of Cell Division Cycle Associated (CDCA) genes in colorectal cancer (COAD) by analyzing their differential expression, epigenetic alterations, prognostic significance, and functional associations. Methodology This study employed a detailed in silico and in vitro experiments-based methodology. Results RT-qPCR assays reveal significantly elevated mRNA levels of CDCA2, CDCA3, CDCA4, CDCA5, CDCA7, and CDCA8 genes in COAD cell lines compared to controls. Bisulfite sequencing indicates reduced promoter methylation of CDCA gene promoters in COAD cell lines, suggesting an epigenetic regulatory mechanism. Analysis of large TCGA datasets confirms increased CDCA gene expression in COAD tissues. Survival analysis using cSurvival database demonstrates negative correlations between CDCA gene expression and patient overall survival. Additionally, Lasso regression-based models of CDCA genes predict survival outcomes in COAD patients. Investigating immune modulation, CDCA gene expression inversely correlates with immune cell infiltration and immune modulators. miRNA-mRNA network analysis identifies regulatory miRNAs targeting CDCA genes, validated by RT-qPCR showing up-regulation of has-mir-10a-5p and has-mir-20a-5p in COAD cell lines and tissues. Drug sensitivity analysis suggests resistance to specific drugs in COAD patients with elevated CDCA gene expression. Furthermore, CDCA gene expression correlates with crucial functional states in COAD, including “angiogenesis, apoptosis, differentiation, hypoxia, inflammation, and metastasis.” Additional in vitro experiments revealed that CDCA2 and CDCA3 knockdown in SW480 and SW629 cells significantly reduced cell proliferation and colony formation while enhancing cell migration. Conclusion Overall, the study elucidates the multifaceted role of CDCA genes in COAD progression, providing insights into potential diagnostic, prognostic, and therapeutic implications. |
| format | Article |
| id | doaj-art-ed01720d76d840b6b0573bc3406854ac |
| institution | OA Journals |
| issn | 1601-5223 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | BMC |
| record_format | Article |
| series | Hereditas |
| spelling | doaj-art-ed01720d76d840b6b0573bc3406854ac2025-08-20T02:13:06ZengBMCHereditas1601-52232025-02-01162112010.1186/s41065-025-00368-wCDCA genes as prognostic and therapeutic targets in Colon adenocarcinomaZongquan Zhao0Xinwei Feng1Bo Chen2Yihong Wu3Xiaohong Wang4Zhenyuan Tang5Min Huang6Xiaohua Guo7Department of General PracticeDepartment of Digestive Internal Medicine, Shanghai Changzheng HospitalDepartment of Oncology, Chengdu First People’s HospitalDepartment of General Practice, Runda Community Health Service CenterDepartment of General PracticeDepartment of General Practice, Community Health Management Center of Suzhou Municipal HospitalDepartment of General Practice, Suzhou Municipal HospitalDepartment of Digestive Surgery, Xi’an Jiaotong University School of Medicine Affiliated Honghui HospitalAbstract Objectives The study investigates the role of Cell Division Cycle Associated (CDCA) genes in colorectal cancer (COAD) by analyzing their differential expression, epigenetic alterations, prognostic significance, and functional associations. Methodology This study employed a detailed in silico and in vitro experiments-based methodology. Results RT-qPCR assays reveal significantly elevated mRNA levels of CDCA2, CDCA3, CDCA4, CDCA5, CDCA7, and CDCA8 genes in COAD cell lines compared to controls. Bisulfite sequencing indicates reduced promoter methylation of CDCA gene promoters in COAD cell lines, suggesting an epigenetic regulatory mechanism. Analysis of large TCGA datasets confirms increased CDCA gene expression in COAD tissues. Survival analysis using cSurvival database demonstrates negative correlations between CDCA gene expression and patient overall survival. Additionally, Lasso regression-based models of CDCA genes predict survival outcomes in COAD patients. Investigating immune modulation, CDCA gene expression inversely correlates with immune cell infiltration and immune modulators. miRNA-mRNA network analysis identifies regulatory miRNAs targeting CDCA genes, validated by RT-qPCR showing up-regulation of has-mir-10a-5p and has-mir-20a-5p in COAD cell lines and tissues. Drug sensitivity analysis suggests resistance to specific drugs in COAD patients with elevated CDCA gene expression. Furthermore, CDCA gene expression correlates with crucial functional states in COAD, including “angiogenesis, apoptosis, differentiation, hypoxia, inflammation, and metastasis.” Additional in vitro experiments revealed that CDCA2 and CDCA3 knockdown in SW480 and SW629 cells significantly reduced cell proliferation and colony formation while enhancing cell migration. Conclusion Overall, the study elucidates the multifaceted role of CDCA genes in COAD progression, providing insights into potential diagnostic, prognostic, and therapeutic implications.https://doi.org/10.1186/s41065-025-00368-wCOADCDCA genesBiomarkerPrognosisTreatmentDiagnosis |
| spellingShingle | Zongquan Zhao Xinwei Feng Bo Chen Yihong Wu Xiaohong Wang Zhenyuan Tang Min Huang Xiaohua Guo CDCA genes as prognostic and therapeutic targets in Colon adenocarcinoma Hereditas COAD CDCA genes Biomarker Prognosis Treatment Diagnosis |
| title | CDCA genes as prognostic and therapeutic targets in Colon adenocarcinoma |
| title_full | CDCA genes as prognostic and therapeutic targets in Colon adenocarcinoma |
| title_fullStr | CDCA genes as prognostic and therapeutic targets in Colon adenocarcinoma |
| title_full_unstemmed | CDCA genes as prognostic and therapeutic targets in Colon adenocarcinoma |
| title_short | CDCA genes as prognostic and therapeutic targets in Colon adenocarcinoma |
| title_sort | cdca genes as prognostic and therapeutic targets in colon adenocarcinoma |
| topic | COAD CDCA genes Biomarker Prognosis Treatment Diagnosis |
| url | https://doi.org/10.1186/s41065-025-00368-w |
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