Waste management and disease spread potential: A case study of SARS-CoV-2 in garbage dumping sites in Bangkok and its vicinity

During the coronavirus disease 2019 (COVID-19) pandemic, hospitals and households have used personal protective equipment (PPE), such as masks and gloves. Some of these potentially infectious materials were discarded with other household wastes in garbage dumping sites. Thus, this study aimed to det...

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Main Authors: Anuwat Wiratsudakul, Ladawan Sariya, Weena Paungpin, Sarin Suwanpakdee, Tatiyanuch Chamsai, Siriporn Tangsudjai, Benjaporn Bhusri, Peerawat Wongluechai, Kanittha Tonchiangsai, Walasinee Sakcamduang, Witthawat Wiriyarat, Nareerat Sangkachai
Format: Article
Language:English
Published: Elsevier 2024-12-01
Series:One Health
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Online Access:http://www.sciencedirect.com/science/article/pii/S2352771424002209
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author Anuwat Wiratsudakul
Ladawan Sariya
Weena Paungpin
Sarin Suwanpakdee
Tatiyanuch Chamsai
Siriporn Tangsudjai
Benjaporn Bhusri
Peerawat Wongluechai
Kanittha Tonchiangsai
Walasinee Sakcamduang
Witthawat Wiriyarat
Nareerat Sangkachai
author_facet Anuwat Wiratsudakul
Ladawan Sariya
Weena Paungpin
Sarin Suwanpakdee
Tatiyanuch Chamsai
Siriporn Tangsudjai
Benjaporn Bhusri
Peerawat Wongluechai
Kanittha Tonchiangsai
Walasinee Sakcamduang
Witthawat Wiriyarat
Nareerat Sangkachai
author_sort Anuwat Wiratsudakul
collection DOAJ
description During the coronavirus disease 2019 (COVID-19) pandemic, hospitals and households have used personal protective equipment (PPE), such as masks and gloves. Some of these potentially infectious materials were discarded with other household wastes in garbage dumping sites. Thus, this study aimed to detect the presence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in contaminated wastes, environments, and mammals scavenging around these sites. From September to October 2022, we visited three garbage dumping sites located in Bangkok, Nakhon Pathom, and Nonthaburi provinces of Thailand. Oral, nasal, rectal swabs, and blood samples were collected from small mammals, stray dogs, and cats. Masks, gloves, soil, and water samples from the sites were additionally collected. Of the 582 samples collected from 238 animals, none tested positive for SARS-CoV-2 in the virus isolation, real-time reverse-transcription polymerase chain reaction, and neutralizing antibody detection. However, one sample (1.18 %; 1/85) from a rat (Rattus spp.) captured in Nonthaburi was serologically positive in the indirect enzyme-linked immunosorbent assay. The surveillance of coronaviruses in rats is strongly encouraged because rats may harbor different zoonotic pathogens, including unknown potentially zoonotic coronaviruses. Moreover, two face mask samples (4.65 %; 2/43) collected from the dumping site in Nakhon Pathom tested positive for SARS-CoV-2 by real-time RT-PCR. To reduce environmental contamination, detecting the SARS-CoV-2 viral genome in contaminated face masks highlights the critical need for proper waste management in households and communities in Thailand. Thus, to minimize exposure and prevent onward transmission, waste management personnel, including garbage dump staff and waste pickers, should be equipped with appropriate PPE and receive regular training on safe handling and disposal.
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spelling doaj-art-ecfa8bd72775433eb345ff0d7d579d182025-08-20T02:36:58ZengElsevierOne Health2352-77142024-12-011910089410.1016/j.onehlt.2024.100894Waste management and disease spread potential: A case study of SARS-CoV-2 in garbage dumping sites in Bangkok and its vicinityAnuwat Wiratsudakul0Ladawan Sariya1Weena Paungpin2Sarin Suwanpakdee3Tatiyanuch Chamsai4Siriporn Tangsudjai5Benjaporn Bhusri6Peerawat Wongluechai7Kanittha Tonchiangsai8Walasinee Sakcamduang9Witthawat Wiriyarat10Nareerat Sangkachai11Department of Clinical Sciences and Public Health, Faculty of Veterinary Science, Mahidol University, Salaya, Nakhon Pathom, Thailand; The Monitoring and Surveillance Center for Zoonotic Diseases in Wildlife and Exotic Animals, Faculty of Veterinary Science, Mahidol University, Salaya, Nakhon Pathom, ThailandThe Monitoring and Surveillance Center for Zoonotic Diseases in Wildlife and Exotic Animals, Faculty of Veterinary Science, Mahidol University, Salaya, Nakhon Pathom, ThailandThe Monitoring and Surveillance Center for Zoonotic Diseases in Wildlife and Exotic Animals, Faculty of Veterinary Science, Mahidol University, Salaya, Nakhon Pathom, ThailandDepartment of Clinical Sciences and Public Health, Faculty of Veterinary Science, Mahidol University, Salaya, Nakhon Pathom, Thailand; The Monitoring and Surveillance Center for Zoonotic Diseases in Wildlife and Exotic Animals, Faculty of Veterinary Science, Mahidol University, Salaya, Nakhon Pathom, ThailandThe Monitoring and Surveillance Center for Zoonotic Diseases in Wildlife and Exotic Animals, Faculty of Veterinary Science, Mahidol University, Salaya, Nakhon Pathom, ThailandThe Monitoring and Surveillance Center for Zoonotic Diseases in Wildlife and Exotic Animals, Faculty of Veterinary Science, Mahidol University, Salaya, Nakhon Pathom, ThailandThe Monitoring and Surveillance Center for Zoonotic Diseases in Wildlife and Exotic Animals, Faculty of Veterinary Science, Mahidol University, Salaya, Nakhon Pathom, ThailandThe Monitoring and Surveillance Center for Zoonotic Diseases in Wildlife and Exotic Animals, Faculty of Veterinary Science, Mahidol University, Salaya, Nakhon Pathom, ThailandThe Monitoring and Surveillance Center for Zoonotic Diseases in Wildlife and Exotic Animals, Faculty of Veterinary Science, Mahidol University, Salaya, Nakhon Pathom, ThailandDepartment of Clinical Sciences and Public Health, Faculty of Veterinary Science, Mahidol University, Salaya, Nakhon Pathom, ThailandThe Monitoring and Surveillance Center for Zoonotic Diseases in Wildlife and Exotic Animals, Faculty of Veterinary Science, Mahidol University, Salaya, Nakhon Pathom, Thailand; Department of Pre-clinic and Applied Animal Science, Faculty of Veterinary Science, Mahidol University, Salaya, Nakhon Pathom, ThailandThe Monitoring and Surveillance Center for Zoonotic Diseases in Wildlife and Exotic Animals, Faculty of Veterinary Science, Mahidol University, Salaya, Nakhon Pathom, Thailand; Corresponding author.During the coronavirus disease 2019 (COVID-19) pandemic, hospitals and households have used personal protective equipment (PPE), such as masks and gloves. Some of these potentially infectious materials were discarded with other household wastes in garbage dumping sites. Thus, this study aimed to detect the presence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in contaminated wastes, environments, and mammals scavenging around these sites. From September to October 2022, we visited three garbage dumping sites located in Bangkok, Nakhon Pathom, and Nonthaburi provinces of Thailand. Oral, nasal, rectal swabs, and blood samples were collected from small mammals, stray dogs, and cats. Masks, gloves, soil, and water samples from the sites were additionally collected. Of the 582 samples collected from 238 animals, none tested positive for SARS-CoV-2 in the virus isolation, real-time reverse-transcription polymerase chain reaction, and neutralizing antibody detection. However, one sample (1.18 %; 1/85) from a rat (Rattus spp.) captured in Nonthaburi was serologically positive in the indirect enzyme-linked immunosorbent assay. The surveillance of coronaviruses in rats is strongly encouraged because rats may harbor different zoonotic pathogens, including unknown potentially zoonotic coronaviruses. Moreover, two face mask samples (4.65 %; 2/43) collected from the dumping site in Nakhon Pathom tested positive for SARS-CoV-2 by real-time RT-PCR. To reduce environmental contamination, detecting the SARS-CoV-2 viral genome in contaminated face masks highlights the critical need for proper waste management in households and communities in Thailand. Thus, to minimize exposure and prevent onward transmission, waste management personnel, including garbage dump staff and waste pickers, should be equipped with appropriate PPE and receive regular training on safe handling and disposal.http://www.sciencedirect.com/science/article/pii/S2352771424002209Face maskGarbage dumping siteSARS-CoV-2SerologySmall mammals
spellingShingle Anuwat Wiratsudakul
Ladawan Sariya
Weena Paungpin
Sarin Suwanpakdee
Tatiyanuch Chamsai
Siriporn Tangsudjai
Benjaporn Bhusri
Peerawat Wongluechai
Kanittha Tonchiangsai
Walasinee Sakcamduang
Witthawat Wiriyarat
Nareerat Sangkachai
Waste management and disease spread potential: A case study of SARS-CoV-2 in garbage dumping sites in Bangkok and its vicinity
One Health
Face mask
Garbage dumping site
SARS-CoV-2
Serology
Small mammals
title Waste management and disease spread potential: A case study of SARS-CoV-2 in garbage dumping sites in Bangkok and its vicinity
title_full Waste management and disease spread potential: A case study of SARS-CoV-2 in garbage dumping sites in Bangkok and its vicinity
title_fullStr Waste management and disease spread potential: A case study of SARS-CoV-2 in garbage dumping sites in Bangkok and its vicinity
title_full_unstemmed Waste management and disease spread potential: A case study of SARS-CoV-2 in garbage dumping sites in Bangkok and its vicinity
title_short Waste management and disease spread potential: A case study of SARS-CoV-2 in garbage dumping sites in Bangkok and its vicinity
title_sort waste management and disease spread potential a case study of sars cov 2 in garbage dumping sites in bangkok and its vicinity
topic Face mask
Garbage dumping site
SARS-CoV-2
Serology
Small mammals
url http://www.sciencedirect.com/science/article/pii/S2352771424002209
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