Xenotransplantation of Cryopreserved Calf Testicular Tissues
The cryopreservation of testicular tissues meets the demands for the germplasm preservation of humans and animals. Previously, we reported on the cryopreservation of bovine testicular tissues. To further evaluate the viability of these tissues, subcutaneous xenotransplantation of the frozen–thawed c...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-03-01
|
| Series: | Veterinary Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2306-7381/12/3/247 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850277471237177344 |
|---|---|
| author | Yansen Zhao Wenqian Zhu Rui Yang Boyang Zhang Bo Tang Xueming Zhang |
| author_facet | Yansen Zhao Wenqian Zhu Rui Yang Boyang Zhang Bo Tang Xueming Zhang |
| author_sort | Yansen Zhao |
| collection | DOAJ |
| description | The cryopreservation of testicular tissues meets the demands for the germplasm preservation of humans and animals. Previously, we reported on the cryopreservation of bovine testicular tissues. To further evaluate the viability of these tissues, subcutaneous xenotransplantation of the frozen–thawed calf testicular tissues was performed with castrated nude mice as the recipients. After 28 days (D28), the survival and development of the grafts were examined. The grafts from 1-day-old (D1) calf testes were recovered and angiogenesis around the grafts was observed. Histologically, the seminiferous cords in the grafts were well maintained and capillaries in the interstitium were observed. Quantitative real-time PCR (qRT-PCR) analysis showed that the grafts expressed germline genes <i>Gfrα-1</i>, <i>C-kit</i>, and <i>Sycp3</i> and somatic genes <i>Sox9</i>, <i>Acta2,</i> and <i>Star</i>. The expressions of <i>C-kit</i>, <i>Sox9</i>, <i>Acta2</i>, and Star were higher in 28D grafts than those in 1D and 30-day-old (30D) calf testicular controls. Together, we initially demonstrate that cryopreserved calf testicular tissues retain their viability and developmental capacity after xenotransplantation. |
| format | Article |
| id | doaj-art-ecf748b6e5554d2a8efef39d4371f60f |
| institution | OA Journals |
| issn | 2306-7381 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Veterinary Sciences |
| spelling | doaj-art-ecf748b6e5554d2a8efef39d4371f60f2025-08-20T01:49:53ZengMDPI AGVeterinary Sciences2306-73812025-03-0112324710.3390/vetsci12030247Xenotransplantation of Cryopreserved Calf Testicular TissuesYansen Zhao0Wenqian Zhu1Rui Yang2Boyang Zhang3Bo Tang4Xueming Zhang5State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, College of Veterinary Medicine, Jilin University, Changchun 130062, ChinaState Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, College of Veterinary Medicine, Jilin University, Changchun 130062, ChinaState Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, College of Veterinary Medicine, Jilin University, Changchun 130062, ChinaState Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, College of Veterinary Medicine, Jilin University, Changchun 130062, ChinaState Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, College of Veterinary Medicine, Jilin University, Changchun 130062, ChinaState Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, College of Veterinary Medicine, Jilin University, Changchun 130062, ChinaThe cryopreservation of testicular tissues meets the demands for the germplasm preservation of humans and animals. Previously, we reported on the cryopreservation of bovine testicular tissues. To further evaluate the viability of these tissues, subcutaneous xenotransplantation of the frozen–thawed calf testicular tissues was performed with castrated nude mice as the recipients. After 28 days (D28), the survival and development of the grafts were examined. The grafts from 1-day-old (D1) calf testes were recovered and angiogenesis around the grafts was observed. Histologically, the seminiferous cords in the grafts were well maintained and capillaries in the interstitium were observed. Quantitative real-time PCR (qRT-PCR) analysis showed that the grafts expressed germline genes <i>Gfrα-1</i>, <i>C-kit</i>, and <i>Sycp3</i> and somatic genes <i>Sox9</i>, <i>Acta2,</i> and <i>Star</i>. The expressions of <i>C-kit</i>, <i>Sox9</i>, <i>Acta2</i>, and Star were higher in 28D grafts than those in 1D and 30-day-old (30D) calf testicular controls. Together, we initially demonstrate that cryopreserved calf testicular tissues retain their viability and developmental capacity after xenotransplantation.https://www.mdpi.com/2306-7381/12/3/247cattlefreezing–thawingmouse recipienttestisxenograft |
| spellingShingle | Yansen Zhao Wenqian Zhu Rui Yang Boyang Zhang Bo Tang Xueming Zhang Xenotransplantation of Cryopreserved Calf Testicular Tissues Veterinary Sciences cattle freezing–thawing mouse recipient testis xenograft |
| title | Xenotransplantation of Cryopreserved Calf Testicular Tissues |
| title_full | Xenotransplantation of Cryopreserved Calf Testicular Tissues |
| title_fullStr | Xenotransplantation of Cryopreserved Calf Testicular Tissues |
| title_full_unstemmed | Xenotransplantation of Cryopreserved Calf Testicular Tissues |
| title_short | Xenotransplantation of Cryopreserved Calf Testicular Tissues |
| title_sort | xenotransplantation of cryopreserved calf testicular tissues |
| topic | cattle freezing–thawing mouse recipient testis xenograft |
| url | https://www.mdpi.com/2306-7381/12/3/247 |
| work_keys_str_mv | AT yansenzhao xenotransplantationofcryopreservedcalftesticulartissues AT wenqianzhu xenotransplantationofcryopreservedcalftesticulartissues AT ruiyang xenotransplantationofcryopreservedcalftesticulartissues AT boyangzhang xenotransplantationofcryopreservedcalftesticulartissues AT botang xenotransplantationofcryopreservedcalftesticulartissues AT xuemingzhang xenotransplantationofcryopreservedcalftesticulartissues |