Epitope tagging: a monoclonal antibody specific for recombinant fusion proteins in plants
The easy identification of a recombinant protein in plant material becomes increasingly relevant as more transgenic plants are used for research and commercial applications. Tagging recombinant proteins with a small peptide (epitope) can perform such a task. However, available epitope antibodies wil...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2003-09-01
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| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/03353st01 |
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| _version_ | 1850151928051269632 |
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| author | Susan D. Lawrence Nicole G. Novak Jeffrey M. Slack |
| author_facet | Susan D. Lawrence Nicole G. Novak Jeffrey M. Slack |
| author_sort | Susan D. Lawrence |
| collection | DOAJ |
| description | The easy identification of a recombinant protein in plant material becomes increasingly relevant as more transgenic plants are used for research and commercial applications. Tagging recombinant proteins with a small peptide (epitope) can perform such a task. However, available epitope antibodies will also cross-react with endogenous plant proteins at a level that may be unacceptable. Here we describe the new epitope antibody AcV5. Whether it is attached to the carboxyl terminal end of enhanced green fluorescent protein (EGFP) or the Bacillus thuringiensis endotoxin Cry3A, these proteins remain functional. In addition, using less than 250 pg AcV5-tagged EGFP produces a strong signal on Western blots with no cross-reactivity of proteins from a broad range of plants of agronomic importance. |
| format | Article |
| id | doaj-art-ecf27e656a5242ad841e033bfc010680 |
| institution | OA Journals |
| issn | 0736-6205 1940-9818 |
| language | English |
| publishDate | 2003-09-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | BioTechniques |
| spelling | doaj-art-ecf27e656a5242ad841e033bfc0106802025-08-20T02:26:06ZengTaylor & Francis GroupBioTechniques0736-62051940-98182003-09-0135348849210.2144/03353st01Epitope tagging: a monoclonal antibody specific for recombinant fusion proteins in plantsSusan D. Lawrence0Nicole G. Novak1Jeffrey M. Slack21United States Department of Agriculture, Beltsville, MD, USA1United States Department of Agriculture, Beltsville, MD, USA1United States Department of Agriculture, Beltsville, MD, USAThe easy identification of a recombinant protein in plant material becomes increasingly relevant as more transgenic plants are used for research and commercial applications. Tagging recombinant proteins with a small peptide (epitope) can perform such a task. However, available epitope antibodies will also cross-react with endogenous plant proteins at a level that may be unacceptable. Here we describe the new epitope antibody AcV5. Whether it is attached to the carboxyl terminal end of enhanced green fluorescent protein (EGFP) or the Bacillus thuringiensis endotoxin Cry3A, these proteins remain functional. In addition, using less than 250 pg AcV5-tagged EGFP produces a strong signal on Western blots with no cross-reactivity of proteins from a broad range of plants of agronomic importance.https://www.future-science.com/doi/10.2144/03353st01 |
| spellingShingle | Susan D. Lawrence Nicole G. Novak Jeffrey M. Slack Epitope tagging: a monoclonal antibody specific for recombinant fusion proteins in plants BioTechniques |
| title | Epitope tagging: a monoclonal antibody specific for recombinant fusion proteins in plants |
| title_full | Epitope tagging: a monoclonal antibody specific for recombinant fusion proteins in plants |
| title_fullStr | Epitope tagging: a monoclonal antibody specific for recombinant fusion proteins in plants |
| title_full_unstemmed | Epitope tagging: a monoclonal antibody specific for recombinant fusion proteins in plants |
| title_short | Epitope tagging: a monoclonal antibody specific for recombinant fusion proteins in plants |
| title_sort | epitope tagging a monoclonal antibody specific for recombinant fusion proteins in plants |
| url | https://www.future-science.com/doi/10.2144/03353st01 |
| work_keys_str_mv | AT susandlawrence epitopetaggingamonoclonalantibodyspecificforrecombinantfusionproteinsinplants AT nicolegnovak epitopetaggingamonoclonalantibodyspecificforrecombinantfusionproteinsinplants AT jeffreymslack epitopetaggingamonoclonalantibodyspecificforrecombinantfusionproteinsinplants |