Curcumin-Activated Mesenchymal Stem Cells Derived from Human Umbilical Cord and Their Effects on MPTP-Mouse Model of Parkinson’s Disease: A New Biological Therapy for Parkinson’s Disease
Background. The aim of this study was to investigate the effects of human umbilical cord mesenchymal stem cell activated by curcumin (hUC-MSCs-CUR) on Parkinson’s disease (PD). hUC-MSCs can differentiate into many types of adult tissue cells including dopaminergic (DA) neurons. CUR could protect DA...
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| Format: | Article |
| Language: | English |
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Wiley
2020-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2020/4636397 |
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| author | Yun-Liang Wang Xin-Shan Liu Shan-Shan Wang Peng Xue Zhi-Lei Zeng Xiao-Peng Yang Si-Miao Zhang Wei Zheng Linlin Hua Jin-Feng Li Hai-Tao Wang Shang Guo |
| author_facet | Yun-Liang Wang Xin-Shan Liu Shan-Shan Wang Peng Xue Zhi-Lei Zeng Xiao-Peng Yang Si-Miao Zhang Wei Zheng Linlin Hua Jin-Feng Li Hai-Tao Wang Shang Guo |
| author_sort | Yun-Liang Wang |
| collection | DOAJ |
| description | Background. The aim of this study was to investigate the effects of human umbilical cord mesenchymal stem cell activated by curcumin (hUC-MSCs-CUR) on Parkinson’s disease (PD). hUC-MSCs can differentiate into many types of adult tissue cells including dopaminergic (DA) neurons. CUR could protect DA neurons from apoptosis induced by 6-hydroxydopamine (6-OHDA). Therefore, we used the hUC-MSCs activated by CUR for the treatment of PD in an animal model. Methods. The hUC-MSCs-CUR was transplanted into the MPTP-induced PD mouse models via the tail vein. We found that hUC-MSCs-CUR significantly improved the motor ability, increased the tyrosine hydroxylase (TH), dopamine (DA), and Bcl-2 levels, and reduced nitric oxide synthase, Bax, and cleaved caspase 3 expression in PD mice. The supernatant of hUC-MSCs-CUR (CM-CUR) was used to stimulate the SH-SY5Y cellular model of PD; cell proliferation, differentiation, TH, and neuronal-specific marker microtubular-associated protein 2 (MAP2) expressions were examined. Results. Our data showed that CM-CUR significantly promoted cell proliferation and gradually increased TH and MAP2 expression in SH-SY5Y PD cells. The beneficial effects could be associated with significant increase of rough endoplasmic reticulum in the hUC-MSCs-CUR, which secretes many cytokines and growth factors beneficial for PD treatment. Conclusions. Transplantation of hUC-MSCs-CUR could show promise for improving the motor recovery of PD. |
| format | Article |
| id | doaj-art-ecf0b324ca794f6f83ea010715aff5e2 |
| institution | OA Journals |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-ecf0b324ca794f6f83ea010715aff5e22025-08-20T02:22:09ZengWileyStem Cells International1687-966X1687-96782020-01-01202010.1155/2020/46363974636397Curcumin-Activated Mesenchymal Stem Cells Derived from Human Umbilical Cord and Their Effects on MPTP-Mouse Model of Parkinson’s Disease: A New Biological Therapy for Parkinson’s DiseaseYun-Liang Wang0Xin-Shan Liu1Shan-Shan Wang2Peng Xue3Zhi-Lei Zeng4Xiao-Peng Yang5Si-Miao Zhang6Wei Zheng7Linlin Hua8Jin-Feng Li9Hai-Tao Wang10Shang Guo11Department of Neurology, The Second Affiliated Hospital of Zhengzhou University, 2 Jingba Road, Zhengzhou 450014, ChinaSanbo Brain Hospital, Capital Medical University, 50 Xiang Shan Yikesong Road, Beijing 100093, ChinaDepartment of Neurology, The 960th Hospital, 20 Zhanbei Road, Zibo 255300, ChinaDepartment of Neurology, The Second Affiliated Hospital of Zhengzhou University, 2 Jingba Road, Zhengzhou 450014, ChinaDepartment of Neurology, The Second Affiliated Hospital of Zhengzhou University, 2 Jingba Road, Zhengzhou 450014, ChinaDepartment of Neurology, The Second Affiliated Hospital of Zhengzhou University, 2 Jingba Road, Zhengzhou 450014, ChinaDepartment of Neurology, The Second Affiliated Hospital of Zhengzhou University, 2 Jingba Road, Zhengzhou 450014, ChinaDepartment of Neurology, The Second Affiliated Hospital of Zhengzhou University, 2 Jingba Road, Zhengzhou 450014, ChinaDepartment of Neurology, The Second Affiliated Hospital of Zhengzhou University, 2 Jingba Road, Zhengzhou 450014, ChinaDepartment of Oncology, Chinese PLA General Hospital, Beijing 100037, ChinaDepartment of Cardiology, The 970th Hospital, No. 7 Zhichunan Road, Shandong 264002, ChinaShanghai Jiao Tong University Affiliated Sixth People’s Hospital, Shanghai 200233, ChinaBackground. The aim of this study was to investigate the effects of human umbilical cord mesenchymal stem cell activated by curcumin (hUC-MSCs-CUR) on Parkinson’s disease (PD). hUC-MSCs can differentiate into many types of adult tissue cells including dopaminergic (DA) neurons. CUR could protect DA neurons from apoptosis induced by 6-hydroxydopamine (6-OHDA). Therefore, we used the hUC-MSCs activated by CUR for the treatment of PD in an animal model. Methods. The hUC-MSCs-CUR was transplanted into the MPTP-induced PD mouse models via the tail vein. We found that hUC-MSCs-CUR significantly improved the motor ability, increased the tyrosine hydroxylase (TH), dopamine (DA), and Bcl-2 levels, and reduced nitric oxide synthase, Bax, and cleaved caspase 3 expression in PD mice. The supernatant of hUC-MSCs-CUR (CM-CUR) was used to stimulate the SH-SY5Y cellular model of PD; cell proliferation, differentiation, TH, and neuronal-specific marker microtubular-associated protein 2 (MAP2) expressions were examined. Results. Our data showed that CM-CUR significantly promoted cell proliferation and gradually increased TH and MAP2 expression in SH-SY5Y PD cells. The beneficial effects could be associated with significant increase of rough endoplasmic reticulum in the hUC-MSCs-CUR, which secretes many cytokines and growth factors beneficial for PD treatment. Conclusions. Transplantation of hUC-MSCs-CUR could show promise for improving the motor recovery of PD.http://dx.doi.org/10.1155/2020/4636397 |
| spellingShingle | Yun-Liang Wang Xin-Shan Liu Shan-Shan Wang Peng Xue Zhi-Lei Zeng Xiao-Peng Yang Si-Miao Zhang Wei Zheng Linlin Hua Jin-Feng Li Hai-Tao Wang Shang Guo Curcumin-Activated Mesenchymal Stem Cells Derived from Human Umbilical Cord and Their Effects on MPTP-Mouse Model of Parkinson’s Disease: A New Biological Therapy for Parkinson’s Disease Stem Cells International |
| title | Curcumin-Activated Mesenchymal Stem Cells Derived from Human Umbilical Cord and Their Effects on MPTP-Mouse Model of Parkinson’s Disease: A New Biological Therapy for Parkinson’s Disease |
| title_full | Curcumin-Activated Mesenchymal Stem Cells Derived from Human Umbilical Cord and Their Effects on MPTP-Mouse Model of Parkinson’s Disease: A New Biological Therapy for Parkinson’s Disease |
| title_fullStr | Curcumin-Activated Mesenchymal Stem Cells Derived from Human Umbilical Cord and Their Effects on MPTP-Mouse Model of Parkinson’s Disease: A New Biological Therapy for Parkinson’s Disease |
| title_full_unstemmed | Curcumin-Activated Mesenchymal Stem Cells Derived from Human Umbilical Cord and Their Effects on MPTP-Mouse Model of Parkinson’s Disease: A New Biological Therapy for Parkinson’s Disease |
| title_short | Curcumin-Activated Mesenchymal Stem Cells Derived from Human Umbilical Cord and Their Effects on MPTP-Mouse Model of Parkinson’s Disease: A New Biological Therapy for Parkinson’s Disease |
| title_sort | curcumin activated mesenchymal stem cells derived from human umbilical cord and their effects on mptp mouse model of parkinson s disease a new biological therapy for parkinson s disease |
| url | http://dx.doi.org/10.1155/2020/4636397 |
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