Divergent B-cell repertoire remodelling by mRNA, DNA and live attenuated vaccines in fish
Abstract Vaccination is critical for the future of aquaculture, and nucleic acid vaccines have a major potential for fighting emerging fish infectious diseases, yet their mechanisms remain poorly understood. We compared B-cell responses induced by an mRNA, a DNA, and an attenuated vaccine, all encod...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2025-07-01
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| Series: | npj Vaccines |
| Online Access: | https://doi.org/10.1038/s41541-025-01232-8 |
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| author | Dean Porter Catherine Collins Andrea Mazzolini Luc Jouneau Vanessa Mhanna Céline Coiffier Mathilde Peruzzi Yan Jaszczyszyn Encarnita Mariotti-Ferrandiz Francois Huetz Bertrand Collet Thierry Mora Aleksandra M. Walczak Bernard Verrier Pierre Boudinot |
| author_facet | Dean Porter Catherine Collins Andrea Mazzolini Luc Jouneau Vanessa Mhanna Céline Coiffier Mathilde Peruzzi Yan Jaszczyszyn Encarnita Mariotti-Ferrandiz Francois Huetz Bertrand Collet Thierry Mora Aleksandra M. Walczak Bernard Verrier Pierre Boudinot |
| author_sort | Dean Porter |
| collection | DOAJ |
| description | Abstract Vaccination is critical for the future of aquaculture, and nucleic acid vaccines have a major potential for fighting emerging fish infectious diseases, yet their mechanisms remain poorly understood. We compared B-cell responses induced by an mRNA, a DNA, and an attenuated vaccine, all encoding the same antigen against a fish rhabdovirus. Rainbow trout IgHμ repertoires were examined to investigate how vaccines reshape clonal composition and complexity of the B-cell repertoire. The attenuated virus drove protection through a small number of highly shared public clonotypes encoding neutralizing antibodies. The mRNA vaccine profoundly remodelled the repertoire in some individuals and induces low, but still protective, neutralising Ab titers without public expansions. The DNA vaccine induced high neutralizing Ab titers, providing full protection with minimal impact on B-cell repertoire. Clustering analysis revealed partial sharing of private responses between fish. These findings highlight profound divergences between fish B-cell responses to nucleic acid and attenuated vaccines whilst all of three vaccines induce protective responses. |
| format | Article |
| id | doaj-art-ece3493df4fd4f608959dcbbbe4896ed |
| institution | Kabale University |
| issn | 2059-0105 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | npj Vaccines |
| spelling | doaj-art-ece3493df4fd4f608959dcbbbe4896ed2025-08-20T03:45:40ZengNature Portfolionpj Vaccines2059-01052025-07-0110111610.1038/s41541-025-01232-8Divergent B-cell repertoire remodelling by mRNA, DNA and live attenuated vaccines in fishDean Porter0Catherine Collins1Andrea Mazzolini2Luc Jouneau3Vanessa Mhanna4Céline Coiffier5Mathilde Peruzzi6Yan Jaszczyszyn7Encarnita Mariotti-Ferrandiz8Francois Huetz9Bertrand Collet10Thierry Mora11Aleksandra M. Walczak12Bernard Verrier13Pierre Boudinot14Université Paris-Saclay, INRAE, UVSQ, VIMUniversité Paris-Saclay, INRAE, UVSQ, VIMLaboratoire de physique de l’école normale supérieure (PSL University), CNRS, Sorbonne Université and Université Paris CitéUniversité Paris-Saclay, INRAE, UVSQ, VIMSorbonne Université, INSERM, Immunology-Immunopathology-Immunotherapy (i3)UMR 5305: Laboratoire de Biologie Tissulaire et d’Ingénierie ThérapeutiqueUniversité Paris-Saclay, INRAE, UVSQ, VIMUniversité Paris-Saclay, CEA, CNRS, Institute for Integrative Biology of the Cell (I2BC)Sorbonne Université, INSERM, Immunology-Immunopathology-Immunotherapy (i3)Institut Pasteur, Université Paris Cité, INSERM UMR1222, Antibodies in Therapy and PathologyUniversité Paris-Saclay, INRAE, UVSQ, VIMLaboratoire de physique de l’école normale supérieure (PSL University), CNRS, Sorbonne Université and Université Paris CitéLaboratoire de physique de l’école normale supérieure (PSL University), CNRS, Sorbonne Université and Université Paris CitéUMR 5305: Laboratoire de Biologie Tissulaire et d’Ingénierie ThérapeutiqueUniversité Paris-Saclay, INRAE, UVSQ, VIMAbstract Vaccination is critical for the future of aquaculture, and nucleic acid vaccines have a major potential for fighting emerging fish infectious diseases, yet their mechanisms remain poorly understood. We compared B-cell responses induced by an mRNA, a DNA, and an attenuated vaccine, all encoding the same antigen against a fish rhabdovirus. Rainbow trout IgHμ repertoires were examined to investigate how vaccines reshape clonal composition and complexity of the B-cell repertoire. The attenuated virus drove protection through a small number of highly shared public clonotypes encoding neutralizing antibodies. The mRNA vaccine profoundly remodelled the repertoire in some individuals and induces low, but still protective, neutralising Ab titers without public expansions. The DNA vaccine induced high neutralizing Ab titers, providing full protection with minimal impact on B-cell repertoire. Clustering analysis revealed partial sharing of private responses between fish. These findings highlight profound divergences between fish B-cell responses to nucleic acid and attenuated vaccines whilst all of three vaccines induce protective responses.https://doi.org/10.1038/s41541-025-01232-8 |
| spellingShingle | Dean Porter Catherine Collins Andrea Mazzolini Luc Jouneau Vanessa Mhanna Céline Coiffier Mathilde Peruzzi Yan Jaszczyszyn Encarnita Mariotti-Ferrandiz Francois Huetz Bertrand Collet Thierry Mora Aleksandra M. Walczak Bernard Verrier Pierre Boudinot Divergent B-cell repertoire remodelling by mRNA, DNA and live attenuated vaccines in fish npj Vaccines |
| title | Divergent B-cell repertoire remodelling by mRNA, DNA and live attenuated vaccines in fish |
| title_full | Divergent B-cell repertoire remodelling by mRNA, DNA and live attenuated vaccines in fish |
| title_fullStr | Divergent B-cell repertoire remodelling by mRNA, DNA and live attenuated vaccines in fish |
| title_full_unstemmed | Divergent B-cell repertoire remodelling by mRNA, DNA and live attenuated vaccines in fish |
| title_short | Divergent B-cell repertoire remodelling by mRNA, DNA and live attenuated vaccines in fish |
| title_sort | divergent b cell repertoire remodelling by mrna dna and live attenuated vaccines in fish |
| url | https://doi.org/10.1038/s41541-025-01232-8 |
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