Abdominal Fat and Sarcopenia in Women Significantly Alter Osteoblasts Homeostasis In Vitro by a WNT/β-Catenin Dependent Mechanism
Obesity and sarcopenia have been associated with mineral metabolism derangement and low bone mineral density (BMD). We investigated whether imbalance of serum factors in obese or obese sarcopenic patients could affect bone cell activity in vitro. To evaluate and characterize potential cellular and m...
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| Language: | English |
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Wiley
2014-01-01
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| Series: | International Journal of Endocrinology |
| Online Access: | http://dx.doi.org/10.1155/2014/278316 |
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| author | Francesca Wannenes Vincenza Papa Emanuela A. Greco Rachele Fornari Chiara Marocco Carlo Baldari Luigi Di Luigi Gian Pietro Emerenziani Eleonora Poggiogalle Laura Guidetti Lorenzo M. Donini Andrea Lenzi Silvia Migliaccio |
| author_facet | Francesca Wannenes Vincenza Papa Emanuela A. Greco Rachele Fornari Chiara Marocco Carlo Baldari Luigi Di Luigi Gian Pietro Emerenziani Eleonora Poggiogalle Laura Guidetti Lorenzo M. Donini Andrea Lenzi Silvia Migliaccio |
| author_sort | Francesca Wannenes |
| collection | DOAJ |
| description | Obesity and sarcopenia have been associated with mineral metabolism derangement and low bone mineral density (BMD). We investigated whether imbalance of serum factors in obese or obese sarcopenic patients could affect bone cell activity in vitro. To evaluate and characterize potential cellular and molecular changes of human osteoblasts, cells were exposed to sera of four groups of patients: (1) affected by obesity with normal BMD (O), (2) affected by obesity with low BMD (OO), (3) affected by obesity and sarcopenia (OS), and (4) affected by obesity, sarcopenia, and low BMD (OOS) as compared to subjects with normal body weight and normal BMD (CTL). Patients were previously investigated and characterized for body composition, biochemical and bone turnover markers. Then, sera of different groups of patients were used to incubate human osteoblasts and evaluate potential alterations in cell homeostasis. Exposure to OO, OS, and OOS sera significantly reduced alkaline phosphatase, osteopontin, and BMP4 expression compared to cells exposed to O and CTL, indicating a detrimental effect on osteoblast differentiation. Interestingly, sera of all groups of patients induced intracellular alteration in Wnt/β-catenin molecular pathway, as demonstrated by the significant alteration of specific target genes expression and by altered β-catenin cellular compartmentalization and GSK3β phosphorylation. In conclusion our results show for the first time that sera of obese subjects with low bone mineral density and sarcopenia significantly alter osteoblasts homeostasis in vitro, indicating potential detrimental effects of trunk fat on bone formation and skeletal homeostasis. |
| format | Article |
| id | doaj-art-ecdecac2bd484c5e90c265ab538d66c1 |
| institution | OA Journals |
| issn | 1687-8337 1687-8345 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Endocrinology |
| spelling | doaj-art-ecdecac2bd484c5e90c265ab538d66c12025-08-20T02:22:06ZengWileyInternational Journal of Endocrinology1687-83371687-83452014-01-01201410.1155/2014/278316278316Abdominal Fat and Sarcopenia in Women Significantly Alter Osteoblasts Homeostasis In Vitro by a WNT/β-Catenin Dependent MechanismFrancesca Wannenes0Vincenza Papa1Emanuela A. Greco2Rachele Fornari3Chiara Marocco4Carlo Baldari5Luigi Di Luigi6Gian Pietro Emerenziani7Eleonora Poggiogalle8Laura Guidetti9Lorenzo M. Donini10Andrea Lenzi11Silvia Migliaccio12Section of Health Sciences, Department of Movement, Human and Health Sciences, “Foro Italico” University of Rome, Largo Lauro De Bosis 15, 00195 Rome, ItalySection of Health Sciences, Department of Movement, Human and Health Sciences, “Foro Italico” University of Rome, Largo Lauro De Bosis 15, 00195 Rome, ItalySection of Medical Pathophysiology, Department of Experimental Medicine, Endocrinology and Nutrition, “Sapienza” University of Rome, ItalySection of Medical Pathophysiology, Department of Experimental Medicine, Endocrinology and Nutrition, “Sapienza” University of Rome, ItalySection of Medical Pathophysiology, Department of Experimental Medicine, Endocrinology and Nutrition, “Sapienza” University of Rome, ItalySection of Health Sciences, Department of Movement, Human and Health Sciences, “Foro Italico” University of Rome, Largo Lauro De Bosis 15, 00195 Rome, ItalySection of Health Sciences, Department of Movement, Human and Health Sciences, “Foro Italico” University of Rome, Largo Lauro De Bosis 15, 00195 Rome, ItalySection of Health Sciences, Department of Movement, Human and Health Sciences, “Foro Italico” University of Rome, Largo Lauro De Bosis 15, 00195 Rome, ItalySection of Medical Pathophysiology, Department of Experimental Medicine, Endocrinology and Nutrition, “Sapienza” University of Rome, ItalySection of Health Sciences, Department of Movement, Human and Health Sciences, “Foro Italico” University of Rome, Largo Lauro De Bosis 15, 00195 Rome, ItalySection of Medical Pathophysiology, Department of Experimental Medicine, Endocrinology and Nutrition, “Sapienza” University of Rome, ItalySection of Medical Pathophysiology, Department of Experimental Medicine, Endocrinology and Nutrition, “Sapienza” University of Rome, ItalySection of Health Sciences, Department of Movement, Human and Health Sciences, “Foro Italico” University of Rome, Largo Lauro De Bosis 15, 00195 Rome, ItalyObesity and sarcopenia have been associated with mineral metabolism derangement and low bone mineral density (BMD). We investigated whether imbalance of serum factors in obese or obese sarcopenic patients could affect bone cell activity in vitro. To evaluate and characterize potential cellular and molecular changes of human osteoblasts, cells were exposed to sera of four groups of patients: (1) affected by obesity with normal BMD (O), (2) affected by obesity with low BMD (OO), (3) affected by obesity and sarcopenia (OS), and (4) affected by obesity, sarcopenia, and low BMD (OOS) as compared to subjects with normal body weight and normal BMD (CTL). Patients were previously investigated and characterized for body composition, biochemical and bone turnover markers. Then, sera of different groups of patients were used to incubate human osteoblasts and evaluate potential alterations in cell homeostasis. Exposure to OO, OS, and OOS sera significantly reduced alkaline phosphatase, osteopontin, and BMP4 expression compared to cells exposed to O and CTL, indicating a detrimental effect on osteoblast differentiation. Interestingly, sera of all groups of patients induced intracellular alteration in Wnt/β-catenin molecular pathway, as demonstrated by the significant alteration of specific target genes expression and by altered β-catenin cellular compartmentalization and GSK3β phosphorylation. In conclusion our results show for the first time that sera of obese subjects with low bone mineral density and sarcopenia significantly alter osteoblasts homeostasis in vitro, indicating potential detrimental effects of trunk fat on bone formation and skeletal homeostasis.http://dx.doi.org/10.1155/2014/278316 |
| spellingShingle | Francesca Wannenes Vincenza Papa Emanuela A. Greco Rachele Fornari Chiara Marocco Carlo Baldari Luigi Di Luigi Gian Pietro Emerenziani Eleonora Poggiogalle Laura Guidetti Lorenzo M. Donini Andrea Lenzi Silvia Migliaccio Abdominal Fat and Sarcopenia in Women Significantly Alter Osteoblasts Homeostasis In Vitro by a WNT/β-Catenin Dependent Mechanism International Journal of Endocrinology |
| title | Abdominal Fat and Sarcopenia in Women Significantly Alter Osteoblasts Homeostasis In Vitro by a WNT/β-Catenin Dependent Mechanism |
| title_full | Abdominal Fat and Sarcopenia in Women Significantly Alter Osteoblasts Homeostasis In Vitro by a WNT/β-Catenin Dependent Mechanism |
| title_fullStr | Abdominal Fat and Sarcopenia in Women Significantly Alter Osteoblasts Homeostasis In Vitro by a WNT/β-Catenin Dependent Mechanism |
| title_full_unstemmed | Abdominal Fat and Sarcopenia in Women Significantly Alter Osteoblasts Homeostasis In Vitro by a WNT/β-Catenin Dependent Mechanism |
| title_short | Abdominal Fat and Sarcopenia in Women Significantly Alter Osteoblasts Homeostasis In Vitro by a WNT/β-Catenin Dependent Mechanism |
| title_sort | abdominal fat and sarcopenia in women significantly alter osteoblasts homeostasis in vitro by a wnt β catenin dependent mechanism |
| url | http://dx.doi.org/10.1155/2014/278316 |
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