Emergence of blaKPC-2-carrying megaplasmid in carbapenem-resistant Pseudomonas aeruginosa ST1076: Genomic and functional insights
Objective: Carbapenem-resistant Pseudomonas aeruginosa (CRPA) poses a critical challenge to clinical treatment due to its multidrug resistance and genomic plasticity. Here, we report the genomic and functional characterization of an ST1076 P. aeruginosa isolate carrying blaKPC-2 on a transferable pl...
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Elsevier
2025-09-01
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| Series: | Journal of Global Antimicrobial Resistance |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213716525001766 |
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| author | Jing Chen Weidong Zhu Chen Lin Zhen Li Haowei Ye Danfeng Lou |
| author_facet | Jing Chen Weidong Zhu Chen Lin Zhen Li Haowei Ye Danfeng Lou |
| author_sort | Jing Chen |
| collection | DOAJ |
| description | Objective: Carbapenem-resistant Pseudomonas aeruginosa (CRPA) poses a critical challenge to clinical treatment due to its multidrug resistance and genomic plasticity. Here, we report the genomic and functional characterization of an ST1076 P. aeruginosa isolate carrying blaKPC-2 on a transferable plasmid. Methods: Species identification was performed by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS), and blaKPC-2 was identified by polymerase chain reaction. The characteristics of P. aeruginosa L4884 were analysed by whole-genome sequencing and antimicrobial susceptibility testing. Plasmid characterization was carried out through S1-nuclease pulsed-field gel electrophoresis (S1-PFGE), Southern blotting, transconjugation experiments, and comparative genomic analysis. Results: The isolate, P. aeruginosa L4884, belonged to sequence type ST1076 and demonstrated resistance to multiple β-lactam antibiotics, including imipenem and meropenem. The blaKPC-2 gene was located on a 394-kb IncP-2 megaplasmid, displaying a conserved genetic structure (IS6100-ISKpn27-blaKPC-2-ISKpn6-klcA). Transconjugation experiments succeeded after repeated efforts, confirming the plasmid’s ability to transfer at 27°C. Conclusions: This study provides new insights into the genetic basis of carbapenem resistance and underscores the need for enhanced surveillance and containment strategies to prevent the spread of CRPA in clinical settings. |
| format | Article |
| id | doaj-art-ecdd2e53dd984e46af9e1398f49eb3c0 |
| institution | Kabale University |
| issn | 2213-7165 |
| language | English |
| publishDate | 2025-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Global Antimicrobial Resistance |
| spelling | doaj-art-ecdd2e53dd984e46af9e1398f49eb3c02025-08-20T03:46:50ZengElsevierJournal of Global Antimicrobial Resistance2213-71652025-09-014434735210.1016/j.jgar.2025.07.018Emergence of blaKPC-2-carrying megaplasmid in carbapenem-resistant Pseudomonas aeruginosa ST1076: Genomic and functional insightsJing Chen0Weidong Zhu1Chen Lin2Zhen Li3Haowei Ye4Danfeng Lou5Department of Nursing, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, ChinaDepartment of Emergency Medicine, Traditional Chinese Medical Hospital of Zhuji, Zhuji, ChinaDepartment of Nursing, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, ChinaDepartment of Pathology, The Affiliated Changsha Hospital of Xiangya School of Medicine, Central South University/The First Hospital of Changsha, Changsha, ChinaState Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, ChinaDepartment of Infectious Diseases, Shulan (Hangzhou) Hospital, Shulan International Medical College, Zhejiang Shuren University, Hangzhou, China; Corresponding author. Mailing address: Department of Infectious Diseases, Shulan (Hangzhou) Hospital, No. 848 Dongxin Road, Hangzhou 310006, China.Objective: Carbapenem-resistant Pseudomonas aeruginosa (CRPA) poses a critical challenge to clinical treatment due to its multidrug resistance and genomic plasticity. Here, we report the genomic and functional characterization of an ST1076 P. aeruginosa isolate carrying blaKPC-2 on a transferable plasmid. Methods: Species identification was performed by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS), and blaKPC-2 was identified by polymerase chain reaction. The characteristics of P. aeruginosa L4884 were analysed by whole-genome sequencing and antimicrobial susceptibility testing. Plasmid characterization was carried out through S1-nuclease pulsed-field gel electrophoresis (S1-PFGE), Southern blotting, transconjugation experiments, and comparative genomic analysis. Results: The isolate, P. aeruginosa L4884, belonged to sequence type ST1076 and demonstrated resistance to multiple β-lactam antibiotics, including imipenem and meropenem. The blaKPC-2 gene was located on a 394-kb IncP-2 megaplasmid, displaying a conserved genetic structure (IS6100-ISKpn27-blaKPC-2-ISKpn6-klcA). Transconjugation experiments succeeded after repeated efforts, confirming the plasmid’s ability to transfer at 27°C. Conclusions: This study provides new insights into the genetic basis of carbapenem resistance and underscores the need for enhanced surveillance and containment strategies to prevent the spread of CRPA in clinical settings.http://www.sciencedirect.com/science/article/pii/S2213716525001766Pseudomonas aeruginosaST1076blaKPC-2IncP-2 megaplasmidClonal dissemination |
| spellingShingle | Jing Chen Weidong Zhu Chen Lin Zhen Li Haowei Ye Danfeng Lou Emergence of blaKPC-2-carrying megaplasmid in carbapenem-resistant Pseudomonas aeruginosa ST1076: Genomic and functional insights Journal of Global Antimicrobial Resistance Pseudomonas aeruginosa ST1076 blaKPC-2 IncP-2 megaplasmid Clonal dissemination |
| title | Emergence of blaKPC-2-carrying megaplasmid in carbapenem-resistant Pseudomonas aeruginosa ST1076: Genomic and functional insights |
| title_full | Emergence of blaKPC-2-carrying megaplasmid in carbapenem-resistant Pseudomonas aeruginosa ST1076: Genomic and functional insights |
| title_fullStr | Emergence of blaKPC-2-carrying megaplasmid in carbapenem-resistant Pseudomonas aeruginosa ST1076: Genomic and functional insights |
| title_full_unstemmed | Emergence of blaKPC-2-carrying megaplasmid in carbapenem-resistant Pseudomonas aeruginosa ST1076: Genomic and functional insights |
| title_short | Emergence of blaKPC-2-carrying megaplasmid in carbapenem-resistant Pseudomonas aeruginosa ST1076: Genomic and functional insights |
| title_sort | emergence of blakpc 2 carrying megaplasmid in carbapenem resistant pseudomonas aeruginosa st1076 genomic and functional insights |
| topic | Pseudomonas aeruginosa ST1076 blaKPC-2 IncP-2 megaplasmid Clonal dissemination |
| url | http://www.sciencedirect.com/science/article/pii/S2213716525001766 |
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