Synthesis and Characterization of a Novel Chitosan-Based Nanoparticle–Hydrogel Composite System Promising for Skin Wound Drug Delivery

As a natural preservative, nisin is widely used in the food industry, while its application in biomedicine is limited due to its susceptibility to interference from external conditions. In this study, a nanoparticle–hydrogel composite system was designed to encapsulate and release nisin. Nisin nanop...

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Main Authors: Yueying Huang, Shuting Hao, Jiayu Chen, Mengyuan Wang, Ziheng Lin, Yanan Liu
Format: Article
Language:English
Published: MDPI AG 2024-09-01
Series:Marine Drugs
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Online Access:https://www.mdpi.com/1660-3397/22/9/428
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author Yueying Huang
Shuting Hao
Jiayu Chen
Mengyuan Wang
Ziheng Lin
Yanan Liu
author_facet Yueying Huang
Shuting Hao
Jiayu Chen
Mengyuan Wang
Ziheng Lin
Yanan Liu
author_sort Yueying Huang
collection DOAJ
description As a natural preservative, nisin is widely used in the food industry, while its application in biomedicine is limited due to its susceptibility to interference from external conditions. In this study, a nanoparticle–hydrogel composite system was designed to encapsulate and release nisin. Nisin nanoparticles were identified with a smooth, spherical visual morphology, particle size of 122.72 ± 4.88 nm, polydispersity coefficient of 0.473 ± 0.063, and zeta potential of 23.89 ± 0.37 mV. Based on the sample state and critical properties, three temperature-sensitive hydrogels based on chitosan were ultimately chosen with a rapid gelation time of 112 s, outstanding reticular structure, and optimal swelling ratio of 239.05 ± 7.15%. The composite system exhibited the same antibacterial properties as nisin, demonstrated by the composite system’s inhibition zone diameter of 17.06 ± 0.83 mm, compared to 20.20 ± 0.58 mm for nisin, which was attributed to the prolonged release effect of the hydrogel at the appropriate temperature. The composite system also demonstrated good biocompatibility and safety, making it suitable for application as short-term wound dressings in biomedicine due to its low hemolysis rate of less than 2%. In summary, our nanoparticle-based hydrogel composite system offers a novel application form of nisin while ensuring its stability, thereby deepening and broadening the employment of nisin.
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issn 1660-3397
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publishDate 2024-09-01
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series Marine Drugs
spelling doaj-art-ecd2b85ecd64481ea8d809cb0b40a67b2025-08-20T01:55:38ZengMDPI AGMarine Drugs1660-33972024-09-0122942810.3390/md22090428Synthesis and Characterization of a Novel Chitosan-Based Nanoparticle–Hydrogel Composite System Promising for Skin Wound Drug DeliveryYueying Huang0Shuting Hao1Jiayu Chen2Mengyuan Wang3Ziheng Lin4Yanan Liu5Department of Food Science and Engineering, Ningbo University, Ningbo 315800, ChinaDepartment of Food Science and Engineering, Ningbo University, Ningbo 315800, ChinaDepartment of Food Science and Engineering, Ningbo University, Ningbo 315800, ChinaDepartment of Food Science and Engineering, Ningbo University, Ningbo 315800, ChinaDepartment of Food Science and Engineering, Ningbo University, Ningbo 315800, ChinaDepartment of Food Science and Engineering, Ningbo University, Ningbo 315800, ChinaAs a natural preservative, nisin is widely used in the food industry, while its application in biomedicine is limited due to its susceptibility to interference from external conditions. In this study, a nanoparticle–hydrogel composite system was designed to encapsulate and release nisin. Nisin nanoparticles were identified with a smooth, spherical visual morphology, particle size of 122.72 ± 4.88 nm, polydispersity coefficient of 0.473 ± 0.063, and zeta potential of 23.89 ± 0.37 mV. Based on the sample state and critical properties, three temperature-sensitive hydrogels based on chitosan were ultimately chosen with a rapid gelation time of 112 s, outstanding reticular structure, and optimal swelling ratio of 239.05 ± 7.15%. The composite system exhibited the same antibacterial properties as nisin, demonstrated by the composite system’s inhibition zone diameter of 17.06 ± 0.83 mm, compared to 20.20 ± 0.58 mm for nisin, which was attributed to the prolonged release effect of the hydrogel at the appropriate temperature. The composite system also demonstrated good biocompatibility and safety, making it suitable for application as short-term wound dressings in biomedicine due to its low hemolysis rate of less than 2%. In summary, our nanoparticle-based hydrogel composite system offers a novel application form of nisin while ensuring its stability, thereby deepening and broadening the employment of nisin.https://www.mdpi.com/1660-3397/22/9/428nisinnanoparticlesthermo-sensitive hydrogelscomposite drug delivery systembacteriostasis
spellingShingle Yueying Huang
Shuting Hao
Jiayu Chen
Mengyuan Wang
Ziheng Lin
Yanan Liu
Synthesis and Characterization of a Novel Chitosan-Based Nanoparticle–Hydrogel Composite System Promising for Skin Wound Drug Delivery
Marine Drugs
nisin
nanoparticles
thermo-sensitive hydrogels
composite drug delivery system
bacteriostasis
title Synthesis and Characterization of a Novel Chitosan-Based Nanoparticle–Hydrogel Composite System Promising for Skin Wound Drug Delivery
title_full Synthesis and Characterization of a Novel Chitosan-Based Nanoparticle–Hydrogel Composite System Promising for Skin Wound Drug Delivery
title_fullStr Synthesis and Characterization of a Novel Chitosan-Based Nanoparticle–Hydrogel Composite System Promising for Skin Wound Drug Delivery
title_full_unstemmed Synthesis and Characterization of a Novel Chitosan-Based Nanoparticle–Hydrogel Composite System Promising for Skin Wound Drug Delivery
title_short Synthesis and Characterization of a Novel Chitosan-Based Nanoparticle–Hydrogel Composite System Promising for Skin Wound Drug Delivery
title_sort synthesis and characterization of a novel chitosan based nanoparticle hydrogel composite system promising for skin wound drug delivery
topic nisin
nanoparticles
thermo-sensitive hydrogels
composite drug delivery system
bacteriostasis
url https://www.mdpi.com/1660-3397/22/9/428
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