Isolation and Functional Determination of SKOR Potassium Channel in Purple Osier Willow, Salix purpurea
Potassium (K+) plays key roles in plant growth and development. However, molecular mechanism studies of K+ nutrition in forest plants are largely rare. In plants, SKOR gene encodes for the outward rectifying Shaker-type K+ channel that is responsible for the long-distance transportation of K+ throug...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2021-01-01
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| Series: | International Journal of Genomics |
| Online Access: | http://dx.doi.org/10.1155/2021/6669509 |
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| author | Yahui Chen Xuefeng Peng Jijie Cui Hongxia Zhang Jiang Jiang Zhizhong Song |
| author_facet | Yahui Chen Xuefeng Peng Jijie Cui Hongxia Zhang Jiang Jiang Zhizhong Song |
| author_sort | Yahui Chen |
| collection | DOAJ |
| description | Potassium (K+) plays key roles in plant growth and development. However, molecular mechanism studies of K+ nutrition in forest plants are largely rare. In plants, SKOR gene encodes for the outward rectifying Shaker-type K+ channel that is responsible for the long-distance transportation of K+ through xylem in roots. In this study, we determined a Shaker-type K+ channel gene in purple osier (Salix purpurea), designated as SpuSKOR, and determined its function using a patch clamp electrophysiological system. SpuSKOR was closely clustered with poplar PtrSKOR in the phylogenetic tree. Quantitative real-time PCR (qRT-PCR) analyses demonstrated that SpuSKOR was predominantly expressed in roots, and expression decreased under K+ depletion conditions. Patch clamp analysis via HEK293-T cells demonstrated that the activity of the SpuSKOR channel was activated when the cell membrane voltage reached at -10 mV, and the channel activity was enhanced along with the increase of membrane voltage. Outward currents were recorded and induced in response to the decrease of external K+ concentration. Our results indicate that SpuSKOR is a typical voltage dependent outwardly rectifying K+ channel in purple osier. This study provides theoretical basis for revealing the mechanism of K+ transport and distribution in woody plants. |
| format | Article |
| id | doaj-art-ecd09c65fbf446718d9dfbbf4f65b250 |
| institution | OA Journals |
| issn | 2314-436X 2314-4378 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Genomics |
| spelling | doaj-art-ecd09c65fbf446718d9dfbbf4f65b2502025-08-20T02:22:06ZengWileyInternational Journal of Genomics2314-436X2314-43782021-01-01202110.1155/2021/66695096669509Isolation and Functional Determination of SKOR Potassium Channel in Purple Osier Willow, Salix purpureaYahui Chen0Xuefeng Peng1Jijie Cui2Hongxia Zhang3Jiang Jiang4Zhizhong Song5Collaborative Innovation Center of Sustainable Forestry in Southern China of Jiangsu Province, Nanjing Forestry University, Nanjing, Jiangsu, ChinaThe Engineering Research Institute of Agriculture and Forestry, Ludong University, Yantai, Shandong, ChinaThe Engineering Research Institute of Agriculture and Forestry, Ludong University, Yantai, Shandong, ChinaThe Engineering Research Institute of Agriculture and Forestry, Ludong University, Yantai, Shandong, ChinaCollaborative Innovation Center of Sustainable Forestry in Southern China of Jiangsu Province, Nanjing Forestry University, Nanjing, Jiangsu, ChinaCollaborative Innovation Center of Sustainable Forestry in Southern China of Jiangsu Province, Nanjing Forestry University, Nanjing, Jiangsu, ChinaPotassium (K+) plays key roles in plant growth and development. However, molecular mechanism studies of K+ nutrition in forest plants are largely rare. In plants, SKOR gene encodes for the outward rectifying Shaker-type K+ channel that is responsible for the long-distance transportation of K+ through xylem in roots. In this study, we determined a Shaker-type K+ channel gene in purple osier (Salix purpurea), designated as SpuSKOR, and determined its function using a patch clamp electrophysiological system. SpuSKOR was closely clustered with poplar PtrSKOR in the phylogenetic tree. Quantitative real-time PCR (qRT-PCR) analyses demonstrated that SpuSKOR was predominantly expressed in roots, and expression decreased under K+ depletion conditions. Patch clamp analysis via HEK293-T cells demonstrated that the activity of the SpuSKOR channel was activated when the cell membrane voltage reached at -10 mV, and the channel activity was enhanced along with the increase of membrane voltage. Outward currents were recorded and induced in response to the decrease of external K+ concentration. Our results indicate that SpuSKOR is a typical voltage dependent outwardly rectifying K+ channel in purple osier. This study provides theoretical basis for revealing the mechanism of K+ transport and distribution in woody plants.http://dx.doi.org/10.1155/2021/6669509 |
| spellingShingle | Yahui Chen Xuefeng Peng Jijie Cui Hongxia Zhang Jiang Jiang Zhizhong Song Isolation and Functional Determination of SKOR Potassium Channel in Purple Osier Willow, Salix purpurea International Journal of Genomics |
| title | Isolation and Functional Determination of SKOR Potassium Channel in Purple Osier Willow, Salix purpurea |
| title_full | Isolation and Functional Determination of SKOR Potassium Channel in Purple Osier Willow, Salix purpurea |
| title_fullStr | Isolation and Functional Determination of SKOR Potassium Channel in Purple Osier Willow, Salix purpurea |
| title_full_unstemmed | Isolation and Functional Determination of SKOR Potassium Channel in Purple Osier Willow, Salix purpurea |
| title_short | Isolation and Functional Determination of SKOR Potassium Channel in Purple Osier Willow, Salix purpurea |
| title_sort | isolation and functional determination of skor potassium channel in purple osier willow salix purpurea |
| url | http://dx.doi.org/10.1155/2021/6669509 |
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