Analysis of cytogenetics for the aneuploids in pentaploid progenies from intergeneric cross between Brassica napus and Orychophragmus violaceus

Cytological observation on progenies of intergeneric pentaploids between Brassica napus and Orychophragmus violaceus was conducted and the results indicated that frequency of cells with 47, 44 and 41 chromosomes in somatic cells or pollen mother cells (PMCs) was higher than that with other chromosom...

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Main Authors: WU Jian-guo, SHI Chun-hai, LI Zai-yun, FU Ting-dong
Format: Article
Language:English
Published: Zhejiang University Press 2002-11-01
Series:浙江大学学报. 农业与生命科学版
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Online Access:https://www.academax.com/doi/10.3785/1008-9209.2002.06.0601
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author WU Jian-guo
SHI Chun-hai
LI Zai-yun
FU Ting-dong
author_facet WU Jian-guo
SHI Chun-hai
LI Zai-yun
FU Ting-dong
author_sort WU Jian-guo
collection DOAJ
description Cytological observation on progenies of intergeneric pentaploids between Brassica napus and Orychophragmus violaceus was conducted and the results indicated that frequency of cells with 47, 44 and 41 chromosomes in somatic cells or pollen mother cells (PMCs) was higher than that with other chromosome number in aneuploids among the aneuploid progenies. Except for the plants with 38 chromosomes, they were mixoploid with extra chromosomes derived most likely from O. violaceus. Chromosome elimination, due to exclusion from telophase nuclei and sebsequent digestion of the extra chromosomes, occurred in all PMCs (2n>38). Some modes of segregation that were apt to cause symmetrical distribution of chromosome in PMCs at the metaphase Ⅰ (MI) stage. Moreover, several special segregation modes were discovered in PMCs which led to aggregation of 18 or 17 chromosomes at this stage. In each of three parts (anaphase Ⅰ (AI) stage of PMCs, female gametes and male gametes), chromosome number was lowered in plants with 44 or 41 chromosomes according to gamete constitutes and frequency. Among these three parts, the ratio of male gametes with decreased chromosome number was larger than both of others. Gametes with lower chromosome numbers (n=22-19) were more easily transferred to offsprings. Somatic chromosome diminution was observed in root tips of seedling according to their somatic constituent.
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spelling doaj-art-eccecd7ff02944edb4a2e6286e7c60522025-08-20T02:54:50ZengZhejiang University Press浙江大学学报. 农业与生命科学版1008-92092097-51552002-11-012860160810.3785/1008-9209.2002.06.060110089209Analysis of cytogenetics for the aneuploids in pentaploid progenies from intergeneric cross between Brassica napus and Orychophragmus violaceusWU Jian-guoSHI Chun-haiLI Zai-yunFU Ting-dongCytological observation on progenies of intergeneric pentaploids between Brassica napus and Orychophragmus violaceus was conducted and the results indicated that frequency of cells with 47, 44 and 41 chromosomes in somatic cells or pollen mother cells (PMCs) was higher than that with other chromosome number in aneuploids among the aneuploid progenies. Except for the plants with 38 chromosomes, they were mixoploid with extra chromosomes derived most likely from O. violaceus. Chromosome elimination, due to exclusion from telophase nuclei and sebsequent digestion of the extra chromosomes, occurred in all PMCs (2n&gt;38). Some modes of segregation that were apt to cause symmetrical distribution of chromosome in PMCs at the metaphase Ⅰ (MI) stage. Moreover, several special segregation modes were discovered in PMCs which led to aggregation of 18 or 17 chromosomes at this stage. In each of three parts (anaphase Ⅰ (AI) stage of PMCs, female gametes and male gametes), chromosome number was lowered in plants with 44 or 41 chromosomes according to gamete constitutes and frequency. Among these three parts, the ratio of male gametes with decreased chromosome number was larger than both of others. Gametes with lower chromosome numbers (n=22-19) were more easily transferred to offsprings. Somatic chromosome diminution was observed in root tips of seedling according to their somatic constituent.https://www.academax.com/doi/10.3785/1008-9209.2002.06.0601<italic>Brassica napus</italic><italic>Orychophragmus violaceus</italic>cytogeneticschromosome eliminationaneuploid
spellingShingle WU Jian-guo
SHI Chun-hai
LI Zai-yun
FU Ting-dong
Analysis of cytogenetics for the aneuploids in pentaploid progenies from intergeneric cross between Brassica napus and Orychophragmus violaceus
浙江大学学报. 农业与生命科学版
<italic>Brassica napus</italic>
<italic>Orychophragmus violaceus</italic>
cytogenetics
chromosome elimination
aneuploid
title Analysis of cytogenetics for the aneuploids in pentaploid progenies from intergeneric cross between Brassica napus and Orychophragmus violaceus
title_full Analysis of cytogenetics for the aneuploids in pentaploid progenies from intergeneric cross between Brassica napus and Orychophragmus violaceus
title_fullStr Analysis of cytogenetics for the aneuploids in pentaploid progenies from intergeneric cross between Brassica napus and Orychophragmus violaceus
title_full_unstemmed Analysis of cytogenetics for the aneuploids in pentaploid progenies from intergeneric cross between Brassica napus and Orychophragmus violaceus
title_short Analysis of cytogenetics for the aneuploids in pentaploid progenies from intergeneric cross between Brassica napus and Orychophragmus violaceus
title_sort analysis of cytogenetics for the aneuploids in pentaploid progenies from intergeneric cross between brassica napus and orychophragmus violaceus
topic <italic>Brassica napus</italic>
<italic>Orychophragmus violaceus</italic>
cytogenetics
chromosome elimination
aneuploid
url https://www.academax.com/doi/10.3785/1008-9209.2002.06.0601
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