Effect of Alginate Lyase on Biofilm-Grown Helicobacter pylori Probed by Atomic Force Microscopy
Helicobacter pylori (H. pylori) is a microorganism with a pronounced capability of adaptation under environmental stress solicitations. Its persistence and antimicrobial resistance to the drugs commonly used in the anti-H. pylori therapy are associated with the development of a biofilm mainly compos...
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| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | International Journal of Polymer Science |
| Online Access: | http://dx.doi.org/10.1155/2015/989516 |
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| author | Alessandro Maiorana Francesca Bugli Massimiliano Papi Riccardo Torelli Gabriele Ciasca Giuseppe Maulucci Valentina Palmieri Margherita Cacaci Francesco Paroni Sterbini Brunella Posteraro Maurizio Sanguinetti Marco De Spirito |
| author_facet | Alessandro Maiorana Francesca Bugli Massimiliano Papi Riccardo Torelli Gabriele Ciasca Giuseppe Maulucci Valentina Palmieri Margherita Cacaci Francesco Paroni Sterbini Brunella Posteraro Maurizio Sanguinetti Marco De Spirito |
| author_sort | Alessandro Maiorana |
| collection | DOAJ |
| description | Helicobacter pylori (H. pylori) is a microorganism with a pronounced capability of adaptation under environmental stress solicitations. Its persistence and antimicrobial resistance to the drugs commonly used in the anti-H. pylori therapy are associated with the development of a biofilm mainly composed of DNA, proteins, and polysaccharides. A fundamental step to increase the success of clinical treatments is the development of new strategies and molecules able to interfere with the biofilm architecture and thus able to enhance the effects of antibiotics. By using Atomic Force Microscopy and Scanning Electron Microscopy we analyzed the effects of the alginate lyase (AlgL), an enzyme able to degrade a wide class of polysaccharides, on the H. pylori shape, surface morphology, and biofilm adhesion properties. We demonstrated that AlgL generates a noticeable loss of H. pylori coccoid form in favor of the bacillary form and reduces the H. pylori extracellular polymeric substances (EPS). |
| format | Article |
| id | doaj-art-eccbb1c8ec764bf9973c05199e5385ce |
| institution | OA Journals |
| issn | 1687-9422 1687-9430 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Polymer Science |
| spelling | doaj-art-eccbb1c8ec764bf9973c05199e5385ce2025-08-20T02:22:06ZengWileyInternational Journal of Polymer Science1687-94221687-94302015-01-01201510.1155/2015/989516989516Effect of Alginate Lyase on Biofilm-Grown Helicobacter pylori Probed by Atomic Force MicroscopyAlessandro Maiorana0Francesca Bugli1Massimiliano Papi2Riccardo Torelli3Gabriele Ciasca4Giuseppe Maulucci5Valentina Palmieri6Margherita Cacaci7Francesco Paroni Sterbini8Brunella Posteraro9Maurizio Sanguinetti10Marco De Spirito11Istituto di Fisica, Università Cattolica del Sacro Cuore, Largo F. Vito 1, 00168 Roma, ItalyIstituto di Microbiologia, Università Cattolica del Sacro Cuore, Largo F. Vito 1, 00168 Roma, ItalyIstituto di Fisica, Università Cattolica del Sacro Cuore, Largo F. Vito 1, 00168 Roma, ItalyIstituto di Microbiologia, Università Cattolica del Sacro Cuore, Largo F. Vito 1, 00168 Roma, ItalyIstituto di Fisica, Università Cattolica del Sacro Cuore, Largo F. Vito 1, 00168 Roma, ItalyIstituto di Fisica, Università Cattolica del Sacro Cuore, Largo F. Vito 1, 00168 Roma, ItalyIstituto di Fisica, Università Cattolica del Sacro Cuore, Largo F. Vito 1, 00168 Roma, ItalyIstituto di Microbiologia, Università Cattolica del Sacro Cuore, Largo F. Vito 1, 00168 Roma, ItalyIstituto di Microbiologia, Università Cattolica del Sacro Cuore, Largo F. Vito 1, 00168 Roma, ItalyIstituto di Microbiologia, Università Cattolica del Sacro Cuore, Largo F. Vito 1, 00168 Roma, ItalyIstituto di Microbiologia, Università Cattolica del Sacro Cuore, Largo F. Vito 1, 00168 Roma, ItalyIstituto di Fisica, Università Cattolica del Sacro Cuore, Largo F. Vito 1, 00168 Roma, ItalyHelicobacter pylori (H. pylori) is a microorganism with a pronounced capability of adaptation under environmental stress solicitations. Its persistence and antimicrobial resistance to the drugs commonly used in the anti-H. pylori therapy are associated with the development of a biofilm mainly composed of DNA, proteins, and polysaccharides. A fundamental step to increase the success of clinical treatments is the development of new strategies and molecules able to interfere with the biofilm architecture and thus able to enhance the effects of antibiotics. By using Atomic Force Microscopy and Scanning Electron Microscopy we analyzed the effects of the alginate lyase (AlgL), an enzyme able to degrade a wide class of polysaccharides, on the H. pylori shape, surface morphology, and biofilm adhesion properties. We demonstrated that AlgL generates a noticeable loss of H. pylori coccoid form in favor of the bacillary form and reduces the H. pylori extracellular polymeric substances (EPS).http://dx.doi.org/10.1155/2015/989516 |
| spellingShingle | Alessandro Maiorana Francesca Bugli Massimiliano Papi Riccardo Torelli Gabriele Ciasca Giuseppe Maulucci Valentina Palmieri Margherita Cacaci Francesco Paroni Sterbini Brunella Posteraro Maurizio Sanguinetti Marco De Spirito Effect of Alginate Lyase on Biofilm-Grown Helicobacter pylori Probed by Atomic Force Microscopy International Journal of Polymer Science |
| title | Effect of Alginate Lyase on Biofilm-Grown Helicobacter pylori Probed by Atomic Force Microscopy |
| title_full | Effect of Alginate Lyase on Biofilm-Grown Helicobacter pylori Probed by Atomic Force Microscopy |
| title_fullStr | Effect of Alginate Lyase on Biofilm-Grown Helicobacter pylori Probed by Atomic Force Microscopy |
| title_full_unstemmed | Effect of Alginate Lyase on Biofilm-Grown Helicobacter pylori Probed by Atomic Force Microscopy |
| title_short | Effect of Alginate Lyase on Biofilm-Grown Helicobacter pylori Probed by Atomic Force Microscopy |
| title_sort | effect of alginate lyase on biofilm grown helicobacter pylori probed by atomic force microscopy |
| url | http://dx.doi.org/10.1155/2015/989516 |
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