Sorrel Extract Reduces Oxidant Production in Airway Epithelial Cells Exposed to Swine Barn Dust Extract In Vitro
Exposure to hog barn organic dust contributes to occupational lung diseases, which are mediated by inflammatory and oxidative stress pathways. Isoprostanes—a family of eicosanoids produced by oxidation of phospholipids by oxygen radicals—are biomarkers of pulmonary oxidative stress. Importantly, 8-i...
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| Format: | Article |
| Language: | English |
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Wiley
2019-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2019/7420468 |
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| author | Carresse L. Gerald Chakia J. McClendon Rohit S. Ranabhat Jenora T. Waterman Lauren L. Kloc Dawn R. Conklin Ke’Yona T. Barton Janak R. Khatiwada Leonard L. Williams |
| author_facet | Carresse L. Gerald Chakia J. McClendon Rohit S. Ranabhat Jenora T. Waterman Lauren L. Kloc Dawn R. Conklin Ke’Yona T. Barton Janak R. Khatiwada Leonard L. Williams |
| author_sort | Carresse L. Gerald |
| collection | DOAJ |
| description | Exposure to hog barn organic dust contributes to occupational lung diseases, which are mediated by inflammatory and oxidative stress pathways. Isoprostanes—a family of eicosanoids produced by oxidation of phospholipids by oxygen radicals—are biomarkers of pulmonary oxidative stress. Importantly, 8-isoprostane has been implicated as a key biomarker and mediator of oxidative stress because it is a potent pulmonary vasoconstrictor. Antioxidants found in fruits and vegetables hold promise for preventing or reducing effects of oxidative stress-related diseases including chronic bronchitis and chronic obstructive pulmonary disease (COPD). Here, we investigated 8-isoP and oxidant production by organic dust-exposed airway epithelial cells and the inhibitory effects of an extract from calyces of the sorrel plant, Hibiscus sabdariffa, on oxidant-producing pathways. Confluent cultures of normal human tracheobronchial epithelial cells were pretreated or not with 1% sorrel extract prior to 5% dust extract (DE) exposure. Following DE treatments, live cells, cell-free supernatants, or cell extracts were evaluated for the presence of 8-isoprostane, superoxide, hydrogen peroxide, nitric oxide, hydroxyl radical, peroxynitrite, and catalase activity to evaluate sorrel’s inhibitory effect on oxidative stress. The well-known radical scavenging antioxidant, N-acetyl cysteine (NAC), was used for comparisons with sorrel. DE exposure augmented the production of all radicals measured including 8-isoprostane (p value < 0.001), which could be inhibited by NAC or sorrel. Among reactive oxygen and nitrogen species generated in response to DE exposure, sorrel had no effect on H2O2 production and NAC had no significant effect on NO⋅ production. The observations reported here suggest a possible role for sorrel in preventing 8-isoprostane and oxidant-mediated stress responses in bronchial epithelial cells exposed to hog barn dust. These findings suggest a potential role for oxidative stress pathways in mediating occupational lung diseases and antioxidants within sorrel and NAC in reducing dust-mediated oxidative stress within the airways of exposed workers. |
| format | Article |
| id | doaj-art-eccb741fc7c947bd9b00312461565cea |
| institution | OA Journals |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-eccb741fc7c947bd9b00312461565cea2025-08-20T02:22:06ZengWileyMediators of Inflammation0962-93511466-18612019-01-01201910.1155/2019/74204687420468Sorrel Extract Reduces Oxidant Production in Airway Epithelial Cells Exposed to Swine Barn Dust Extract In VitroCarresse L. Gerald0Chakia J. McClendon1Rohit S. Ranabhat2Jenora T. Waterman3Lauren L. Kloc4Dawn R. Conklin5Ke’Yona T. Barton6Janak R. Khatiwada7Leonard L. Williams8Department of Animal Sciences, North Carolina Agricultural and Technical State University, 1601 East Market Street, Greensboro, NC, USADepartment of Animal Sciences, North Carolina Agricultural and Technical State University, 1601 East Market Street, Greensboro, NC, USADepartment of Animal Sciences, North Carolina Agricultural and Technical State University, 1601 East Market Street, Greensboro, NC, USADepartment of Animal Sciences, North Carolina Agricultural and Technical State University, 1601 East Market Street, Greensboro, NC, USADepartment of Animal Sciences, North Carolina Agricultural and Technical State University, 1601 East Market Street, Greensboro, NC, USADepartment of Animal Sciences, North Carolina Agricultural and Technical State University, 1601 East Market Street, Greensboro, NC, USADepartment of Animal Sciences, North Carolina Agricultural and Technical State University, 1601 East Market Street, Greensboro, NC, USACenter of Excellence for Post-Harvest Technologies, North Carolina Agricultural and Technical University, 500 Laureate Way, Kannapolis, NC, USACenter of Excellence for Post-Harvest Technologies, North Carolina Agricultural and Technical University, 500 Laureate Way, Kannapolis, NC, USAExposure to hog barn organic dust contributes to occupational lung diseases, which are mediated by inflammatory and oxidative stress pathways. Isoprostanes—a family of eicosanoids produced by oxidation of phospholipids by oxygen radicals—are biomarkers of pulmonary oxidative stress. Importantly, 8-isoprostane has been implicated as a key biomarker and mediator of oxidative stress because it is a potent pulmonary vasoconstrictor. Antioxidants found in fruits and vegetables hold promise for preventing or reducing effects of oxidative stress-related diseases including chronic bronchitis and chronic obstructive pulmonary disease (COPD). Here, we investigated 8-isoP and oxidant production by organic dust-exposed airway epithelial cells and the inhibitory effects of an extract from calyces of the sorrel plant, Hibiscus sabdariffa, on oxidant-producing pathways. Confluent cultures of normal human tracheobronchial epithelial cells were pretreated or not with 1% sorrel extract prior to 5% dust extract (DE) exposure. Following DE treatments, live cells, cell-free supernatants, or cell extracts were evaluated for the presence of 8-isoprostane, superoxide, hydrogen peroxide, nitric oxide, hydroxyl radical, peroxynitrite, and catalase activity to evaluate sorrel’s inhibitory effect on oxidative stress. The well-known radical scavenging antioxidant, N-acetyl cysteine (NAC), was used for comparisons with sorrel. DE exposure augmented the production of all radicals measured including 8-isoprostane (p value < 0.001), which could be inhibited by NAC or sorrel. Among reactive oxygen and nitrogen species generated in response to DE exposure, sorrel had no effect on H2O2 production and NAC had no significant effect on NO⋅ production. The observations reported here suggest a possible role for sorrel in preventing 8-isoprostane and oxidant-mediated stress responses in bronchial epithelial cells exposed to hog barn dust. These findings suggest a potential role for oxidative stress pathways in mediating occupational lung diseases and antioxidants within sorrel and NAC in reducing dust-mediated oxidative stress within the airways of exposed workers.http://dx.doi.org/10.1155/2019/7420468 |
| spellingShingle | Carresse L. Gerald Chakia J. McClendon Rohit S. Ranabhat Jenora T. Waterman Lauren L. Kloc Dawn R. Conklin Ke’Yona T. Barton Janak R. Khatiwada Leonard L. Williams Sorrel Extract Reduces Oxidant Production in Airway Epithelial Cells Exposed to Swine Barn Dust Extract In Vitro Mediators of Inflammation |
| title | Sorrel Extract Reduces Oxidant Production in Airway Epithelial Cells Exposed to Swine Barn Dust Extract In Vitro |
| title_full | Sorrel Extract Reduces Oxidant Production in Airway Epithelial Cells Exposed to Swine Barn Dust Extract In Vitro |
| title_fullStr | Sorrel Extract Reduces Oxidant Production in Airway Epithelial Cells Exposed to Swine Barn Dust Extract In Vitro |
| title_full_unstemmed | Sorrel Extract Reduces Oxidant Production in Airway Epithelial Cells Exposed to Swine Barn Dust Extract In Vitro |
| title_short | Sorrel Extract Reduces Oxidant Production in Airway Epithelial Cells Exposed to Swine Barn Dust Extract In Vitro |
| title_sort | sorrel extract reduces oxidant production in airway epithelial cells exposed to swine barn dust extract in vitro |
| url | http://dx.doi.org/10.1155/2019/7420468 |
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