Establishment of Cre/LoxP-mediated multifunctional reporter knock-in rats with the CRISPR system.
Rats and mice are essential experimental animals in preclinical research, serving as models for various human diseases and contributing significantly to drug development. Rats offer distinct advantages over mice due to their larger size, which allows for more complex surgical procedures, repeated bl...
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| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2025-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0325444 |
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| _version_ | 1849429914603749376 |
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| author | Katsuyuki Nakamura Sara Ito Yoshihiro Ohguchi Toshie Jimbo Yusaku Wada Ryota Nakajima Masanobu Kanou Kei Yamana Hiroshi Ueda |
| author_facet | Katsuyuki Nakamura Sara Ito Yoshihiro Ohguchi Toshie Jimbo Yusaku Wada Ryota Nakajima Masanobu Kanou Kei Yamana Hiroshi Ueda |
| author_sort | Katsuyuki Nakamura |
| collection | DOAJ |
| description | Rats and mice are essential experimental animals in preclinical research, serving as models for various human diseases and contributing significantly to drug development. Rats offer distinct advantages over mice due to their larger size, which allows for more complex surgical procedures, repeated blood sampling, or sophisticated behavioral analysis. However, unlike the case with mice, genetically modified rat lines for achieving complex experimental objectives-such as tissue-specific gene knockout or visualization of specific cell populations-are still limited. We here established LoxP-mediated multifunctional reporter KI rats, enabling us to evaluate fluorescence, bioluminescence, and cell-killing assays simultaneously with only one gene-modified rat line. CRISPR/Cas12a, also known as CRISPR/Cpf1, was successfully used to insert the Cre sequence into a target locus to generate Cre driver rats. These results will contribute to the application of gene-modified rats for a more comprehensive understanding of physiology, and for extrapolation of their capabilities in preclinical research. |
| format | Article |
| id | doaj-art-ecca823e498449f19d54b7eba9932241 |
| institution | Kabale University |
| issn | 1932-6203 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-ecca823e498449f19d54b7eba99322412025-08-20T03:28:10ZengPublic Library of Science (PLoS)PLoS ONE1932-62032025-01-01206e032544410.1371/journal.pone.0325444Establishment of Cre/LoxP-mediated multifunctional reporter knock-in rats with the CRISPR system.Katsuyuki NakamuraSara ItoYoshihiro OhguchiToshie JimboYusaku WadaRyota NakajimaMasanobu KanouKei YamanaHiroshi UedaRats and mice are essential experimental animals in preclinical research, serving as models for various human diseases and contributing significantly to drug development. Rats offer distinct advantages over mice due to their larger size, which allows for more complex surgical procedures, repeated blood sampling, or sophisticated behavioral analysis. However, unlike the case with mice, genetically modified rat lines for achieving complex experimental objectives-such as tissue-specific gene knockout or visualization of specific cell populations-are still limited. We here established LoxP-mediated multifunctional reporter KI rats, enabling us to evaluate fluorescence, bioluminescence, and cell-killing assays simultaneously with only one gene-modified rat line. CRISPR/Cas12a, also known as CRISPR/Cpf1, was successfully used to insert the Cre sequence into a target locus to generate Cre driver rats. These results will contribute to the application of gene-modified rats for a more comprehensive understanding of physiology, and for extrapolation of their capabilities in preclinical research.https://doi.org/10.1371/journal.pone.0325444 |
| spellingShingle | Katsuyuki Nakamura Sara Ito Yoshihiro Ohguchi Toshie Jimbo Yusaku Wada Ryota Nakajima Masanobu Kanou Kei Yamana Hiroshi Ueda Establishment of Cre/LoxP-mediated multifunctional reporter knock-in rats with the CRISPR system. PLoS ONE |
| title | Establishment of Cre/LoxP-mediated multifunctional reporter knock-in rats with the CRISPR system. |
| title_full | Establishment of Cre/LoxP-mediated multifunctional reporter knock-in rats with the CRISPR system. |
| title_fullStr | Establishment of Cre/LoxP-mediated multifunctional reporter knock-in rats with the CRISPR system. |
| title_full_unstemmed | Establishment of Cre/LoxP-mediated multifunctional reporter knock-in rats with the CRISPR system. |
| title_short | Establishment of Cre/LoxP-mediated multifunctional reporter knock-in rats with the CRISPR system. |
| title_sort | establishment of cre loxp mediated multifunctional reporter knock in rats with the crispr system |
| url | https://doi.org/10.1371/journal.pone.0325444 |
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