Development and characterization of microsatellite markers for the Cape gooseberry Physalis peruviana.
Physalis peruviana, commonly known as Cape gooseberry, is an Andean Solanaceae fruit with high nutritional value and interesting medicinal properties. In the present study we report the development and characterization of microsatellite loci from a P. peruviana commercial Colombian genotype. We iden...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2011-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0026719&type=printable |
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| author | Jaime Simbaqueba Pilar Sánchez Erika Sanchez Victor Manuel Núñez Zarantes Maria Isabel Chacon Luz Stella Barrero Leonardo Mariño-Ramírez |
| author_facet | Jaime Simbaqueba Pilar Sánchez Erika Sanchez Victor Manuel Núñez Zarantes Maria Isabel Chacon Luz Stella Barrero Leonardo Mariño-Ramírez |
| author_sort | Jaime Simbaqueba |
| collection | DOAJ |
| description | Physalis peruviana, commonly known as Cape gooseberry, is an Andean Solanaceae fruit with high nutritional value and interesting medicinal properties. In the present study we report the development and characterization of microsatellite loci from a P. peruviana commercial Colombian genotype. We identified 932 imperfect and 201 perfect Simple Sequence Repeats (SSR) loci in untranslated regions (UTRs) and 304 imperfect and 83 perfect SSR loci in coding regions from the assembled Physalis peruviana leaf transcriptome. The UTR SSR loci were used for the development of 162 primers for amplification. The efficiency of these primers was tested via PCR in a panel of seven P. peruviana accessions including Colombia, Kenya and Ecuador ecotypes and one closely related species Physalis floridana. We obtained an amplification rate of 83% and a polymorphic rate of 22%. Here we report the first P. peruviana specific microsatellite set, a valuable tool for a wide variety of applications, including functional diversity, conservation and improvement of the species. |
| format | Article |
| id | doaj-art-ecc80090a73e43518e0d820cd60f5d41 |
| institution | DOAJ |
| issn | 1932-6203 |
| language | English |
| publishDate | 2011-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-ecc80090a73e43518e0d820cd60f5d412025-08-20T03:09:47ZengPublic Library of Science (PLoS)PLoS ONE1932-62032011-01-01610e2671910.1371/journal.pone.0026719Development and characterization of microsatellite markers for the Cape gooseberry Physalis peruviana.Jaime SimbaquebaPilar SánchezErika SanchezVictor Manuel Núñez ZarantesMaria Isabel ChaconLuz Stella BarreroLeonardo Mariño-RamírezPhysalis peruviana, commonly known as Cape gooseberry, is an Andean Solanaceae fruit with high nutritional value and interesting medicinal properties. In the present study we report the development and characterization of microsatellite loci from a P. peruviana commercial Colombian genotype. We identified 932 imperfect and 201 perfect Simple Sequence Repeats (SSR) loci in untranslated regions (UTRs) and 304 imperfect and 83 perfect SSR loci in coding regions from the assembled Physalis peruviana leaf transcriptome. The UTR SSR loci were used for the development of 162 primers for amplification. The efficiency of these primers was tested via PCR in a panel of seven P. peruviana accessions including Colombia, Kenya and Ecuador ecotypes and one closely related species Physalis floridana. We obtained an amplification rate of 83% and a polymorphic rate of 22%. Here we report the first P. peruviana specific microsatellite set, a valuable tool for a wide variety of applications, including functional diversity, conservation and improvement of the species.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0026719&type=printable |
| spellingShingle | Jaime Simbaqueba Pilar Sánchez Erika Sanchez Victor Manuel Núñez Zarantes Maria Isabel Chacon Luz Stella Barrero Leonardo Mariño-Ramírez Development and characterization of microsatellite markers for the Cape gooseberry Physalis peruviana. PLoS ONE |
| title | Development and characterization of microsatellite markers for the Cape gooseberry Physalis peruviana. |
| title_full | Development and characterization of microsatellite markers for the Cape gooseberry Physalis peruviana. |
| title_fullStr | Development and characterization of microsatellite markers for the Cape gooseberry Physalis peruviana. |
| title_full_unstemmed | Development and characterization of microsatellite markers for the Cape gooseberry Physalis peruviana. |
| title_short | Development and characterization of microsatellite markers for the Cape gooseberry Physalis peruviana. |
| title_sort | development and characterization of microsatellite markers for the cape gooseberry physalis peruviana |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0026719&type=printable |
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