Small RNA Landscape of Platelet Dust: Platelet-Derived Extracellular Vesicles from Patients with Non-Small-Cell Lung Cancer
<b>Background:</b> Platelet-derived Extracellular Vesicles, or “Platelet Dust” (PD), are reported as the most-abundant extracellular vesicles in plasma. However, the PD molecular content, especially the small RNA profile, is still poorly characterized. This study aims to characterize PD...
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MDPI AG
2025-05-01
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| Series: | Non-Coding RNA |
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| Online Access: | https://www.mdpi.com/2311-553X/11/3/38 |
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| author | Mafalda Antunes-Ferreira Ilias Glogovitis Diogo Fortunato Silvia D’Ambrosi Mariona Colom Saborit Galina Yahubyan Vesselin Baev Michael Hackenberg Natasa Zarovni Thomas Wurdinger Danijela Koppers-Lalic |
| author_facet | Mafalda Antunes-Ferreira Ilias Glogovitis Diogo Fortunato Silvia D’Ambrosi Mariona Colom Saborit Galina Yahubyan Vesselin Baev Michael Hackenberg Natasa Zarovni Thomas Wurdinger Danijela Koppers-Lalic |
| author_sort | Mafalda Antunes-Ferreira |
| collection | DOAJ |
| description | <b>Background:</b> Platelet-derived Extracellular Vesicles, or “Platelet Dust” (PD), are reported as the most-abundant extracellular vesicles in plasma. However, the PD molecular content, especially the small RNA profile, is still poorly characterized. This study aims to characterize PD and other extracellular vesicles (EVs) in patients with non-small-cell lung cancer (NSCLC), focusing on their small RNA signatures and diagnostic potential. <b>Methods:</b> The EVs were isolated directly from the plasma of healthy donors and patients with NSCLC using the surface markers CD9, CD63, CD81 (overall EVs), and CD61 (PD). Small RNA sequencing was then performed to comprehensively profile the miRNAs. <b>Results:</b> Our analysis revealed distinct small RNA profiles in the EVs and the PD from the patients with NSCLC. The EVs (CD9-, CD63-, and CD81-positive) showed the enrichment of four miRNAs and the depletion of ten miRNAs, while the PD (CD61-positive) exhibited a more complex profile, with nineteen miRNAs enriched and nine miRNAs depleted in the patients with NSCLC compared to those of the healthy controls. <b>Conclusions:</b> This exploratory study enhances our understanding of miRNA composition within different plasma vesicle populations, shedding light on the biology of plasma vesicles and their contents. Furthermore, utilizing an extracellular vesicle isolation method with potential clinical applicability offers the prospect of improved cancer characterization and detection by selecting the most informative subpopulation of plasma vesicles. |
| format | Article |
| id | doaj-art-ecc7642dd2ad4239992c08ad2eeba091 |
| institution | Kabale University |
| issn | 2311-553X |
| language | English |
| publishDate | 2025-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Non-Coding RNA |
| spelling | doaj-art-ecc7642dd2ad4239992c08ad2eeba0912025-08-20T03:27:36ZengMDPI AGNon-Coding RNA2311-553X2025-05-011133810.3390/ncrna11030038Small RNA Landscape of Platelet Dust: Platelet-Derived Extracellular Vesicles from Patients with Non-Small-Cell Lung CancerMafalda Antunes-Ferreira0Ilias Glogovitis1Diogo Fortunato2Silvia D’Ambrosi3Mariona Colom Saborit4Galina Yahubyan5Vesselin Baev6Michael Hackenberg7Natasa Zarovni8Thomas Wurdinger9Danijela Koppers-Lalic10Cancer Center Amsterdam, Amsterdam University Medical Centers, 1081 HV Amsterdam, The NetherlandsCancer Center Amsterdam, Amsterdam University Medical Centers, 1081 HV Amsterdam, The NetherlandsCancer Center Amsterdam, Amsterdam University Medical Centers, 1081 HV Amsterdam, The NetherlandsCancer Center Amsterdam, Amsterdam University Medical Centers, 1081 HV Amsterdam, The NetherlandsCancer Center Amsterdam, Amsterdam University Medical Centers, 1081 HV Amsterdam, The NetherlandsDepartment of Molecular Biology, University of Plovdiv, Tzar Assen 24, 4000 Plovdiv, BulgariaDepartment of Molecular Biology, University of Plovdiv, Tzar Assen 24, 4000 Plovdiv, BulgariaGenetics Department, Faculty of Science, Universidad de Granada, Campus de Fuentenueva s/n, 18071 Granada, SpainExosomics SpA, 53100 Siena, ItalyCancer Center Amsterdam, Amsterdam University Medical Centers, 1081 HV Amsterdam, The NetherlandsCancer Center Amsterdam, Amsterdam University Medical Centers, 1081 HV Amsterdam, The Netherlands<b>Background:</b> Platelet-derived Extracellular Vesicles, or “Platelet Dust” (PD), are reported as the most-abundant extracellular vesicles in plasma. However, the PD molecular content, especially the small RNA profile, is still poorly characterized. This study aims to characterize PD and other extracellular vesicles (EVs) in patients with non-small-cell lung cancer (NSCLC), focusing on their small RNA signatures and diagnostic potential. <b>Methods:</b> The EVs were isolated directly from the plasma of healthy donors and patients with NSCLC using the surface markers CD9, CD63, CD81 (overall EVs), and CD61 (PD). Small RNA sequencing was then performed to comprehensively profile the miRNAs. <b>Results:</b> Our analysis revealed distinct small RNA profiles in the EVs and the PD from the patients with NSCLC. The EVs (CD9-, CD63-, and CD81-positive) showed the enrichment of four miRNAs and the depletion of ten miRNAs, while the PD (CD61-positive) exhibited a more complex profile, with nineteen miRNAs enriched and nine miRNAs depleted in the patients with NSCLC compared to those of the healthy controls. <b>Conclusions:</b> This exploratory study enhances our understanding of miRNA composition within different plasma vesicle populations, shedding light on the biology of plasma vesicles and their contents. Furthermore, utilizing an extracellular vesicle isolation method with potential clinical applicability offers the prospect of improved cancer characterization and detection by selecting the most informative subpopulation of plasma vesicles.https://www.mdpi.com/2311-553X/11/3/38plasma vesiclesplatelet-derived vesiclesplatelet dustextracellular vesicles (EVs)small RNA sequencingnon-small-cell lung cancer (NSCLC) |
| spellingShingle | Mafalda Antunes-Ferreira Ilias Glogovitis Diogo Fortunato Silvia D’Ambrosi Mariona Colom Saborit Galina Yahubyan Vesselin Baev Michael Hackenberg Natasa Zarovni Thomas Wurdinger Danijela Koppers-Lalic Small RNA Landscape of Platelet Dust: Platelet-Derived Extracellular Vesicles from Patients with Non-Small-Cell Lung Cancer Non-Coding RNA plasma vesicles platelet-derived vesicles platelet dust extracellular vesicles (EVs) small RNA sequencing non-small-cell lung cancer (NSCLC) |
| title | Small RNA Landscape of Platelet Dust: Platelet-Derived Extracellular Vesicles from Patients with Non-Small-Cell Lung Cancer |
| title_full | Small RNA Landscape of Platelet Dust: Platelet-Derived Extracellular Vesicles from Patients with Non-Small-Cell Lung Cancer |
| title_fullStr | Small RNA Landscape of Platelet Dust: Platelet-Derived Extracellular Vesicles from Patients with Non-Small-Cell Lung Cancer |
| title_full_unstemmed | Small RNA Landscape of Platelet Dust: Platelet-Derived Extracellular Vesicles from Patients with Non-Small-Cell Lung Cancer |
| title_short | Small RNA Landscape of Platelet Dust: Platelet-Derived Extracellular Vesicles from Patients with Non-Small-Cell Lung Cancer |
| title_sort | small rna landscape of platelet dust platelet derived extracellular vesicles from patients with non small cell lung cancer |
| topic | plasma vesicles platelet-derived vesicles platelet dust extracellular vesicles (EVs) small RNA sequencing non-small-cell lung cancer (NSCLC) |
| url | https://www.mdpi.com/2311-553X/11/3/38 |
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