Computational Interrogation of The Human Norovirus-Host Cell Interactions Facilitated by A-Type Antigen
Norovirus infectivity, which causes norovirus-induced gastroenteritis,depends on the interaction between capsid protein VP1 of the virus and hostcell HGBA receptors that tailor the cell membrane surface. The interactionresults in VP1-HGBA complex formation prior to infection. The details of thisinte...
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Sakarya University
2019-12-01
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| Series: | Sakarya Üniversitesi Fen Bilimleri Enstitüsü Dergisi |
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| Online Access: | https://dergipark.org.tr/tr/download/article-file/793007 |
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| author | Abdulkadir Kocak Muslum Yildiz |
| author_facet | Abdulkadir Kocak Muslum Yildiz |
| author_sort | Abdulkadir Kocak |
| collection | DOAJ |
| description | Norovirus infectivity, which causes norovirus-induced gastroenteritis,depends on the interaction between capsid protein VP1 of the virus and hostcell HGBA receptors that tailor the cell membrane surface. The interactionresults in VP1-HGBA complex formation prior to infection. The details of thisinteraction have been provided by x-ray structures of HGBA-VP1 complexes, butthe dynamic nature of this interaction is not fully uncovered. Therefore, thedynamics that drive the formation of VP1-HGBA complex, which is crucial fordeveloping new therapeutic approaches to find a cure for gastroenteritisdisease, need to be elucidated. Here, we computationally analyzed the wild typeVP1 capsid protein in complex with A-type HGBA antigen to unravel interactionsthat are important for virus to enter inside the host cell during infection. Wehave found that the ligand binding causes a fluctuation in a distant loop whichresides in the interface of capsid building blocks, VP proteins. Thisfluctuation leads an instability in capsid particle that may be an indicationfor virus uncoating mechanism during the cell penetration. |
| format | Article |
| id | doaj-art-ecc37d53e54c4bdb869b96d839155ca0 |
| institution | OA Journals |
| issn | 2147-835X |
| language | English |
| publishDate | 2019-12-01 |
| publisher | Sakarya University |
| record_format | Article |
| series | Sakarya Üniversitesi Fen Bilimleri Enstitüsü Dergisi |
| spelling | doaj-art-ecc37d53e54c4bdb869b96d839155ca02025-08-20T02:31:51ZengSakarya UniversitySakarya Üniversitesi Fen Bilimleri Enstitüsü Dergisi2147-835X2019-12-012361056106510.16984/saufenbilder.52543028Computational Interrogation of The Human Norovirus-Host Cell Interactions Facilitated by A-Type AntigenAbdulkadir Kocakhttps://orcid.org/0000-0001-6891-6929Muslum Yildizhttps://orcid.org/0000-0001-8396-1461Norovirus infectivity, which causes norovirus-induced gastroenteritis,depends on the interaction between capsid protein VP1 of the virus and hostcell HGBA receptors that tailor the cell membrane surface. The interactionresults in VP1-HGBA complex formation prior to infection. The details of thisinteraction have been provided by x-ray structures of HGBA-VP1 complexes, butthe dynamic nature of this interaction is not fully uncovered. Therefore, thedynamics that drive the formation of VP1-HGBA complex, which is crucial fordeveloping new therapeutic approaches to find a cure for gastroenteritisdisease, need to be elucidated. Here, we computationally analyzed the wild typeVP1 capsid protein in complex with A-type HGBA antigen to unravel interactionsthat are important for virus to enter inside the host cell during infection. Wehave found that the ligand binding causes a fluctuation in a distant loop whichresides in the interface of capsid building blocks, VP proteins. Thisfluctuation leads an instability in capsid particle that may be an indicationfor virus uncoating mechanism during the cell penetration.https://dergipark.org.tr/tr/download/article-file/793007norovirustherapeutic antibodyhbga blockagemolecular dynamics |
| spellingShingle | Abdulkadir Kocak Muslum Yildiz Computational Interrogation of The Human Norovirus-Host Cell Interactions Facilitated by A-Type Antigen Sakarya Üniversitesi Fen Bilimleri Enstitüsü Dergisi norovirus therapeutic antibody hbga blockage molecular dynamics |
| title | Computational Interrogation of The Human Norovirus-Host Cell Interactions Facilitated by A-Type Antigen |
| title_full | Computational Interrogation of The Human Norovirus-Host Cell Interactions Facilitated by A-Type Antigen |
| title_fullStr | Computational Interrogation of The Human Norovirus-Host Cell Interactions Facilitated by A-Type Antigen |
| title_full_unstemmed | Computational Interrogation of The Human Norovirus-Host Cell Interactions Facilitated by A-Type Antigen |
| title_short | Computational Interrogation of The Human Norovirus-Host Cell Interactions Facilitated by A-Type Antigen |
| title_sort | computational interrogation of the human norovirus host cell interactions facilitated by a type antigen |
| topic | norovirus therapeutic antibody hbga blockage molecular dynamics |
| url | https://dergipark.org.tr/tr/download/article-file/793007 |
| work_keys_str_mv | AT abdulkadirkocak computationalinterrogationofthehumannorovirushostcellinteractionsfacilitatedbyatypeantigen AT muslumyildiz computationalinterrogationofthehumannorovirushostcellinteractionsfacilitatedbyatypeantigen |