Morphological, Physiological, and Molecular Bases of Salt Tolerance in Crape Myrtle (<i>Lagerstroemia indica</i>)
Crape myrtle (<i>Lagerstroemia indica</i> and its relatives) is an important summer-flowering plant in numerous countries worldwide. However, there are few reports on salt-tolerant (ST) crape myrtle germplasm resources and their morphological and molecular biological foundations for adap...
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2024-12-01
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| author | Chunmei Yu Zuorong Ding Tianyi Yuan Cannan Yu Jin Qin Di Lu Hui Wei Fei Zhong Guoyuan Liu Yanhong Chen Jian Zhang |
| author_facet | Chunmei Yu Zuorong Ding Tianyi Yuan Cannan Yu Jin Qin Di Lu Hui Wei Fei Zhong Guoyuan Liu Yanhong Chen Jian Zhang |
| author_sort | Chunmei Yu |
| collection | DOAJ |
| description | Crape myrtle (<i>Lagerstroemia indica</i> and its relatives) is an important summer-flowering plant in numerous countries worldwide. However, there are few reports on salt-tolerant (ST) crape myrtle germplasm resources and their morphological and molecular biological foundations for adaptation to salinized soil, one of the main abiotic stresses in plants. This study identified the salt tolerance characteristics of 19 crape myrtle varieties and created four salt-tolerant germplasms through hybridization. Morphological anatomy and transcriptome analyses clarified that the xylem of the ST variety possessed a high number of vessels with a small lumen. Transcriptome research has indicated that under salt stress conditions, Ca<sup>2+</sup>, abscisic acid (ABA), and reactive oxygen species (ROS) pathways are involved in salt stress responses. One of the candidate genes <i>LiTIP1;1</i> (encoding a tonoplast intrinsic protein) was overexpressed in Arabidopsis and the resultant overexpression (OE) lines performed better under CK and 100 mmol∙L<sup>−1</sup> NaCl salt stress, but not under 200 mmol∙L<sup>−1</sup> NaCl salt stress. Corresponding to physiological traits, genes encoding tubulin and fasciclin-like arabinogalactan proteins (FLAs), which are related to the plasma membrane localization of the cellulose synthase complex, are maintained at higher levels and are induced more by salt. In summary, this research has revealed the morphological, physiological, and molecular bases of ST crape myrtle to a certain extent and has provided a theoretical basis for further screening and breeding of salt-tolerant crape myrtle varieties. |
| format | Article |
| id | doaj-art-ecc08041c9cb4eb491d7287178bf4466 |
| institution | DOAJ |
| issn | 2077-0472 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
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| series | Agriculture |
| spelling | doaj-art-ecc08041c9cb4eb491d7287178bf44662025-08-20T02:53:22ZengMDPI AGAgriculture2077-04722024-12-011412226710.3390/agriculture14122267Morphological, Physiological, and Molecular Bases of Salt Tolerance in Crape Myrtle (<i>Lagerstroemia indica</i>)Chunmei Yu0Zuorong Ding1Tianyi Yuan2Cannan Yu3Jin Qin4Di Lu5Hui Wei6Fei Zhong7Guoyuan Liu8Yanhong Chen9Jian Zhang10School of Life Science, Nantong University, No. 9 Seyuan Road, Nantong 226019, ChinaSchool of Life Science, Nantong University, No. 9 Seyuan Road, Nantong 226019, ChinaSchool of Life Science, Nantong University, No. 9 Seyuan Road, Nantong 226019, ChinaSchool of Life Science, Nantong University, No. 9 Seyuan Road, Nantong 226019, ChinaSchool of Life Science, Nantong University, No. 9 Seyuan Road, Nantong 226019, ChinaSchool of Life Science, Nantong University, No. 9 Seyuan Road, Nantong 226019, ChinaSchool of Life Science, Nantong University, No. 9 Seyuan Road, Nantong 226019, ChinaSchool of Life Science, Nantong University, No. 9 Seyuan Road, Nantong 226019, ChinaSchool of Life Science, Nantong University, No. 9 Seyuan Road, Nantong 226019, ChinaSchool of Life Science, Nantong University, No. 9 Seyuan Road, Nantong 226019, ChinaSchool of Life Science, Nantong University, No. 9 Seyuan Road, Nantong 226019, ChinaCrape myrtle (<i>Lagerstroemia indica</i> and its relatives) is an important summer-flowering plant in numerous countries worldwide. However, there are few reports on salt-tolerant (ST) crape myrtle germplasm resources and their morphological and molecular biological foundations for adaptation to salinized soil, one of the main abiotic stresses in plants. This study identified the salt tolerance characteristics of 19 crape myrtle varieties and created four salt-tolerant germplasms through hybridization. Morphological anatomy and transcriptome analyses clarified that the xylem of the ST variety possessed a high number of vessels with a small lumen. Transcriptome research has indicated that under salt stress conditions, Ca<sup>2+</sup>, abscisic acid (ABA), and reactive oxygen species (ROS) pathways are involved in salt stress responses. One of the candidate genes <i>LiTIP1;1</i> (encoding a tonoplast intrinsic protein) was overexpressed in Arabidopsis and the resultant overexpression (OE) lines performed better under CK and 100 mmol∙L<sup>−1</sup> NaCl salt stress, but not under 200 mmol∙L<sup>−1</sup> NaCl salt stress. Corresponding to physiological traits, genes encoding tubulin and fasciclin-like arabinogalactan proteins (FLAs), which are related to the plasma membrane localization of the cellulose synthase complex, are maintained at higher levels and are induced more by salt. In summary, this research has revealed the morphological, physiological, and molecular bases of ST crape myrtle to a certain extent and has provided a theoretical basis for further screening and breeding of salt-tolerant crape myrtle varieties.https://www.mdpi.com/2077-0472/14/12/2267crape myrtlesaltxylem vesselscelluloseTIP1 |
| spellingShingle | Chunmei Yu Zuorong Ding Tianyi Yuan Cannan Yu Jin Qin Di Lu Hui Wei Fei Zhong Guoyuan Liu Yanhong Chen Jian Zhang Morphological, Physiological, and Molecular Bases of Salt Tolerance in Crape Myrtle (<i>Lagerstroemia indica</i>) Agriculture crape myrtle salt xylem vessels cellulose TIP1 |
| title | Morphological, Physiological, and Molecular Bases of Salt Tolerance in Crape Myrtle (<i>Lagerstroemia indica</i>) |
| title_full | Morphological, Physiological, and Molecular Bases of Salt Tolerance in Crape Myrtle (<i>Lagerstroemia indica</i>) |
| title_fullStr | Morphological, Physiological, and Molecular Bases of Salt Tolerance in Crape Myrtle (<i>Lagerstroemia indica</i>) |
| title_full_unstemmed | Morphological, Physiological, and Molecular Bases of Salt Tolerance in Crape Myrtle (<i>Lagerstroemia indica</i>) |
| title_short | Morphological, Physiological, and Molecular Bases of Salt Tolerance in Crape Myrtle (<i>Lagerstroemia indica</i>) |
| title_sort | morphological physiological and molecular bases of salt tolerance in crape myrtle i lagerstroemia indica i |
| topic | crape myrtle salt xylem vessels cellulose TIP1 |
| url | https://www.mdpi.com/2077-0472/14/12/2267 |
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