Quercetin inhibits the biological activity of endometrial cancer by regulating autophagy: A network pharmacology analysis and cellular experimental validation
To investigate the effects of quercetin (Que) on the improvement of endometrial cancer (EC) and the relative associated mechanisms. Network pharmacology was used to analyze the correlation between Que. and EC. Ishikawa and HEC-1 A cell lines were used as research models in the present study. The cel...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-02-01
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| Series: | Journal of Functional Foods |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464624006601 |
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| author | Lingchen Yang Hongwei Ma |
| author_facet | Lingchen Yang Hongwei Ma |
| author_sort | Lingchen Yang |
| collection | DOAJ |
| description | To investigate the effects of quercetin (Que) on the improvement of endometrial cancer (EC) and the relative associated mechanisms. Network pharmacology was used to analyze the correlation between Que. and EC. Ishikawa and HEC-1 A cell lines were used as research models in the present study. The cell lines were divided into the following groups: Normal, lentivirus (LV)-negative control (NC), Que., Que. + LV-activating transcription factor 5 (ATF5)-RNA interference (RNAi) and Que. + LV-ATF5-RNAi+mTOR agonist. Cell Counting Kit-8 and flow cytometry assays were used to evaluate cell proliferation and apoptosis; cell invasive and migratory abilities were assessed by Transwell and wound healing assays, respectively; the observation of the cell ultrastructure was performed by transmission electron microscopy; the relative protein expressions were measured by the western blot assay. Depending on results, Que. exhibited antitumor effects by stimulating autophagy via regulating the ATF5/JUN/PI3K/AKT/mTOR pathway in EC. |
| format | Article |
| id | doaj-art-ecad29a3b5ea41e790c7614daeb21040 |
| institution | Kabale University |
| issn | 1756-4646 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj-art-ecad29a3b5ea41e790c7614daeb210402025-02-09T04:59:52ZengElsevierJournal of Functional Foods1756-46462025-02-01125106657Quercetin inhibits the biological activity of endometrial cancer by regulating autophagy: A network pharmacology analysis and cellular experimental validationLingchen Yang0Hongwei Ma1Department of Obstetrics, Xuzhou Maternity and Child Health Care Hospital, No. 46 Heping Road, Yunlong District, Xuzhou City, Jiangsu Province 221009, China; Corresponding author: Department of Obstetrics, Xuzhou Maternity and Child Health Care Hospital, No. 46 Heping Road, Yunlong District, Xuzhou City, Jiangsu Province 221009, China.Department of Intensive Care Medicine, Affiliated Hospital of Nantong University, No. 20 Xisi Road, Chongchuan District, Nantong City, Jiangsu Province, 226001, ChinaTo investigate the effects of quercetin (Que) on the improvement of endometrial cancer (EC) and the relative associated mechanisms. Network pharmacology was used to analyze the correlation between Que. and EC. Ishikawa and HEC-1 A cell lines were used as research models in the present study. The cell lines were divided into the following groups: Normal, lentivirus (LV)-negative control (NC), Que., Que. + LV-activating transcription factor 5 (ATF5)-RNA interference (RNAi) and Que. + LV-ATF5-RNAi+mTOR agonist. Cell Counting Kit-8 and flow cytometry assays were used to evaluate cell proliferation and apoptosis; cell invasive and migratory abilities were assessed by Transwell and wound healing assays, respectively; the observation of the cell ultrastructure was performed by transmission electron microscopy; the relative protein expressions were measured by the western blot assay. Depending on results, Que. exhibited antitumor effects by stimulating autophagy via regulating the ATF5/JUN/PI3K/AKT/mTOR pathway in EC.http://www.sciencedirect.com/science/article/pii/S1756464624006601Que.AutophagyEndometrial cancerATF5 |
| spellingShingle | Lingchen Yang Hongwei Ma Quercetin inhibits the biological activity of endometrial cancer by regulating autophagy: A network pharmacology analysis and cellular experimental validation Journal of Functional Foods Que. Autophagy Endometrial cancer ATF5 |
| title | Quercetin inhibits the biological activity of endometrial cancer by regulating autophagy: A network pharmacology analysis and cellular experimental validation |
| title_full | Quercetin inhibits the biological activity of endometrial cancer by regulating autophagy: A network pharmacology analysis and cellular experimental validation |
| title_fullStr | Quercetin inhibits the biological activity of endometrial cancer by regulating autophagy: A network pharmacology analysis and cellular experimental validation |
| title_full_unstemmed | Quercetin inhibits the biological activity of endometrial cancer by regulating autophagy: A network pharmacology analysis and cellular experimental validation |
| title_short | Quercetin inhibits the biological activity of endometrial cancer by regulating autophagy: A network pharmacology analysis and cellular experimental validation |
| title_sort | quercetin inhibits the biological activity of endometrial cancer by regulating autophagy a network pharmacology analysis and cellular experimental validation |
| topic | Que. Autophagy Endometrial cancer ATF5 |
| url | http://www.sciencedirect.com/science/article/pii/S1756464624006601 |
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