Soluble expression of recombinant cyclophilin A in Escherichia coli
To produce a large amount of soluble cyclophilin A (CypA) protein, the recombinant pRSET -CypA plasmid was transferred into E. coli BL21. Using the 2×YT culture medium, the medium compositions of carbon source, nitrogen source and ampicillin concentration were optimized, and the effects of inoculum...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Zhejiang University Press
2010-07-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
| Subjects: | |
| Online Access: | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2010.04.002 |
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