Application of gas chromatography-combustion-stable isotope mass spectrometry in the identification of exogenous alcohol in whisky

Using 129 whiskies from different regions such as Scotland, Ireland, America and Japan as research samples, the differences in carbon stable isotope ratios (δ<sup>13</sup>C) of isobutanol, n-propanol, amyl alcohol, ethyl acetate, acetaldehyde and ethanol among the volatile flavor compone...

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Bibliographic Details
Main Author: CHEN Bo, LUO Jie, HE Chundian, LIU Bin, YI Bingqing, TU Xiaoke, JIN Baohui
Format: Article
Language:English
Published: Editorial Department of China Brewing 2025-04-01
Series:Zhongguo niangzao
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Online Access:https://manu61.magtech.com.cn/zgnz/fileup/0254-5071/PDF/0254-5071-2025-44-4-285.pdf
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Summary:Using 129 whiskies from different regions such as Scotland, Ireland, America and Japan as research samples, the differences in carbon stable isotope ratios (δ<sup>13</sup>C) of isobutanol, n-propanol, amyl alcohol, ethyl acetate, acetaldehyde and ethanol among the volatile flavor components was detected, and a method for the identification of exogenous alcohol in whiskey was established by gas chromatography-combustion-stable isotope mass spectrometry (GC-C-IRMS). Results showed that the δ<sup>13</sup>C of ethanol in Scottish single malt whisky ranged from -27.84‰ to -25.04‰, the δ<sup>13</sup>C of ethanol in Scottish blended whisky ranged from -28.21‰ to -20.72‰, the δ<sup>13</sup>C of ethanol in whisky containing corn raw material ranged from -26.66‰ to -12.83‰, the δ<sup>13</sup>C of ethanol in whisky without corn raw material ranged from -28.86‰ to -24.04‰. Based on the fitted linear intercept deviation range (2SD) to discriminating adulteration, the δ<sup>13</sup>C of ethanol showed good linear relationship with that of n-propanol, isobutanol and acetaldehyde in whisky. The correlation coefficients (R<sup>2</sup>) was 0.876, 0.832, and 0.868, respectively. By combining the linear intercept deviation range with the δ<sup>13</sup>C distribution range of ethanol, the identification of whisky exogenous alcohol is more sensitive.
ISSN:0254-5071