Identification of Two Novel HLA-A∗0201-Restricted CTL Epitopes Derived from MAGE-A4
MAGE-A antigens belong to cancer/testis (CT) antigens that are expressed in tumors but not in normal tissues except testis and placenta. MAGE-A antigens and their epitope peptides have been used in tumor immunotherapy trials. MAGE-A4 antigen is extensively expressed in various histological types of...
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| Format: | Article |
| Language: | English |
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Wiley
2010-01-01
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| Series: | Clinical and Developmental Immunology |
| Online Access: | http://dx.doi.org/10.1155/2010/567594 |
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| author | Zheng-Cai Jia Bing Ni Ze-Min Huang Yi Tian Jun Tang Jing-Xue Wang Xiao-Lan Fu Yu-Zhang Wu |
| author_facet | Zheng-Cai Jia Bing Ni Ze-Min Huang Yi Tian Jun Tang Jing-Xue Wang Xiao-Lan Fu Yu-Zhang Wu |
| author_sort | Zheng-Cai Jia |
| collection | DOAJ |
| description | MAGE-A antigens belong to cancer/testis (CT) antigens that are expressed in tumors but not in normal tissues except testis and placenta. MAGE-A antigens and their epitope peptides have been used in tumor immunotherapy trials. MAGE-A4 antigen is extensively expressed in various histological types of tumors, so it represents an attractive target for tumor immunotherapy. In this study, we predicted HLA-A∗0201-restricted cytotoxic T lymphocyte (CTL) epitopes of MAGE-A4, followed by peptide/HLA-A∗0201 affinity and complex stability assays. Of selected four peptides (designated P1, P2, P3, and P4), P1 (MAGE-A4286-294, KVLEHVVRV) and P3 (MAGE-A4272-280, FLWGPRALA) could elicit peptide-specific CTLs both in vitro from HLA-A∗0201-positive PBMCs and in HLA-A∗0201/Kb transgenic mice. And the induced CTLs could lyse target cells in an HLA-A∗0201-restricted fashion, demonstrating that the two peptides are HLA-A∗0201-restricted CTL epitopes and could serve as targets for therapeutic antitumoral vaccination. |
| format | Article |
| id | doaj-art-ec8e56d2c24c4287bf5b58ecf9aca2be |
| institution | Kabale University |
| issn | 1740-2522 1740-2530 |
| language | English |
| publishDate | 2010-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Clinical and Developmental Immunology |
| spelling | doaj-art-ec8e56d2c24c4287bf5b58ecf9aca2be2025-02-03T01:30:56ZengWileyClinical and Developmental Immunology1740-25221740-25302010-01-01201010.1155/2010/567594567594Identification of Two Novel HLA-A∗0201-Restricted CTL Epitopes Derived from MAGE-A4Zheng-Cai Jia0Bing Ni1Ze-Min Huang2Yi Tian3Jun Tang4Jing-Xue Wang5Xiao-Lan Fu6Yu-Zhang Wu7Department of Immunology, Third Military Medical University, Chongqing 400038, ChinaDepartment of Immunology, Third Military Medical University, Chongqing 400038, ChinaDepartment of Immunology, Third Military Medical University, Chongqing 400038, ChinaDepartment of Immunology, Third Military Medical University, Chongqing 400038, ChinaDepartment of Dermatology, Southwest Hospital, Third Military Medical University, Chongqing 400038, ChinaDepartment of Immunology, Third Military Medical University, Chongqing 400038, ChinaDepartment of Immunology, Third Military Medical University, Chongqing 400038, ChinaDepartment of Immunology, Third Military Medical University, Chongqing 400038, ChinaMAGE-A antigens belong to cancer/testis (CT) antigens that are expressed in tumors but not in normal tissues except testis and placenta. MAGE-A antigens and their epitope peptides have been used in tumor immunotherapy trials. MAGE-A4 antigen is extensively expressed in various histological types of tumors, so it represents an attractive target for tumor immunotherapy. In this study, we predicted HLA-A∗0201-restricted cytotoxic T lymphocyte (CTL) epitopes of MAGE-A4, followed by peptide/HLA-A∗0201 affinity and complex stability assays. Of selected four peptides (designated P1, P2, P3, and P4), P1 (MAGE-A4286-294, KVLEHVVRV) and P3 (MAGE-A4272-280, FLWGPRALA) could elicit peptide-specific CTLs both in vitro from HLA-A∗0201-positive PBMCs and in HLA-A∗0201/Kb transgenic mice. And the induced CTLs could lyse target cells in an HLA-A∗0201-restricted fashion, demonstrating that the two peptides are HLA-A∗0201-restricted CTL epitopes and could serve as targets for therapeutic antitumoral vaccination.http://dx.doi.org/10.1155/2010/567594 |
| spellingShingle | Zheng-Cai Jia Bing Ni Ze-Min Huang Yi Tian Jun Tang Jing-Xue Wang Xiao-Lan Fu Yu-Zhang Wu Identification of Two Novel HLA-A∗0201-Restricted CTL Epitopes Derived from MAGE-A4 Clinical and Developmental Immunology |
| title | Identification of Two Novel HLA-A∗0201-Restricted CTL Epitopes Derived from MAGE-A4 |
| title_full | Identification of Two Novel HLA-A∗0201-Restricted CTL Epitopes Derived from MAGE-A4 |
| title_fullStr | Identification of Two Novel HLA-A∗0201-Restricted CTL Epitopes Derived from MAGE-A4 |
| title_full_unstemmed | Identification of Two Novel HLA-A∗0201-Restricted CTL Epitopes Derived from MAGE-A4 |
| title_short | Identification of Two Novel HLA-A∗0201-Restricted CTL Epitopes Derived from MAGE-A4 |
| title_sort | identification of two novel hla a∗0201 restricted ctl epitopes derived from mage a4 |
| url | http://dx.doi.org/10.1155/2010/567594 |
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