Prominent T-Cell Responses against the Acetylcholine Receptor ε Subunit in Myasthenia Gravis
The human acetylcholine receptor (AChR) is well characterized as the target antigen in myasthenia gravis (MG). Pathogenic antibody responses against the AChR alpha-chain have been investigated extensively and are of diagnostic and prognostic value. However, less is known on the pathogenetic relevanc...
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| Language: | English |
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Wiley
2019-01-01
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| Series: | Neurology Research International |
| Online Access: | http://dx.doi.org/10.1155/2019/1969068 |
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| author | Oliver Neuhaus Karl-Heinz Wiesmüller Hans-Peter Hartung Heinz Wiendl |
| author_facet | Oliver Neuhaus Karl-Heinz Wiesmüller Hans-Peter Hartung Heinz Wiendl |
| author_sort | Oliver Neuhaus |
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| description | The human acetylcholine receptor (AChR) is well characterized as the target antigen in myasthenia gravis (MG). Pathogenic antibody responses against the AChR alpha-chain have been investigated extensively and are of diagnostic and prognostic value. However, less is known on the pathogenetic relevance of T-cell responses against epitopes of the different AChR chains (alpha, epsilon, gamma). Using an enzyme-linked immunospot (ELISPOT) assay we measured T-cell responses against recombinant fragments and synthetic peptides of the α and the ε subunits of the human AChR in MG patients (n=15) and in healthy donors (HD; n=9). In MG, highest T-cell responses were noted against recombinantly expressed Epsilon 1-221. Among the synthetic peptides Epsilon 201-215 showed the most prominent T-cell response and represented the peptide with the most remarkable difference between MG and HD. Taken together, prominent T-cell responses against the ε subunit of the human AChR indicate an important role in the pathogenesis of MG. |
| format | Article |
| id | doaj-art-ec8e18f7567d4c68bb0e099374afcec1 |
| institution | OA Journals |
| issn | 2090-1852 2090-1860 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Neurology Research International |
| spelling | doaj-art-ec8e18f7567d4c68bb0e099374afcec12025-08-20T02:21:49ZengWileyNeurology Research International2090-18522090-18602019-01-01201910.1155/2019/19690681969068Prominent T-Cell Responses against the Acetylcholine Receptor ε Subunit in Myasthenia GravisOliver Neuhaus0Karl-Heinz Wiesmüller1Hans-Peter Hartung2Heinz Wiendl3Department of Neurology, Medical University of Graz, Graz, AustriaEMC Microcollections GmbH, Tübingen, GermanyDepartment of Neurology, Medical University of Graz, Graz, AustriaDepartment of Neurology, University of Tübingen, Tübingen, GermanyThe human acetylcholine receptor (AChR) is well characterized as the target antigen in myasthenia gravis (MG). Pathogenic antibody responses against the AChR alpha-chain have been investigated extensively and are of diagnostic and prognostic value. However, less is known on the pathogenetic relevance of T-cell responses against epitopes of the different AChR chains (alpha, epsilon, gamma). Using an enzyme-linked immunospot (ELISPOT) assay we measured T-cell responses against recombinant fragments and synthetic peptides of the α and the ε subunits of the human AChR in MG patients (n=15) and in healthy donors (HD; n=9). In MG, highest T-cell responses were noted against recombinantly expressed Epsilon 1-221. Among the synthetic peptides Epsilon 201-215 showed the most prominent T-cell response and represented the peptide with the most remarkable difference between MG and HD. Taken together, prominent T-cell responses against the ε subunit of the human AChR indicate an important role in the pathogenesis of MG.http://dx.doi.org/10.1155/2019/1969068 |
| spellingShingle | Oliver Neuhaus Karl-Heinz Wiesmüller Hans-Peter Hartung Heinz Wiendl Prominent T-Cell Responses against the Acetylcholine Receptor ε Subunit in Myasthenia Gravis Neurology Research International |
| title | Prominent T-Cell Responses against the Acetylcholine Receptor ε Subunit in Myasthenia Gravis |
| title_full | Prominent T-Cell Responses against the Acetylcholine Receptor ε Subunit in Myasthenia Gravis |
| title_fullStr | Prominent T-Cell Responses against the Acetylcholine Receptor ε Subunit in Myasthenia Gravis |
| title_full_unstemmed | Prominent T-Cell Responses against the Acetylcholine Receptor ε Subunit in Myasthenia Gravis |
| title_short | Prominent T-Cell Responses against the Acetylcholine Receptor ε Subunit in Myasthenia Gravis |
| title_sort | prominent t cell responses against the acetylcholine receptor ε subunit in myasthenia gravis |
| url | http://dx.doi.org/10.1155/2019/1969068 |
| work_keys_str_mv | AT oliverneuhaus prominenttcellresponsesagainsttheacetylcholinereceptoresubunitinmyastheniagravis AT karlheinzwiesmuller prominenttcellresponsesagainsttheacetylcholinereceptoresubunitinmyastheniagravis AT hanspeterhartung prominenttcellresponsesagainsttheacetylcholinereceptoresubunitinmyastheniagravis AT heinzwiendl prominenttcellresponsesagainsttheacetylcholinereceptoresubunitinmyastheniagravis |