mecA and PVL genes in methicillin-resistant Staphylococcus aureus from clinical specimens: a cross-sectional hospital based study from Nepal
Background and Objectives: Staphylococcus aureus has increasingly been associated with community and healthcare-associated infections worldwide and contributes to treatment failures due to the emergence of multidrug-resistant (MDR) and methicillin-resistant Staphylococcus aureus (MRSA) strains. We...
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Tehran University of Medical Sciences
2025-02-01
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| Series: | Iranian Journal of Microbiology |
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| Online Access: | https://ijm.tums.ac.ir/index.php/ijm/article/view/5035 |
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| author | Sirjana Adhikari Supriya Sharma Sanjib Adhikari Sanjit Shrestha Dwij Raj Bhatta |
| author_facet | Sirjana Adhikari Supriya Sharma Sanjib Adhikari Sanjit Shrestha Dwij Raj Bhatta |
| author_sort | Sirjana Adhikari |
| collection | DOAJ |
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Background and Objectives: Staphylococcus aureus has increasingly been associated with community and healthcare-associated infections worldwide and contributes to treatment failures due to the emergence of multidrug-resistant (MDR) and methicillin-resistant Staphylococcus aureus (MRSA) strains. We aimed to determine the prevalence and antibiotic susceptibility pattern of MRSA isolated from patients attending a burn center and to detect mecA and PVL genes among MRSA isolates.
Materials and Methods: A cross-sectional hospital based study was conducted on 1950 clinical samples collected from hospital inpatients and outpatients of Kirtipur Hospital, which is a burn specialist hospital in Kathmandu, Nepal. Each sample underwent conventional cultural methods for bacterial isolates identification.
Results: Out of 1950 samples, 452 (23.2%) samples showed bacterial growth, of which 109 isolates (24.1%) were identified as Gram positive and 343 (75.9%) as Gram negative bacteria. Among the Gram positive bacteria, 53 (48.62%) were Staphylococcus aureus. Of the total S. aureus isolates, 40 (75.5%) were MRSA and 48 (90.6%) were MDR. Of the 40 MRSA isolates, 29 (72.5%) carried the mecA gene and 3 (7.5%) harbored PVL gene.
Conclusion: The high prevalence of MRSA in a burn unit underscores the need for more rigorous infection control practices that follow standard protocols to reduce MRSA transmission in both individuals and the hospital environment.
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| format | Article |
| id | doaj-art-ec7bade0d41e420c9416696dc1f938a0 |
| institution | Kabale University |
| issn | 2008-3289 2008-4447 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Microbiology |
| spelling | doaj-art-ec7bade0d41e420c9416696dc1f938a02025-02-09T09:01:27ZengTehran University of Medical SciencesIranian Journal of Microbiology2008-32892008-44472025-02-01171mecA and PVL genes in methicillin-resistant Staphylococcus aureus from clinical specimens: a cross-sectional hospital based study from NepalSirjana Adhikari0Supriya Sharma1Sanjib Adhikari2Sanjit Shrestha3Dwij Raj Bhatta4Central Department of Microbiology, Tribhuvan University, Kirtipur, Kathmandu, NepalCentral Department of Microbiology, Tribhuvan University, Kirtipur, Kathmandu, NepalCentral Department of Microbiology, Tribhuvan University, Kirtipur, Kathmandu, NepalDepartment of Pathology, Kirtipur Hospital, Kirtipur, Kathmandu, NepalCentral Department of Microbiology, Tribhuvan University, Kirtipur, Kathmandu, Nepal Background and Objectives: Staphylococcus aureus has increasingly been associated with community and healthcare-associated infections worldwide and contributes to treatment failures due to the emergence of multidrug-resistant (MDR) and methicillin-resistant Staphylococcus aureus (MRSA) strains. We aimed to determine the prevalence and antibiotic susceptibility pattern of MRSA isolated from patients attending a burn center and to detect mecA and PVL genes among MRSA isolates. Materials and Methods: A cross-sectional hospital based study was conducted on 1950 clinical samples collected from hospital inpatients and outpatients of Kirtipur Hospital, which is a burn specialist hospital in Kathmandu, Nepal. Each sample underwent conventional cultural methods for bacterial isolates identification. Results: Out of 1950 samples, 452 (23.2%) samples showed bacterial growth, of which 109 isolates (24.1%) were identified as Gram positive and 343 (75.9%) as Gram negative bacteria. Among the Gram positive bacteria, 53 (48.62%) were Staphylococcus aureus. Of the total S. aureus isolates, 40 (75.5%) were MRSA and 48 (90.6%) were MDR. Of the 40 MRSA isolates, 29 (72.5%) carried the mecA gene and 3 (7.5%) harbored PVL gene. Conclusion: The high prevalence of MRSA in a burn unit underscores the need for more rigorous infection control practices that follow standard protocols to reduce MRSA transmission in both individuals and the hospital environment. https://ijm.tums.ac.ir/index.php/ijm/article/view/5035Methicillin-resistant Staphylococcus aureus;MDR;mecA gene;PVL gene;Wound infection |
| spellingShingle | Sirjana Adhikari Supriya Sharma Sanjib Adhikari Sanjit Shrestha Dwij Raj Bhatta mecA and PVL genes in methicillin-resistant Staphylococcus aureus from clinical specimens: a cross-sectional hospital based study from Nepal Iranian Journal of Microbiology Methicillin-resistant Staphylococcus aureus; MDR; mecA gene; PVL gene; Wound infection |
| title | mecA and PVL genes in methicillin-resistant Staphylococcus aureus from clinical specimens: a cross-sectional hospital based study from Nepal |
| title_full | mecA and PVL genes in methicillin-resistant Staphylococcus aureus from clinical specimens: a cross-sectional hospital based study from Nepal |
| title_fullStr | mecA and PVL genes in methicillin-resistant Staphylococcus aureus from clinical specimens: a cross-sectional hospital based study from Nepal |
| title_full_unstemmed | mecA and PVL genes in methicillin-resistant Staphylococcus aureus from clinical specimens: a cross-sectional hospital based study from Nepal |
| title_short | mecA and PVL genes in methicillin-resistant Staphylococcus aureus from clinical specimens: a cross-sectional hospital based study from Nepal |
| title_sort | meca and pvl genes in methicillin resistant staphylococcus aureus from clinical specimens a cross sectional hospital based study from nepal |
| topic | Methicillin-resistant Staphylococcus aureus; MDR; mecA gene; PVL gene; Wound infection |
| url | https://ijm.tums.ac.ir/index.php/ijm/article/view/5035 |
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